Determination of residual pharmaceuticals in edible animal tissues by continuous solid-phase extraction and gas chromatography–mass spectrometry

Azzouz, Abdelmonaim; Souhail, Badredine; Ballesteros, Evaristo

doi:10.1016/j.talanta.2011.02.016

Cited by 60 publications

(24 citation statements)

References 28 publications

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“…However, the number of papers published reporting the occurrence and levels of PhACs in marine organisms is considerably lower. They have been performed mainly in mollusks (Wille et al, 2011;McEneff et al, 2014;Klosterhaus et al, 2013;Martinez Bueno et al, 2013Li et al, 2012;Dodder et al, 2014) and freshwater fish (Gelsleichter and Szabo, 2013;Maruya et al, 2012;Azzouz et al, 2011). The levels found are usually in the low ng/g (i.e.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of pharmaceuticals and endocrine disrupting compounds in macroalgaes, bivalves, and fish from coastal areas in Europe

Álvarez-Muñoz

Rodríguez-Mozaz

Maulvault

et al. 2015

Environmental Research

224

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Section: Introductionmentioning

confidence: 99%

Occurrence of pharmaceuticals and endocrine disrupting compounds in macroalgaes, bivalves, and fish from coastal areas in Europe

Álvarez-Muñoz

Rodríguez-Mozaz

Maulvault

et al. 2015

Environmental Research

224

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“…Common approaches for the extraction of pharmaceutically active compounds (PhACs) from fish tissues rely on solid-liquid extraction, enzymatic microwave-assisted extraction, ultra-sound extraction (USE), QuEChERS, and pressurized liquid extraction [1 , [3] , [4] , [5] , [6] , [7] , [8] , [9] , [10] . Prior to the analysis of the extracts by LC-MS, it is critical to remove co-extracted lipids as much as possible in order to reduce matrix effects during analyte ionization in the interface.…”

Section: *Methods Detailsmentioning

confidence: 99%

Analysis of pharmaceuticals in fish using ultrasound extraction and dispersive spe clean-up on que Z-Sep/C18 followed by LC-QToF-MS detection

et al. 2020

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The presence of pharmaceutically active compounds (PhACs) in aquatic biota has been received much less attention than their presence in surface or waste water, and it was not until the mid-2000s, this gap started to be addressed. Here, we present SQUEEZe ( S olid-li Q uid U ltrasound E xtraction with Qu E Z -S e p/C18 as dispersive clean-up): a fast method for analysis of the trace 47 PhACs in fish muscle. Compared to our previously reported method [1] , it offers alternatives with improvements in recoveries, number of analytes, sample volume and solvent used. The key aspects of this method are: The ultrasound extraction was performed with acetonitrile/isopropanol 0.1% V/V formic acid. A clean-up step using QuE Z-Sep/C18 sorbents was employed to reduce lipid content of the extracts and further matrix effects in the detection of the analytes. A HPLC separation with a Kinetex EVO C18 packed column in 11 min was optimized. MS and MS/MS data were collected using SWATH acquisition on the SCIEX X500R QTOF in (+)-ESI mode. The method validated at 3 different concentrations levels: 5, 25 and 50 ng/g fish. It presented good intraday/interday reproducibility and absolute recoveries ≥ 60% for majority of analytes in composite homogenate muscle matrix of Squalius cephalus . 10 out 47 compounds were detected in fish samples.

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“…Several analytical methods have been reported for the determination of either sulfaquinoxaline-Na or pyrimethamine in their pharmaceutical formulations and/or in biological fluids. The literature comprises spectrophotometric [4][5][6][7][8] , electrochemical [9][10][11][12] , biochemical [13][14][15] and chromatographic techniques namely, densitometry [16][17][18][19] , GC [20][21][22] and HPLC [23][24][25][26][27][28][29] . Also, capillary electrophoresis [30][31][32] was revealed for sulfaquinoxaline-Na determination.…”

Section: Introductionmentioning

confidence: 99%

Densitometric and Ratio Spectra Methods for Simultaneous Determination of Sulfaquinoxaline Sodium and Pyrimethamine in Binary Mixture

Razeq

Demerdash

Fouad

et al. 2019

Bulletin of Faculty of Pharmacy, Cairo University

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Four methods were developed for simultaneous determination of sulfaquinoxaline-Na and pyrimethamine in their combined pharmaceutical formulations. The first one was a densitometric method where chloroform-methanol (9: 1, v/v) was the developing system and the plates were scanned at 254 nm. The obtained spots appeared at Rf 0.64 and 0.35 and determined in the range of 0.5-10.0 µg/spot and 0.1-10.0 µg/spot for sulfaquinoxaline-Na and pyrimethamine, respectively. Furthermore, three spectrophotometric methods manipulating ratio spectra namely, ratio difference method, extended ratio subtraction method coupled with ratio subtraction method and mean centering method were established for the determination of the two studied drugs in the presence of propylene glycol as a solvent. Linear correlation was found over the concentration range of 2-25 µg mL-1 and 3-15 µg mL-1 for the two drugs, respectively. The proposed methods were successfully applied for analyzing the cited drugs in their veterinary pharmaceutical formulations. The obtained results were statistically analyzed and found to be in accordance with those given by a reported method. The validity of the methods was evaluated according to ICH guidelines.

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Determination of residual pharmaceuticals in edible animal tissues by continuous solid-phase extraction and gas chromatography–mass spectrometry

Cited by 60 publications

References 28 publications

Occurrence of pharmaceuticals and endocrine disrupting compounds in macroalgaes, bivalves, and fish from coastal areas in Europe

Occurrence of pharmaceuticals and endocrine disrupting compounds in macroalgaes, bivalves, and fish from coastal areas in Europe

Analysis of pharmaceuticals in fish using ultrasound extraction and dispersive spe clean-up on que Z-Sep/C18 followed by LC-QToF-MS detection

Densitometric and Ratio Spectra Methods for Simultaneous Determination of Sulfaquinoxaline Sodium and Pyrimethamine in Binary Mixture

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