Determination of Thailandepsin B in Rat Plasma with A LC–MS/MS Method

Cheng, Zhongzhe; Ding, Lian; Zhang, Yang; Chen, Guiying; Chen, Chang; Cheng, Yi-Qiang; Jiang, Hongliang

doi:10.4155/bio.14.264

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…Remarkably, the higher concentration of LC‐A was found in stomach and intestine, which is similar to LC‐B and LC‐C. Owing to the higher concentration of LC‐B and LC‐C in the stomach, more LC‐A could be obtained from LC‐B and LC‐C hydrolysis by gastric juice, Similarly, the high level of LC‐A was also observed in the intestine, which might be attributed to enzymatic hydrolysis and intestinal microflora biotransformation (Cheng et al, ).…”

Section: Resultsmentioning

confidence: 97%

Tissue distribution of capilliposide B, capilliposide C and their bioactive metabolite in mice using liquid ‐tandem mass spectrometry

Cheng

Zhou

et al. 2016

Biomedical Chromatography

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Lysimachia capillipes Hemsl (Primulaceae), a folk medicinal plant in China, showed significant anti-tumor activities in vivo and in vitro. Capilliposide B (LC-B) and capilliposide C (LC-C) are the main bioactive components in this plant. To explore their tissue distribution, a reliable bioanalytical method for the quantification of LC-B, LC-C and their bioactive metabolite, capilliposide A (LC-A), in mouse tissues was developed and validated. In this study, the tissue distribution profiles of the three compounds were examined after intravenous administration of pure LC-B and oral administration of total saponins of L. capillipes Hemsl extract (LCE) for 10 days. Method validation was conducted over the curve range 10.0-5000 ng/mL for all three analytes in various tissue homogenates. The relative standard deviation of intra-day and inter-day precision of the QC samples was <14.7%, and the accuracy ranged from 85.9 to 114.0%. The results indicated that LC-B was rapidly and widely distributed throughout the whole body except for muscle following intravenous administration of LC-B. In addition, LC-A was only in liver, intestine, lung and stomach. After oral administration of LCE, LC-B and LC-C were distributed into various tissues. The highest levels were observed in stomach and intestine.

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Section: Resultsmentioning

confidence: 97%

Tissue distribution of capilliposide B, capilliposide C and their bioactive metabolite in mice using liquid ‐tandem mass spectrometry

Cheng

Zhou

et al. 2016

Biomedical Chromatography

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“…Levodopa and MD01 are easily oxidized in solutions and biological matrices because of the phenol moiety; it was therefore necessary to stabilize the analytes. Antioxidants such as ascorbic acid (vitamin C) and sodium metabisulfate, adjusting pH with formic acid, hydrochloric acid or phosphoric acid, and temperature control are commonly used in bioanalysis of unstable small molecules (Chen and Hsieh, ; Li et al ., ; Cheng et al ., ). It is interesting to note that ascorbic acid was previously used as a stabilizer in the extraction process of MP (Misra and Wagner, ).…”

Section: Resultsmentioning

confidence: 97%

Development and validation of an LC‐MS/MS method for simultaneous quantification of levodopa and MD01 in rat plasma and its application to a pharmacokinetic study of mucuna pruriens extract

Yang

Zhang

Deng

et al. 2016

Biomedical Chromatography

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Mucuna pruriens, an ancient Indian herbal medicine containing levodopa, is widely used for Parkinson's disease. In order to simultaneously determine levodopa and 1,1-dimethyl-3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (MD01) in rat plasma, an improved LC-MS/MS method was developed and validated for a pharmacokinetic study in rats orally administered levodopa or Mucuna pruriens extract (MPE). Elimination of matrix effect and improvement of extraction recovery were achieved through systematic optimization of reversed-phase and hydrophilic interaction chromatographic conditions together with sample clean-up procedures. A satisfactory chromatographic performance was obtained with a Thermo Aquasil C18 column (50 × 2.1 mm, 3 µm) using acetonitrile and water containing 0.2% formic acid as mobile phases. Futhermore, sodium metabisulfite and formic acid were used as stabilizers in neat solutions as well as rat plasma. The method was validated in a dynamic range of 20.0-10,000 ng/mL for levodopa and MD01; the intra- and inter-day precision and accuracy were acceptable. The method was successfully utilized to determine the levodopa level in plasma samples of rats administered levodopa or MPE. Pharmacokinetic results showed that an increase in the AUC of levodopa was observed in rats following oral administration of multiple doses of MPE. Copyright © 2016 John Wiley & Sons, Ltd.

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“…Thus, the metabolism of LC-B and LC-C was not NADPH-dependent in MLM. Recently, we employed a typical esterase inhibitor (dichlorvos) to prevent esterolysis of LC-B and LC-C in rat plasma [9,13]. In the current work, dichlorvos (1 mM) was added to the same incubation system, including MLM.…”

Section: Resultsmentioning

confidence: 99%

Metabolic Stability and Metabolite Characterization of Capilliposide B and Capilliposide C by LC–QTRAP–MS/MS

Cheng

Zhou

et al. 2018

Pharmaceutics

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Capilliposide B (LC-B) and Capilliposide C (LC-C), two new triterpenoid saponins extracted from Lysimachia capillipes Hemsl, exhibit potential anticancer activity both in vitro and in vivo. However, their metabolic process remains unclear. In this study, the metabolic stability of LC-B, LC-C, and Capilliposide A (LC-A, a bioactive metabolite of LC-B and LC-C) was investigated in human, rat, and mouse liver microsomes, respectively. Thereafter, their metabolites were identified and characterized after oral administration in mice. As a result, species difference was found in the metabolic stability of LC-B and LC-C. All three compounds of interest were stable in human and rat liver microsomes, but LC-B and LC-C significantly degraded in mouse liver microsomes. The metabolic instability of LC-B and LC-C was mainly caused by esterolysis. Moreover, 19 metabolites were identified and characterized in mouse biological matrices. LC-B and LC-C mainly underwent deglycosylation and esterolysis, accompanied by dehydration, dehydrogenation, and hydroxylation as minor metabolic reactions. Finally, the metabolic pathway of LC-B and LC-C in mice was proposed. Our results updated the preclinical metabolism and disposition process of LC-B and LC-C, which provided additional information for better understanding efficacy and safety.

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Determination of Thailandepsin B in Rat Plasma with A LC–MS/MS Method

Abstract: A reliable LC-MS/MS method was developed and validated for the quantification of TDP-B and was successfully applied to a rat pharmacokinetic study.

Cited by 4 publications

References 15 publications

Tissue distribution of capilliposide B, capilliposide C and their bioactive metabolite in mice using liquid ‐tandem mass spectrometry

Tissue distribution of capilliposide B, capilliposide C and their bioactive metabolite in mice using liquid ‐tandem mass spectrometry

Development and validation of an LC‐MS/MS method for simultaneous quantification of levodopa and MD01 in rat plasma and its application to a pharmacokinetic study of mucuna pruriens extract

Metabolic Stability and Metabolite Characterization of Capilliposide B and Capilliposide C by LC–QTRAP–MS/MS

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