Determination of Sucralose in Splenda and a Sugar-Free Beverage Using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection

Abstract

Sucralose is a chlorinated carbohydrate nonnutritive sweetener of food and beverage products. The determination of sucralose in food and beverages is important to ensure consistency in product quality. Sucralose was determined in two commercial products without sample preparation using highperformance anion-exchange (HPAE) chromatography coupled with pulsed amperometric detection (PAD). Sucralose was determined with a 10 min isocratic separation. To determine sucralose and other carbohydrates (e.g., dextrose) simultaneously, a gradient separation was developed. The linear range of electrochemical response extended over 3 orders of magnitude, from 0.01 (LOD) to 40 µM (16 µg/mL; 25 µL injection). High precision, high spike recovery, and method ruggedness were observed for both samples.

Kinetic spectrophotometric methods for the determination of artificial sweetener (sucralose) in tablets

“…The proposed methods are the first colorimetric methods used for SUC determination and are sensitive enough to enable the determination of lower amounts of the drug. Furthermore, the proposed methods have the advantage of using the spectrophotometer which is usually available in all quality control laboratories, rather than the previously reported methods that imply more sophisticated and expensive instruments that are usually unavailable 6–9. These advantages encourage the application of the proposed methods in routine analysis of SUC in quality control laboratories, as alternatives for the existing methods.…”

“…Detection of SUC and other carbohydrates is challenging because they lack a strong chromophore and, therefore, cannot be detected at low concentrations spectrophotometrically or chromatographically with UV detection. Literature survey reveals that there are only a few methods for the determination SUC either in food or in beverages including high performance liquid chromatography (HPLC),6, 7 HPTLC8 and capillary electrophoresis,9 but there is no reported method for the determination of SUC in tablet form. The aim of the present work is to develop kinetically based methods for the determination of SUC in tablets by measuring the absorbance at 610 nm after oxidation reaction with alkaline KMnO 4 or at 320 nm after reaction with cerium (IV) ammonium sulfate.…”

Re-Engineering an Artificial Sweetener: Transforming Sucralose Residuals in Water via Advanced Oxidation

“…There is also a hypothesis that sucralose is linked to gastrointestinal diseases in humans . However, drinking water is certainly not the major human exposure route for sucralose ingestion, as sucralose concentrations found in diet drinks are 1000–10 000 times the concentrations in drinking water …”

“…16 However, drinking water is certainly not the major human exposure route for sucralose ingestion, as sucralose concentrations found in diet drinks are 1000−10 000 times the concentrations in drinking water. 17 The manufacturing process of sucralose involves substitution of three hydroxyl groups on a sucrose molecule with chlorine. 18 During advanced oxidation, a common degradation pathway is addition of a hydroxyl group to carbons of the molecule.…”

Preparation of a Ligand Exchange Column for Carbohydrate Separation and Glucose Determination in Cell Culture Medium

“…[3][4][5] The sample preparation for HPLC is straightforward and rapid since non-derivatized carbohydrates can be injected directly. [5][6][7] A variety of detection methods can be used such as refractive index (RID), [8] ultraviolet, [9] amperometry, [10][11][12][13] and evaporative light scattering detection. [14,15] Carbohydrate separation using reversed phase columns has been reported.…”