Determination of propofol in low-volume samples by high-performance liquid chromatography with fluorescence detection

Quantitative measurement of propofol and in main glucuroconjugate metabolites in human plasma using solid phase extraction–liquid chromatography–tandem mass spectrometry

“…The quantification of propofol in human whole blood or plasma commonly employs liquid chromatography (coupled with UV, fluorescence, or electrochemical detection) although several assays using gas chromatography have also been described [6][7][8][9]. Recently, liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) techniques were described for the determination of propofol using either APCI without derivatization or ESI with derivatization step [10,11].…”

Gas chromatography–mass spectrometric assay for propofol in cerebrospinal fluid of traumatic brain patients

“…The method of high pressure liquid chromatography with fluorescence detection, which has the advantage of low costs and wide availability, is not sensitive enough to cover the concentration range of interest in CSF at propofol infusion rates of ≤5 mg kg −1 h −1 . The LOQ for a precipitation method was as high as 30 g/L for whole blood [5,8]. Only after evaporation to dryness beyond extraction, a limit of detection of 1.1 g/L in CSF has been achieved [9][10][11].…”

“…Propofol concentrations in whole blood were measured using high-performance liquid chromatography with fluorescence detection [5]. The limit of quantification was 0.035 mg/L.…”

Pharmacokinetics of Propofol and Extrahepatic UGT1A6 Gene Expression in Anhepatic Rats

“…The concentrations of propofol in microvolume blood samples were measured with the established method previously reported by high-performance liquid chromatography (HPLC) [16,17] . In order to establish this method in our laboratory, the essential studies have been conducted with series of standard concentrations of propofol at 50, 25, 12.5, 5, 2.5, 1.25, 0.5 and 0.25 g/ml in methanol.…”