Determination of secondary amines as highly fluorescent formamidines by high-performance liquid chromatography

Khalaf, Hosni; Steinert, J.

doi:10.1016/s0003-2670(96)00295-4

Cited by 9 publications

(3 citation statements)

References 10 publications

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“…To overcome this problem, amines can be stabilized through derivatisation, followed by high-performance liquid chromatography analysis using ultraviolet (UV) or fluorescence (FL) detection. [10][11][12] Currently, different derivatisation agents are available, [13][14][15][16][17][18][19] 1-naphthylisothiocyanate (NIT) appears to be the most frequently used reagent for amine derivatisation. [20][21][22][23][24][25][26][27] However, this reagent does not allow fluorescence detection of the derivatives, which is normally preferred to obtain better sensitivity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

Sampling and analytical methodology development for the determination of primary and secondary low molecular weight amines in ambient air

et al. 2008

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In this project, a sampling device and an analytical method have been developed to simultaneously analyse the most frequently found low molecular weight amines, including aliphatic, aromatic and alcohol amines. These amines are diethanolamine, ethanolamine, methylamine, isopropylamine, morpholine, dimethylamine, and aniline. A sampling device was developed using a 37 mm cassette with glass fibre filters impregnated with sulfuric acid. Immediately after sampling, the filter was transferred to vials containing a solution of dansyl chloride. Dansyl chloride was used for derivatisation because it forms aromatic sulfonamides that are fluorescent and easy to protonate for MS detection. The effect of using an internal standard made with the dansylated derivative of 1-(2-methoxyphenyl)piperazine (MOPIP) on the uncertainty and efficiency of the method was also evaluated. This internal standard was spiked directly onto filters. The coupling of HPLC/ESI-MS was used for the simultaneous analysis of all the derivatives. This method showed detection limits of about 0.03 microg mL(-1) to 0.3 microg mL(-1) of amine with an average expanded uncertainty of 3% to 6% depending on the amine. The methodology recoveries are close to 100% for all the amines, and the overall estimated expanded uncertainties vary between 10% and 13% depending on the amine. This new strategy will be useful in evaluating workplace air since a unique sampling system will be used, independent of the amine to be quantified.

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Section: Introductionmentioning

confidence: 99%

Sampling and analytical methodology development for the determination of primary and secondary low molecular weight amines in ambient air

et al. 2008

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“…Gas chromatographic methods with flame ionisation (FID), nitrogen-selective detectors (NPD) or mass detection (MS) and HPLC techniques with UV detection are common methods used for the analysis of amine derivatives [1,12,13,14]. Many of the derivatisation reactions described in the literature proceed in organic media or water and require long reaction times [13,14,15,16,17,18,19,20]. A method described by Lindahl et al [12] offers a sensitive and selective method for sampling of amines in the gas phase.…”

Section: Introductionmentioning

confidence: 99%

Development of a LC-MS/MS method for the analysis of volatile primary and secondary amines as NIT (naphthylisothiocyanate) derivatives

Claeson

Östin

Sunesson

2004

Analytical and Bioanalytical Chemistry

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High-performance liquid chromatography with mass spectrometric detection was used for the structure elucidation of eighteen primary and secondary amines and ammonia derivatised with naphthylisothiocyanate (NIT). A fragmentation scheme was established using reference compounds and the scheme was applied to real air samples from a tyre repair shop and from the air above a bacterial culture. The sampling was performed using a solid sorbent, XAD-2, impregnated with NIT, and the derivatives were extracted with acetonitrile and analysed with LC-MS/MS. A three-step process was developed for screening and identifying of volatile amines. The first step, selected reaction monitoring; SRM was applied in order to screen the samples for NIT derivatives. In the second step, a precursor ion scan gave the [M+H](+) ion, and in the third step a product ion scan gave the fragments needed for identification. The detection limits varied between 0.12 and 0.25 ng microL(-1) when screening for unknown derivatised amines. It was possible to separate and identify all the amines with the structural information obtained and the method proved to be general, sensitive and well suited for sampling and analysis of complex environmental samples.

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“…To date, a number of approaches including gas chromatography (GC) [8][9][10][11][12][13][14][15][16][17][18][19], high-performance liquid chromatography (HPLC) [10,[21][22][23][24][25][26][27][28] and capillary electrophoresis (CE) [24,[29][30][31][32] have been employed to determine low-molecular-mass amines. Because of its inherent advantage of high resolution, rapid separation, low cost and easy linkage with mass spectrometry (MS) to give unequivocal identification of compounds, capillary gas chromatography is a preferred method and often used.…”

Section: Introductionmentioning

confidence: 99%

Determination of aliphatic amines using N-succinimidyl benzoate as a new derivatization reagent in gas chromatography combined with solid-phase microextraction

Zhao

Cai

Jing

et al. 2003

Journal of Chromatography A

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Determination of secondary amines as highly fluorescent formamidines by high-performance liquid chromatography

Cited by 9 publications

References 10 publications

Sampling and analytical methodology development for the determination of primary and secondary low molecular weight amines in ambient air

Sampling and analytical methodology development for the determination of primary and secondary low molecular weight amines in ambient air

Development of a LC-MS/MS method for the analysis of volatile primary and secondary amines as NIT (naphthylisothiocyanate) derivatives

Determination of aliphatic amines using N-succinimidyl benzoate as a new derivatization reagent in gas chromatography combined with solid-phase microextraction

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