Determination of Residual Species Serum Albumin in Adulterated Ground Beef

Abstract

Species-specific antisera were used in agar-gel precipitin reactions to determine the presence of serum proteins from pig, horse and rabbit flesh in saline extracts of fresh ground beef that contained flesh from these species. Precipitin lines of identity were observed between species I serum and extracts obtained from ground beef which contained 1, 3 and 5% species flesh. No reactions were observed with control bovine serum. The results indicated that serum albumin was the major protein detected for each species. This method could be used for the qualitative detection of species adulterants in fresh ground beef.

Development of monoclonal antibody for rock shrimp identification using enzyme-linked immunosorbent assay

“…Immunological assays, as an alternative, can be used to reduce the test time and cost, as well as to increase the sensitivity in detecting food components (Hayden, 1977(Hayden, , 1978(Hayden, ,1981Hitchcock et al, 1981;Karpas et al, 1970;Skerritt, 1985). Lundstrom (1984Lundstrom ( , 1985, using hybridoma technology, developed the enzyme-linked immunosorbent assay (ELISA) to identify fish species and to differentiate fish stock.…”

“…Immunological assays, as an alternative, can be used to reduce the test time and cost, as well as to increase the sensitivity in detecting food components (Hayden, 1977(Hayden, , 1978(Hayden, ,1981; Hitchcock et al, 1981;Karpas et al, 1970;Skerritt, 1985). Lundstrom (1984Lundstrom ( , 1985, using hybridoma technology, developed the enzyme-linked immunosorbent assay (ELISA) to identify fish species and to ELISA has been widely used to differentiate fish species and unprocessed beef, pig, horse, sheep, kangaroo, camel, goat, and buffalo meat (Kang'ethe et al, 1982; Lundstrom, 1984Lundstrom, , 1985; Patterson et al, 1984;Skerritt, 1985;Whittaker et al, 1983).…”