Quantitative Determination of Nitrofurazone Metabolites in Animal-Derived Foods Based on a Background Fluorescence Quenching Immunochromatographic Assay

Abstract

Due to their facile synthesis and friendly functionalization, gold nanoparticles (AuNPs) have been applied in all kinds of biosensors. More importantly, these biosensors, with the combination of AuNPs and immunoassay, are expected to be used for the detection of different compounds with low concentrations in complex samples. In this study, a AuNPs-labeled antibody immunoprobe was prepared and combined with a fluorescence-quenching principle and a background fluorescence-quenching immunochromatographic assay (bFQICA), achieving rapid on-site detection. By using a portable fluorescence immunoquantitative analyzer and a QR code with a built-in standard curve, the rapid quantitative determination for nitrofurazone metabolite of semicarbazide (SEM) in animal-derived foods was realized. The limits of detection (LODs) for bFQICA in egg, chicken, fish, and shrimp were 0.09, 0.10, 0.12, and 0.15 μg kg−1 for SEM, respectively, with the linear range of 0.08–0.41 μg L−1, the recoveries ranging from 73.5% to 109.2%, and the coefficient of variation <15%, only taking 13 min for the SEM detection. The analysis of animal-derived foods by bFQICA complied with that of liquid chromatography-tandem mass spectrometry (LC-MS/MS).

A portable smartphone platform utilizing dual-sensing signals for visual determination of semicarbazide in food samples

“…The background fluorescence-quenching immunochromatographic assay (bFQICA) showed potential application for the quantitative determination of SEM in animal-derived foods. 51 Since the detection mode of this assay was a competitive reaction, environmental interference easily disturbed detection results. Zhang et al reported a freeze-synthesized bio-barcode immunoprotein-based multiplex fluorescence immunosensor to simultaneously determine four nitrofuran metabolites with a tedious preparation process.…”

Preparation of Anti-Aristolochic Acid I Monoclonal Antibody and Development of Chemiluminescent Immunoassay and Carbon Dot-Based Fluoroimmunoassay for Sensitive Detection of Aristolochic Acid I

“…Therefore, immunoassay was proposed in this study because of the advantages of rapidness, high-throughput, sensitivity, low-cost, simple pretreatment requirement. It is easy-to-use and has been widely applied in fields of food analysis [18][19][20][21][22][23][24].…”

Application of Antibody and Immunoassay for Food Safety

“…However, AuNPs-LFIA is known to have limited sensitivity because of AuNPs’ narrow particle size range and poor colloid stability. Wu et al [ 6 ] described a background fluorescence-quenching immunochromatographic assay (bFQICA) in which AuNPs were used to quench the fluorescence of a background fluorescence baseboard instead of using the colorimetric method. Such a method was optimized, validated, and applied in the rapid on-site detection of nitrofurazone metabolite of semicarbazide (SEM) residues in animal-derived foods (egg, chicken, fish, and shrimp).…”