Determination of the concentration of low molecular fraction of Сandida Albicans proteins by ELISA method at subcutaneous introduction in candidiasis therapy

Abstract

The purpose of this work was to determine the C. albicans protein concentration at the subcutaneous introduction. The therapeutic effect of C. albicans proteins has been studied in white mice. Animals were injected intraperitoneally with a suspension of C. albicans fungus. After five days and repeatedly, after 14 days, mice were infected subcutaneously with proteins of Candida cells of volume 0.2 mL. Fourteen days after each injection, the determination of the protective functions of the animal body has been carried out by the titer of specific C. albicans antibodies. According to the data obtained during studies on the treatment of candidiasis, it was found that in the subcutaneous administration after the first and second injection with a concentration of low molecular weight fraction of the C. albicans protein of 1, 2, 3, 4, and 5 mg/mL antibodies titers have doubled, indicating a lack of activation of the body's protective functions. Proteins of low molecular weight fraction of C. albicans with concentrations 1, 2, 3, 4, and 5 mg/mL do not activate the body's defence mechanisms.