2008
DOI: 10.1021/jf8012037
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Determination of Nucleotides in Infant Formula by Ion-Exchange Liquid Chromatography

Abstract: Nucleotide-supplemented infant formula has been shown to positively modify the composition of intestinal microflora, emulating the attribute of human milk. Quantification of nucleotides in infant formula is of interest because of its applicability in quality and safety assessments. There is no standard method for the analysis of nucleotides in infant formula. In the present study, ion-exchange liquid chromatography (IELC)- and centrifugal ultrafiltration (CUF)-based protocols were developed for routine determi… Show more

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Cited by 25 publications
(34 citation statements)
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“…A linear trend was obtained up to 60 s (about 450 nL of volume injected), while broaden peaks were obtained with 80 s. The best injection conditions showed LODs of 0.25 μg/mL for all the studied compounds. The values found were higher than those published in some works performed with HPLC and CE‐MS but they were in accord with the sensitivity requirements for nucleotides analysis in IF samples.…”
Section: Resultssupporting
confidence: 78%
“…A linear trend was obtained up to 60 s (about 450 nL of volume injected), while broaden peaks were obtained with 80 s. The best injection conditions showed LODs of 0.25 μg/mL for all the studied compounds. The values found were higher than those published in some works performed with HPLC and CE‐MS but they were in accord with the sensitivity requirements for nucleotides analysis in IF samples.…”
Section: Resultssupporting
confidence: 78%
“…The treatment selected was subsequently optimized. First, the amount of powdered milk sample was studied (5.0, 1.0, and 0.5 g).…”
Section: Resultsmentioning
confidence: 99%
“…They can enhance immunity and decrease the risk of diarrhea and sepsis . The addition of 5′‐ribonucleotides in infant formula can improve the composition of intestinal microflora, emulating the attibute of human milk …”
Section: Introductionmentioning
confidence: 99%
“…The mixture of four 5′‐ribonucleotides is obtained after enzymatic hydrolysis. An ion exchange technique is a potential method to separate and purify 5′‐ribonucleotides . When anion exchangers are used to separate 5′‐ribonucleotides, gradient elution is usually needed with some weak acids including formic acid and acetic acid used as eluant .…”
Section: Introductionmentioning
confidence: 99%