Determination of phenolic acids and flavonoids in Rhinacanthus nasutus (L.) kurz by high-performance-liquid-chromatography with photodiode-array detection and tandem mass spectrometry

Huang, Rong-Qing; Lu, Yasong; Inbaraj, Baskaran Stephen; Chen, B.H.

doi:10.1016/j.jff.2014.12.002

Cited by 39 publications

(25 citation statements)

References 41 publications

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“…Phenolic acids are widely present in plant materials, as secondary metabolites in the free form or linked to glycosides of soluble and insoluble esters, presenting biological activities, such as anticancer, antibacterial and antioxidant activity (HUANG et al, 2015). Phenolic compounds provide cell wall support materials (lignin) and under normal maturation conditions, their levels are higher during fruit growth and decrease with maturation (SELVARAJ and KUMAR, 1989).…”

Section: Resultsmentioning

confidence: 99%

MINERALS AND PHENOLIC COMPOUNDS OF CAGAITA FRUITS AT DIFFERENT MATURATION STAGES (Eugenia dysenterica)

et al. 2017

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-Cerrado is the largest biome in the state of Minas Gerais-Brazil, represented by a biodiversity of fruit species, especially cagaita (Eugenia dysenterica). Cagaita fruits are considered an important option for fruit growing, because they are source of phenolic compounds with antioxidant properties and mineral content that can contribute with beneficial health effects. In this study, phenolic compounds were characterized by high-performance liquid chromatography and the mineral content of ripe and unripe cagaita fruits was evaluated. Regarding minerals, ripe and unripe fruits stand out due to their high potassium content. Unripe fruits are rich in boron while ripe fruits are source of this mineral. ripe and unripe cagaita fruits have the following phenolic compounds: gallic, caffeic, vanillic, p-coumaric, siringeic, ferulic and salicylic acids, epicatechin, quercetin and rutin, the highest contents of these compounds are caffeic, p-coumaric, siringeic and ferrulic, epicatechin and rutin identified in unripe fruits, while ripe fruits have high contents of gallic, vanillic and quercetin acids. Thus, cagaita fruits have great potential for food, therapeutic and medicinal applications bringing health benefits. Index terms: Cerrado fruits, Myrtaceae, HPLC-Uv. MINERAIS E COMPOSTOS FENÓLICOS EM DIFERENTES ESTÁDIOS DE MATURAÇÃO DE FRUTOS DE CAGAITEIRA (Eugenia dysenterica)RESUMO -O Cerrado é o maior bioma no Estado de Minas Gerais-Brasil, representado uma biodiversidade de espécies frutíferas, destacando a cagaiteira (Eugenia dysenterica). Frutos de cagaiteira são considerados uma opção importante para fruticultura, por apresentarem fonte de compostos fenólicos com propriedades antioxidantes e teores de minerais que podem contribuir com efeitos benéficos à saúde. Neste estudo, caracterizaram-se os compostos fenólicos por cromatografia líquida de alta eficiência e avaliaram-se os teores de minerais presentes em frutos de cagaiteira em estádio de maturação maduros e verdes. Em relação aos minerais, frutos maduros e verdes destacam-se por serem ricos em potássio. Os frutos verdes são ricos em boro, enquanto os frutos maduros são fonte desse mineral. Nos frutos de cagaiteira maduros e verdes, foram identificados os compostos fenólicos: os ácidos gálico, cafeico, vanílico, p-cumárico, siríngico, ferúlico e salicílico, epicatequina, quercetina e rutina, sendo os maiores teores dos compostos os ácidos cafeico, p-cumárico, siríngico e ferúlico, epicatequina e rutina identificados nos frutos verdes, enquanto para frutos maduros o destaque foi para os ácidos gálico, vanílico esalicílico e quercetina. Assim, frutos de cagaita podem apresentar grande potencial para aplicações alimentares, terapêuticas e medicinais, trazendo benefícios à saúde. Termos de indexação:Frutos do cerrado, Myrtaceae, CLAE-Uv.

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Section: Resultsmentioning

confidence: 99%

MINERALS AND PHENOLIC COMPOUNDS OF CAGAITA FRUITS AT DIFFERENT MATURATION STAGES (Eugenia dysenterica)

et al. 2017

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“…Rhinacanthus nasutus (L.) Kurz ( R. nasutus ) is a well-known Chinese medicinal herb widely grown in Asian countries such as Taiwan and China, and often sold as “healthy beverage” on the market 13 . Several vital biological activities including anti-cancer 14 , anti-bacteria 15 and anti-inflammation 16 for consumption of R. nasutus have been well documented, which can be attributed to presence of various functional compounds like carotenoids, flavonoids, phenolic acids, chlorophylls, and napthoquinones 13 17 18 19 . Of various functional compounds, the carotenoid composition in R. nasutus has been thoroughly studied by Kao et al 18 and reported a total amount of 2195 μ g/g and 1576 μ g/g in freeze-dried and hot-air-dried samples, respectively.…”

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confidence: 99%

Utilization of Microemulsions from Rhinacanthus nasutus (L.) Kurz to Improve Carotenoid Bioavailability

Inbaraj

Chen

2016

Sci Rep

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Carotenoids have been known to reduce the risk of several diseases including cancer and cardiovascular. However, carotenoids are unstable and susceptible to degradation. Rhinacanthus nasutus (L.) Kurz (R. nasutus), a Chinese medicinal herb rich in carotenoids, was reported to possess vital biological activities such as anti-cancer. This study intends to isolate carotenoids from R. nasutus by column chromatography, identify and quantify by HPLC-MS, and prepare carotenoid microemulsions for determination of absolute bioavailability in rats. Initially, carotenoid fraction was isolated using 250 mL ethyl acetate poured into an open-column packed with magnesium oxide-diatomaceous earth (1:3, w/w). Fourteen carotenoids including internal standard β-apo-8′-carotenal were resolved within 62 min by a YMC C30 column and gradient mobile phase of methanol-acetonitrile-water (82:14:4, v/v/v) and methylene chloride. Highly stable carotenoid microemulsions were prepared using a mixture of CapryolTM90, Transcutol®HP, Tween 80 and deionized water, with the mean particle being 10.4 nm for oral administration and 10.7 nm for intravenous injection. Pharmacokinetic study revealed that the absolute bioavailability of carotenoids in microemulsions and dispersion was 0.45% and 0.11%, respectively, while a much higher value of 6.25% and 1.57% were shown for lutein, demonstrating 4-fold enhancement in bioavailability upon incorporation of R. nasutus carotenoids into a microemulsion system.

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“…Rhinacanthus nasutus is a medicinal herb rich in carotenoids that has anticancer activity [121]. Ho et al [122] isolated the carotenoids from R. nasutus to prepare microemulsions with enhanced oral bioavailability in rats.…”

Section: Enhancement Of Oral Bioavailability Of Vitamins Using Lipmentioning

confidence: 99%

Use of Lipid Nanocarriers to Improve Oral Delivery of Vitamins

et al. 2019

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The chemical environment and enzymes in the gastrointestinal (GI) membrane limit the oral absorption of some vitamins. The GI epithelium also contributes to the poor permeability of numerous antioxidant agents. Thus, lipophilic vitamins do not readily dissolve in the GI tract, and therefore they have low bioavailability. Nanomedicine has the potential to improve the delivery efficiency of oral vitamins. In particular, the use of lipid nanocarriers for certain vitamins that are administered orally can provide improved solubility, chemical stability, epithelium permeability and bioavailability, half-life, nidus targeting, and fewer adverse effects. These lipid nanocarriers include self-emulsifying drug delivery systems (SEDDSs), nanoemulsions, microemulsions, solid lipid nanoparticles (SLNs), and nanostructured lipid carriers (NLCs). The use of nontoxic excipients and sophisticated material engineering of lipid nanosystems allows for control of the physicochemical properties of the nanoparticles and improved GI permeation via mucosal or lymphatic transport. In this review, we highlight recent progress in the development of lipid nanocarriers for vitamin delivery. In addition, the same lipid nanocarriers used for vitamins may also be effective as carriers of vitamin derivatives, and therefore enhance their oral bioavailability. One example is the incorporation of d-α-tocopheryl polyethylene glycol succinate (TPGS) as the emulsifier in lipid nanocarriers to increase the solubility and inhibit P-glycoprotein (P-gp) efflux. We also survey the concepts and discuss the mechanisms of nanomedical techniques that are used to develop vitamin-loaded nanocarriers.

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Determination of phenolic acids and flavonoids in Rhinacanthus nasutus (L.) kurz by high-performance-liquid-chromatography with photodiode-array detection and tandem mass spectrometry

Cited by 39 publications

References 41 publications

MINERALS AND PHENOLIC COMPOUNDS OF CAGAITA FRUITS AT DIFFERENT MATURATION STAGES (Eugenia dysenterica)

MINERALS AND PHENOLIC COMPOUNDS OF CAGAITA FRUITS AT DIFFERENT MATURATION STAGES (Eugenia dysenterica)

Utilization of Microemulsions from Rhinacanthus nasutus (L.) Kurz to Improve Carotenoid Bioavailability

Use of Lipid Nanocarriers to Improve Oral Delivery of Vitamins

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