Determination of Streptomycin Residue in Cucumber and Chinese Cabbage by High-Performance Liquid Chromatography with Postcolumn Derivatization and Fluorometric Detection

Abstract

A sensitive and accurate method was developed for the determination of streptomycin using HPLC followed by postcolumn derivatization and fluorometric detection. The analyte was extracted, using aqueous solution from cucumber and Chinese cabbage, by a two-step SPE procedure. The extraction, cleanup, and chromatography conditions were optimized, and the performance of the analysis method was evaluated. The conditions of chromatography were as follows: the separation was performed on a C18 column; the isocratic mobile phase consisted of acetonitrile and a mixed solution containing 10 mM sodium 1,2-naphthoquinone-4-sulfonate and 0.4 mM sodium 1-heptanesulfonate (25+75, v/v); and the flow rate was 1 mL/min. The fluorescence detector was set at an excitation wavelength of 263 nm and an emission wavelength of 435 nm. The calibration curve was linear over the range of 50-2000 ng/mL, with a correlation coefficient of 0.9995. The LOD and LOQ were 10 and 30 ng/g, respectively, in both cucumber and Chinese cabbage. The method was validated for selectivity, linearity, precision, and accuracy. The intraday and interday precision and accuracy were within 10%. The mean recoveries from spiked samples were more than 75%, with RSD lower than 10%.

Cytochrome c/Multi-walled Carbon Nanotubes Modified Glassy Carbon Electrode for the Detection of Streptomycin in Pharmaceutical Samples

“…Hence, its chemical synthesis and detection from biological fluids and pharmaceuticals are utmost importance in the field of chemistry. In the recent years, several techniques have been applied for STN detection in pharmaceuticals and biological fluids such as high-performance liquid chromatography (HPLC) 8 , ion exchange-high-performance thinlayer chromatography (HPTLC) 9,10 , liquid chromatography-tandem mass spectrometry (LC-MS/MS) 11 , and colorimetric visualization 12 . Although there have been significant improvements in these techniques, their application is limited due to exclusive equipment and substances, a complicated wash process and time consuming etc.…”

Poly-Eriochrome Black T Decorated with Nickel Hydroxide Nanoparticles Modified Electrode for the Electrochemical Detection of Streptomycin and Probing its Interaction with Double-stranded DNA

“…To date, numerous analytical methods have been developed to determine streptomycin. Some of them are based on chromatographic techniques such as high-performance liquid chromatography, 21,22 high-performance liquid chromatography-mass spectrometry, [23][24][25] liquid chromatography, 26,27 liquid chromatography-tandem mass spectrometry, 28,29 and ion pair liquid chromatography. 30 Despite their accuracy, the cost, long time of sample preparation, large amount of organic solvent, and the need for qualified labors constrained their practical applications.…”