Determination of solifenacin succinate in human plasma and pharmaceutical formulations using HPLC-UV

Abstract

An accurate, rapid and precise high performance liquid chromatographic coupled with ultraviolet detector method has been developed for the determination of solifenacin succinate in pharmaceutical formulation and human plasma. Quantitative analysis of solifenacin succinate was performed using a C18 column at wavelength at 210 nm. The mobile phase consisted of 10 mM ammonium formate buffer (pH 4.0), acetonitrile and methanol (52.5:32.5:12.5 v/v/v). The method showed linearity in the concentration range of 3-60 μg/mL and the correlation coefficient was 0.9998 and 0.9994 for the standard and human plasma solutions, respectively. The proposed method was within acceptable limits in terms of accuracy, precision, selectivity, linearity, sensitivity and recovery parameters. This method is readily applicable for the determination of solifenacin succinate in human plasma and pharmaceutical formulations.

Green micelle and complex inclusion enhance synchronous spectrofluorimetric quantification of a novel analgesic combination: Tramadol and celecoxib in tablet dosage form and spiked human plasma

“…[8] TRM has been previously analyzed using different techniques either alone or with others in different matrices. [9][10][11][12][13][14][15][16][17][18][19][20][21] Celecoxib (CLX; Figure 1b) is prescribed for the cure of pain, inflammation and the primary symptoms of many diseases and disorders, osteoarthritis among them, which ranks among the top causes of disability globally. [22] Various techniques have been published during the past years for CLX analysis such as spectrofluorimetry, [7,23] reverse phase high performance liquid chromatography (RP-HPLC), selective molecularly imprinted poly(vinylidene fluoride)…”

“…[ 8 ] TRM has been previously analyzed using different techniques either alone or with others in different matrices. [ 9–21 ]…”

Determination of Solifenacin Succinate in Pure and Pharmaceutical Dosage forms by Spectrophotometry

“…The literature survey has revealed that very few methods were developed for the quantification of the cited drug such as liquid chromatography-electro spray ionization tandem mass spectrometry [6,7] in human plasma, HPLC methods [8][9][10][11][12][13][14][15][16], high performance thin-layer chromatography [17,18], derivative spectrophotometry [19], derivative synchronous emission spectroscopy [20], UV spectroscopy [21] and spectrophotometry [22][23][24]. However, the reported methods are time consuming, require expensive chemicals, well equipped sophisticated instruments which are not easily accessible in all quality control laboratories, and the reported spectrophotometric methods suffer from such disadvantages as extraction, extended time required for the reaction to complete, narrow range of determination and lack of sensitivity.…”