Determination of roquefortine C in blue cheese using on-line column-switching liquid chromatography

Noroozian, Ebrahim; Lagerwerf, Fija M.; Lingeman, H.; Brinkman, U.A.T.; Kerkhoff, Mia A.T.

doi:10.1016/s0731-7085(98)00087-9

Cited by 10 publications

(6 citation statements)

References 8 publications

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“…Finally, mycotoxins can be degraded upon exposure to light, yielding products of unknown toxicity. This has been shown for roquefortine C in cheese [24] and for ochratoxin A in wines [118]. …”

Section: Pitfalls Of Mycotoxin Analysismentioning

confidence: 62%

“…Detection wavelength was 254 nm [23]. Roquefortine C in cheese was analyzed by on-line extraction and separation on C 18 column, λ 330 nm [24]. Alternaria mycotoxins can be separated on reverse-phase column with UV detection at 240–260 nm [230].…”

Section: Methodsmentioning

confidence: 99%

“…As the sample preparation is frequently the crucial step in the determination of mycotoxins, some on-line extraction procedures, requiring less manipulation of the sample, were shown to be better compared to the off-line approach [24, 58, 118]. Most SPE procedures include the following steps: column conditioning, sample loading, column washing and analyte elution.…”

Section: Sample Preparation and Pre-concentrationmentioning

confidence: 99%

“…Balkan endemic nephropathy [19, 21], other environmental factors such as drinking water contamination may also play a role in their occurrence [22]. At present, there is no firm evidence that the normal daily exposure to some mycotoxins listed in Table 1 (RQ, MPA) would lead to health disorders [23, 24] or these occur only at higher intake levels (e.g. for PAT) [25], but nevertheless the maximum tolerable daily intake and residual levels in foods have been established [1, 25].…”

Section: Introductionmentioning

confidence: 99%

“…Other mycotoxins haven’t been shown to exhibit carry-over from feed to animal products [1, 3]. Cheese deliberately inoculated with presumably non-toxicogenic molds may in addition contain MPA, RQ [23, 24] and to a certain extent also CIT and CPA are expected [19, 27]. Fish may contain RALs [28].…”

Section: Introductionmentioning

confidence: 99%

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An Overview of Conventional and Emerging Analytical Methods for the Determination of Mycotoxins

Cigić

Prosen

2009

IJMS

179

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Abstract:Mycotoxins are a group of compounds produced by various fungi and excreted into the matrices on which they grow, often food intended for human consumption or animal feed. The high toxicity and carcinogenicity of these compounds and their ability to cause various pathological conditions has led to widespread screening of foods and feeds potentially polluted with them. Maximum permissible levels in different matrices have also been established for some toxins. As these are quite low, analytical methods for determination of mycotoxins have to be both sensitive and specific. In addition, an appropriate sample preparation and pre-concentration method is needed to isolate analytes from rather complicated samples. In this article, an overview of methods for analysis and sample preparation published in the last ten years is given for the most often encountered mycotoxins in different samples, mainly in food. Special emphasis is on liquid chromatography with fluorescence and mass spectrometric detection, while in the field of sample preparation various solid-phase extraction approaches are discussed. However, an overview of other analytical and sample preparation methods less often used is also given. Finally, different matrices where mycotoxins have to be determined are discussed with the emphasis on their specific characteristics important for the analysis (human food and beverages, animal feed, biological samples, environmental samples). Various issues important for accurate qualitative and quantitative analyses are critically discussed: sampling and choice of representative sample, sample preparation and possible bias associated with it, specificity of the analytical method and critical evaluation of results.

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Section: Pitfalls Of Mycotoxin Analysismentioning

confidence: 62%

Section: Methodsmentioning

confidence: 99%

Section: Sample Preparation and Pre-concentrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An Overview of Conventional and Emerging Analytical Methods for the Determination of Mycotoxins

Cigić

Prosen

2009

IJMS

179

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Isolation and metabolite production by Penicillium roqueforti, P. paneum and P. crustosum isolated in Canada

2005

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Penicillium roqueforti, P. crustosum and P. paneum grow on ensiled grain and recycled feed unless properly treated. The former two species occur also on cut lumber in Canada. These are known to produce a number of secondary metabolites including roquefortine. In cooler dairy production areas, including Scandinavia and North America, cattle toxicosis has been associated with silage contaminated by these fungi. We collected strains associated with cow or cattle toxicoses. The principal metabolites were determined making use of a new extraction method and analysis combining HPLC, LC/MS/MS, and LC/NMR. Penicillium roqueforti and P. crustosum required amino acid nitrogen for metabolite formation and their toxins were formed under conditions of low oxygen (20-30% saturation). Production of roquefortine C occurred on depletion of the available nitrogen and penitrem A on depletion of carbon source. Yield was reduced by excess carbon. Medium osmotic tension (aw) affected metabolite production by the two species differently. Penicillium paneum was associated with ill-thrift of dairy cows and P. roqueforti was associated with more serious symptoms. Our data suggest a physiological basis for the common occurrence of roquefortine C in silage without serious consequences and the alternative, the presence of roquefortine C and toxicoses. The strain isolated from lumber was the best producer of the toxins studied. This is the first report of the toxigenic potential of P. roqueforti and P. paneum from Canada.

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Application of Ambient Ionization Mass Spectrometry to Detect the Mycotoxin Roquefortine C in Blue Cheese

Maragos

2021

Food Anal. Methods

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Determination of roquefortine C in blue cheese using on-line column-switching liquid chromatography

Cited by 10 publications

References 8 publications

An Overview of Conventional and Emerging Analytical Methods for the Determination of Mycotoxins

An Overview of Conventional and Emerging Analytical Methods for the Determination of Mycotoxins

Isolation and metabolite production by Penicillium roqueforti, P. paneum and P. crustosum isolated in Canada

Application of Ambient Ionization Mass Spectrometry to Detect the Mycotoxin Roquefortine C in Blue Cheese

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