Determination of the authenticity of vanilla extracts by stable isotope ratio analysis and component analysis by HPLC

Development and validation of an RP‐HPLC method for quantitative determination of vanillin and related phenolic compounds in Vanilla planifolia

“…The quality evaluation of vanilla extracts is achieved by numerous methods such as TLC [6 -8], high performance TLC (HPTLC) [9 -12], GC [13], isotope ratio MS [14,15], CE [16], MEKC [16,17], and HPLC [18 -27]. HPLC is a widely exploited tool for the quality control of vanilla flavor due to its high sensitivity and accuracy.…”

“…Reports are available on different HPLC methods used for separation and identification of phenolic compounds in vanilla extract. Lamprecht et al [14] have separated 16 phenolic compounds but quantified four phenolic compounds, while Pyell et al [29] have separated 12 phenolic compounds and quantified six phenolic compounds. Recently, Boyce et al [17] have separated 11 phenolic compounds and quantified five phenolic compounds in Vanilla species.…”

Rapid sample screening method for authenticity controlling vanilla flavors using a CE microchip approach with electrochemical detection

“…However, the presence of VAN in vanilla-related samples is not unequivocal proof of their authenticity because of adulteration of vanilla extracts with synthetic, lignin-derived VAN is a major problem in commercial market product [20]. In addition, the high demands and the high price for authentic vanilla extracts leads to numerous efforts of blending and adulteration verifying their authenticity by MS or by multianalytes detection in vanilla extract profiles using GC and HPLC [21][22][23][24]. MAL (E-636) and EMA (E-637) are used primarily as flavor enhancers [25].…”

RP‐HPTLC densitometric determination and validation of vanillin and related phenolic compounds in accelerated solvent extract of Vanilla planifolia*

“…Various approaches have been applied for quality control of vanilla extracts such as TLC [15,16], HPTLC [6, 17 -20], GC [21], isotope ratio MS [22,23], CE [24], MEKC [25] and HPLC [26 -32]. Among these, HPLC has since been remained the most widely exploited tool due to its high sensitivity and accuracy.…”