Determination of S Fimbriae Among Escherichia coli Strains from Extraintestinal Infections by Colony Hybridization and Dot Enzyme Immunoassay

Schönian, Gabriele; Sokolowska-Köhler, W; Bollmann, R.; Schubert, A; Gräser, Yvonne; Presber, W

doi:10.1016/s0934-8840(11)80014-9

Cited by 11 publications

(16 citation statements)

References 20 publications

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“…Reprinted from Johnson et al (2002b), with permission. Yes (Arthur et al, 1989b;O'Hanley et al, 1985) Yes (Roberts et al, 1994); no (Mobley et al, 1993) sfa/foc S and F1C fimbriae Yes (Scho¨nian et al, 1992) Yes (Marre and Hacker, 1987) sfa S fimbriae (sialic acid-specific) Yes (Johnson et al, 2002c) Yes (Marre and Hacker, 1987) foc F1C fimbriae Yes (Pere et al, 1985) N o afa/dra Dr antigen-specific adhesin operons (AFA I-III, Dr, F1845)…”

Section: Associations Among Virulence Factorsmentioning

confidence: 99%

Molecular epidemiology of extraintestinal pathogenic (uropathogenic) Escherichia coli

Johnson

Russo

2005

International Journal of Medical Microbiology

226

209

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Section: Associations Among Virulence Factorsmentioning

confidence: 99%

Molecular epidemiology of extraintestinal pathogenic (uropathogenic) Escherichia coli

Johnson

Russo

2005

International Journal of Medical Microbiology

226

209

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“…Two Pst I fragments of the recombinant plasmid pANN 801‐13 were used as polynucleotide probes for the detection of S/F1C sequences, as described earlier [17]. An alkali lysis method was used for large‐scale preparation of plasmid DNA [18].…”

Section: Methodsmentioning

confidence: 99%

“…The membranes were incubated overnight at 37°C. The extraction of bacterial DNA, the fixation to the filters, and the hybridization with radioactive or non‐radioactive labelled polynucleotide DNA probe were carried out as described previously [17]. With the oligonucleotide probes filter hybridization was performed overnight at temperatures 5°C below the melting temperature of the oligonucleotides.…”

Section: Methodsmentioning

confidence: 99%

“…Epidemiological investigations of E. coli virulence factors have often been based on phenotypic assays which are affected by variation in expression. Previously, using a DNA hybridization technique we were able to show that the phenotypic assay for S fimbriae is not sensitive since many pathogenic E. coli strains do not form these fimbriae under laboratory conditions [17]. As the probe used in this previous study was not able to distinguish between S and F1C fimbrial sequences, three oligonucleotides based on the sequence of the S fimbrial adhesin gene [18]were synthesized which hybridize specifically to S fimbriae determinants and do not cross‐react with F1C and S‐related fimbrial sequences.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of S and F1C/S-related fimbrial determinants and their expression inEscherichia colistrains isolated from extraintestinal infections: Table 1

Sokolowska-Köhler¹,

Schönian²,

Bollmann³

et al. 1997

FEMS Immunol Med Microbiol

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The presence of S and F1C/S-related fimbrial determinants was determined in 462 E. coli strains obtained from different extraintestinal infections and in 162 control isolates of E. coli by using two different DNA probes: an oligonucleotide probe consisting of three oligonucleotides that bind specifically to the S adhesin gene and a polynucleotide probe which is not able to distinguish between S, F1C, and S-related sequences. The expression of S and F1C phenotypes was tested by dot enzyme immunoassay with the corresponding monoclonal antibodies. S fimbriae genotypes were observed more frequently in septic (25%) and urinary (12%) isolates of E. coli than in faecal and water isolates (1%) and often occurred together with O2, O6, O18 and O83 antigens. F1C/S-related fimbrial DNA was detected with a higher frequency in UTI isolates (26%) than in septic (16%) and faecal (10%) isolates and was most frequently associated with O4, O6, and O75 serotypes. Since the production of S and F1C fimbriae was comparatively rare in all clinical and control isolates of E. coli, DNA hybridization assays which allow the sensitive and specific detection of fimbrial determinants even in the absence of their expression are preferable to phenotypic assays.

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“…Adhesins c pap Pilus associated with pyelonephritis (P fimbriae) Yes ( Arthur et al, 1989 ;O'Hanley et al, 1985 ) Yes ( Roberts et al, 1994 ); no ( Mobley et al, 1993 ) sfa/foc S and F1C fimbriae Yes ( Sch ö nian et al, 1992 ) Yes ( Marre and Hacker, 1987 ) sfa S fimbriae (sialic acid-specific)…”

Section: Experimental (In Vivo) Bmentioning

confidence: 99%

Extraintestinal Pathogenic Escherichia coli

Russo¹,

Johnson

2009

Vaccines for Biodefense and Emerging and Neglected Diseases

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Determination of S Fimbriae Among Escherichia coli Strains from Extraintestinal Infections by Colony Hybridization and Dot Enzyme Immunoassay

Cited by 11 publications

References 20 publications

Molecular epidemiology of extraintestinal pathogenic (uropathogenic) Escherichia coli

Molecular epidemiology of extraintestinal pathogenic (uropathogenic) Escherichia coli

Occurrence of S and F1C/S-related fimbrial determinants and their expression inEscherichia colistrains isolated from extraintestinal infections: Table 1

Extraintestinal Pathogenic Escherichia coli

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