Determination of Selected Amino Acids in Serum of Patients with Liver Disease

Kanďár, Roman; Drábková, Petra; Toiflová, Tereza; Cegan, Alexander

doi:10.17219/acem/63751

Cited by 4 publications

(2 citation statements)

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“…The decrease in AAs with age was also found in our previous study for glycine and phenylalanine (Kanďár, Drábkova, Toiflová, et al, 2016). On the contrary, no effect of age on phenylalanine and tyrosine concentration was found in another study (Kanďár et al, 2009).…”

Section: Discussionsupporting

confidence: 75%

“…These findings correspond with the literature. The significantly higher plasma concentrations in men were also found in another study, although the number of significantly different AAs was higher (13 of 23 AAs tested) (Kanďár, Drábkova, Toiflová, et al, 2016). Similarly, higher levels in men (except glycine and serine which were higher in women) were found in another study (Mittelstrass et al, 2011).…”

Section: Discussionsupporting

confidence: 58%

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Dried blood spot as an alternative sample for screening of fatty acid, amino acid, and keto acid metabolism in humans

Laštovičková

Kopčil

Kanďár

2022

Biomedical Chromatography

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Dried blood spot (DBS) is a simple and noninvasive sample collection technique allowing self‐collection at home. It can be used as an alternative sample for the screening of metabolism in humans because changes in the levels of some fatty acids (FAs), amino acids (AAs), and keto acids (KAs) can be associated with metabolic disorders (e.g., diabetes mellitus). In this study, we optimized three different methods that are sensitive enough for the determination of the aforementioned analytes from a small volume of biological material in DBS. A total of 20 AAs, 5 KAs, and 24 FAs were determined. This sampling technique was applied to prepare samples from 60 individuals by a finger prick. The samples were analyzed using chromatographic methods, and acquired data were statistically evaluated. Even though most analytes were higher in men, only five AAs, three KAs, and eight FAs showed significant gender dependency (α = 0.05). Asparagine, serine, and α‐ and γ‐linolenic acids showed significant age dependency (α = 0.05). Most statistically significant correlations were positive and were found within one category. This work shows that because of many benefits, the DBS sample could be a good alternative to whole blood sample collection for the screening of metabolism in humans, in general, or in individualized medicine. The chromatographic methods can be used in future research, for example, to set the reference range or plasma‐correction factors (various aspects such as age or gender should be considered).

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Section: Discussionsupporting

confidence: 75%

Section: Discussionsupporting

confidence: 58%