Determination of sulfonamides in livers using matrix solid‐phase dispersion extraction high‐performance liquid chromatography

Abstract

The matrix solid-phase dispersion (MSPD) was applied for extracting seven sulfonamides (SAs) in liver samples. The separation and determination were carried out by high-performance liquid chromatography. The analytes were derivated with fluorescamine and detected with fluorescence detector. The types of dispersion adsorbents for MSPD were examined and the highest recovery was obtained when the diatomaceous earth was used as the dispersion adsorbent and the mass ratio of dispersion adsorbent to sample was 3:1. The acetone was used as the elution solvent. Under the optimal conditions, the linear range for determining the SAs in liver samples was 5.0-1000.0 ng/g. The porcine, chicken and cattle liver samples were analyzed and the average recoveries of seven SAs were higher than 84.6%.

Rapid Screening Alpha-Glucosidase Inhibitors from Polygoni Vivipari Rhizoma by Multi-Step Matrix Solid-Phase Dispersion, Ultrafiltration and HPLC

“…According to previous reports, methanol was commonly used as the extract solvent because it is practical and has superior capacity for extracting components from herbal medicine [10,15]. Diatomaceous earth, which has been used in component extraction of PVR, was chosen as the dispersant based on its excellent dispersion capacity and low interference [16].…”

Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

“…it requires only small amounts of sample and solvents, it is fast, inexpensive and can be carried out under mild extraction conditions, at room temperature and atmospheric pressure), it is not always reported as more efficient than other techniques, and is less prone to complete automation, because it requires sample blending the sample blending in a mortar with a pestle, which can only be performed manually (Capriotti et al, ). Some examples of using this technique as the SP choice for bionalaytical studies are the following: isolation of ofloxacin, pefloxacin, norflorxacin, ciprofloxacin and enrofloxacin in chicken eggs and swine tissues using MIPs as selective MSPD sorbents (Yan, Qiao, & Row, ); isolation of cocaine, benzoylecgonine, codeine, morphine and 6‐monoacetylmorphine from human hair using alumina as a dispersing agent (Míguez‐Framil et al, ); and extraction of several sulfonamides in liver samples using diatomaceous earth as the dispersion adsorbent (Y. Zhang et al, ).…”

“…Some examples of using this technique as the SP choice for bionalaytical studies are the following: isolation of ofloxacin, pefloxacin, norflorxacin, ciprofloxacin and enrofloxacin in chicken eggs and swine tissues using MIPs as selective MSPD sorbents (Yan, Qiao, & Row, 2007); isolation of cocaine, benzoylecgonine, codeine, morphine and 6monoacetylmorphine from human hair using alumina as a dispersing agent (Míguez-Framil et al, 2010); and extraction of several sulfonamides in liver samples using diatomaceous earth as the dispersion adsorbent (Y. Zhang et al, 2012).…”

Analysis of trace levels of sulfonamides in fish tissue using micro-scale pipette tip-matrix solid-phase dispersion and fast liquid chromatography tandem mass spectrometry

“…Methods using HPLC or UPLC as an analytical technique have received much attention and many researchers have made use of these techniques in sulfonamides analysis (García-Galán et al, 2011). With LC methods, sulfonamide residues are determined either directly, after separation on a chromatographic column, using a UV detector or mass spectroscopy (MS) or indirectly after derivatization by fluorescence detection (Yu & Hu, 2012;Zhang et al, 2012).…”