Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography–ultraviolet detection analysis

Mateo, Rafael; Castells, G.; Green, Andy J.; Godoy, Carmen Gloria; Cristòfol, Carles

doi:10.1016/j.jchromb.2004.08.019

Cited by 25 publications

(31 citation statements)

References 24 publications

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“…Biliverdin analysis was based on the method described by Mateo et al (2004) with some modifications. Biliverdin was extracted from 7-to 19-mg eggshell pieces from the equatorial region of one egg (fourth) per clutch by adding 315 ll acetonitrile and 263 ll HCl 3N.…”

Section: Biliverdin Analysismentioning

confidence: 99%

Shell thickness and pore density in relation to shell colouration, female characteristics, and environmental factors in the Collared Flycatcher Ficedula albicollis

2010

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Avian eggshell structure may have important consequences for embryonic growth and development, but relatively little is known about the factors responsible for variation in eggshell characteristics of wild birds. In this paper, we explored potential causes of variation in eggshell colour and structure (shell thickness and porosity) in the Collared Flycatcher (Ficedula albicollis). We analysed if eggshell colour is affected by shell structure or pigment level, and whether female traits, laying date, local breeding density, and clutch size affect shell thickness and pore density. We found that eggshell blue-green and UV colours were unrelated to shell thickness, pore density and egg size. Eggs with higher concentration of biliverdin showed lower UV reflection and higher reflection in the blue-green part of the spectrum. We found that females in better nutritional condition, indicated by their higher mass controlled for tarsus length, laid eggs with thicker shells. It is possible that females in better condition have more time available for searching calcium-rich food, and thus could produce eggs with stronger shells. However, female physical characteristics had no significant relationships with shell porosity. In contrast to our expectation, shell thickness and pore density were unrelated to local breeding density and laying date, though very late and repeat clutches were not sampled in our study. However, we found that eggs in larger clutches had lower pore density than eggs in smaller clutches, which may be expected if the rate of water loss and nest humidity are to remain constant in clutches of different egg numbers.Zusammenfassung Die Struktur der Eischale von Vögeln kann wichtige Folgen für das Wachstum und die Entwicklung der Embryonen haben, doch über die Faktoren, die für Variation in den Eigenschaften der Eischale bei freilebenden Vögeln verantwortlich sind, ist relativ wenig bekannt. In diesem Artikel haben wir mögliche Gründe für die Variation in Eischalenfarbe und -struktur (Schalendicke und Porosität) beim Halsbandschnäpper (Ficedula albicollis) untersucht. Wir haben analysiert, ob die Eischalenfarbe von der Schalenstruktur oder dem Pigmentgehalt beeinflusst wird und ob Weibchenmerkmale, Legedatum, lokale Brutpaardichte und Gelegegröße die Schalendicke und Porendichte beeinflussen. Wir fanden, dass blaugrüne und ultraviolette Farben der Eischale nicht mit der Schalendicke, Porendichte und Eigröße zusammenhingen. Eier mit einer höheren Biliverdinkonzentration zeigten eine geringere UV-Reflexion und stärkere Reflexion im blaugrünen Bereich des Spektrums. Wir fanden, dass Weibchen in besserem Ernährungszustand, angezeigt durch ihre höhere Körpermasse korrigiert für Tarsuslänge, Eier

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Section: Biliverdin Analysismentioning

confidence: 99%

Shell thickness and pore density in relation to shell colouration, female characteristics, and environmental factors in the Collared Flycatcher Ficedula albicollis

2010

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“…We determined eggshell porphyrins and biliverdin levels following the method described in Mateo et al (2004) with some modifications (See SI for more details).…”

Section: Measurement Of Thickness and Pigment Concentrations In Eggshmentioning

confidence: 99%

Altered immune response in mallard ducklings exposed to lead through maternal transfer in the wild

Vallverdú-Coll

López-Antia

Martínez-Haro

et al. 2015

Environmental Pollution

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“…Porphyrins and biliverdin were determined in excreta and bile of geese by high-pressure liquid chromatography with ultraviolet detection [14]. As porphyrins and biliverdin have a common biliary origin in bird excreta, porphyrin concentrations in excreta were expressed relative to biliverdin in order to compensate for variation in the amount of feces excreted due to variation in the diet.…”

Section: Chemical Analysesmentioning

confidence: 99%

“…Two of the elements in higher concentrations in the contaminated Aznalcóllar sludge were Pb and Zn; thus, the risk of poisoning of waterfowl inhabiting the Guadalquivir Marshes may resemble the scenario of the Coeur d'Alene River basin in Idaho, USA, where analyses of fecal excreta were used to relate soil ingestion to the exposure to heavy metals in birds [13]. Moreover, this noninvasive sampling provides evidence of toxic effects of heavy metals and As in birds from the analysis of porphyrins and biliverdin in fecal excreta [14].…”

Section: Introductionmentioning

confidence: 99%

Altered porphyrin excretion and histopathology of greylag geese (Anser anser) exposed to soil contaminated with lead and arsenic in the guadalquivir marshes, southwestern Spain

Mateo

Taggart

Green

et al. 2006

Enviro Toxic and Chemistry

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Greylag geese (Anser anser) in the Guadalquivir Marshes (southwestern Spain) can be exposed to sources of inorganic pollution such as heavy metals and arsenic from mining activities or Pb shot used for hunting. We have sampled 270 fecal excreta in different areas of the marshes in 2001 to 2002 to evaluate the exposure to Pb, Zn, Cu, Mn, and As and to determine its relationship with soil ingestion and with the excretion of porphyrins and biliverdin as biomarkers. These effects and the histopathology of liver, kidney, and pancreas were also studied in 50 geese shot in 2002 to 2004. None of the geese had ingested Pb shot in the gizzard. This contrasts with earlier samplings before the ban of Pb shot for waterfowl hunting in 2001 and the removal of Pb shot in points of the Doñana National Park (Spain) in 1999 to 2000. The highest exposure through direct soil ingestion to Pb and other studied elements was observed in samples from Entremuros, the area of the Doñana Natural Park affected by the Aznalcóllar mine spill in 1998. Birds from Entremuros also more frequently showed mononuclear infiltrates in liver and kidney than birds from the unaffected areas, although other more specific lesions of Pb or Zn poisoning were not observed. The excretion of coproporphyrins, especially of the isomer I, was positively related to the fecal As concentration, and the ratio of coproporphyrin III/I was positively related to fecal Pb concentration. Biliary protoporphyrin IX concentration was also slightly related to hepatic Pb concentration. This study reflects biological effects on terrestrial animals by the mining pollution in Doñana that can be monitored with the simple noninvasive sampling of feces.

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Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography–ultraviolet detection analysis

Cited by 25 publications

References 24 publications

Shell thickness and pore density in relation to shell colouration, female characteristics, and environmental factors in the Collared Flycatcher Ficedula albicollis

Shell thickness and pore density in relation to shell colouration, female characteristics, and environmental factors in the Collared Flycatcher Ficedula albicollis

Altered immune response in mallard ducklings exposed to lead through maternal transfer in the wild

Altered porphyrin excretion and histopathology of greylag geese (Anser anser) exposed to soil contaminated with lead and arsenic in the guadalquivir marshes, southwestern Spain

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