Determination of sulfamerazine in aquatic products by molecularly imprinted capillary electrochromatography

Abstract

A molecularly imprinted monolith was prepared and evaluated for the special selective separation of sulfamerazine (SMR) by capillary electrochromatography (CEC). The single-step in situ polymerization method was applied through thermally immobilized vinyl groups of itaconic acid and a derivatization capillary column using SMR as the template. The monolith with optimal selectivity and permeability was performed at 45°C for 7 h according to the molar ratios of 1 : 4 : 10 (template/functional monomer/cross-linker). Under the optimized separation conditions of 75% acetonitrile in 20 mM phosphate buffer with pH 5.0, 15 kV applied voltage and 20°C column temperature, the imprinted monolith showed strong recognition ability for SMR and high column performance. Finally, the molecularly imprinted monolith coupled with the CEC method was successfully developed for the quantification of SMR in aquatic products, which was properly validated by a good linear relationship, recoveries and limit of detection. The coupling technique of the molecularly imprinted technology and CEC achieved pre-treatment enrichment and separation analysis in only one miniaturized chromatographic column.

Open Tubular Column Immobilized with Covalent Organic Frameworks for Rapid Separation of Small Molecular Compounds by Capillary Electrochromatography

“…The migration order of the sulfonamides was SM1, SDM, SM2, SMZ, SIZ and Esb3, as shown in Figure 8E. It was noticeable that the longest retention time for Esb3 was due to the strong polarity of the chlorine element (Cl) on Esb3, which has a strong π-π interaction with the TpBD COF in the stationary phase [36].…”

Recent developments of monolithic and open‐tubular capillary electrochromatography (2017–2019)

“…In recent years, molecular imprinting technique (MIT) has gained more and more attentions in different research areas . As one of the most ideal and promising methods to prepare functional materials, the main advantages of MIT are high specific recognition ability and physiochemical stability . Through MIT incorporate into a nonhydrolytic sol–gel process, Wang and co‐workers fabricated an organic–inorganic hybrid imprinted polymers monolithic column that can specific recognize the acephate and phosphamidon, attributing to the template molecule 4‐(dimethoxyphosphorothioylamino)butanoic acid.…”

β‐Cyclodextrin‐modified covalent organic framework as chiral stationary phase for the separation of amino acids and β‐blockers by capillary electrochromatography

“…Briefly, the bare capillary was pretreated. 42 The aminomodified column was obtained through filling with a methanolic solution of APTES (50%, v/v), heated and incubated in a 40 C water bath overnight, then rinsed with methanol to flush out the residuals, and dried by a nitrogen stream. Next, the prepolymerization solution of the synthesized MDI-β-CD-Tp and the ordered Pa-1 were poured into the modified capillary.…”