The purpose of this work is the study of antibody titers of low molecular weight proteins fractions less than ten kDa with C. Albicans protein concentrations of 1, 2, 3 and 4 mg/ml in the prevention of candidiasis. A low molecular fraction of C. Albicans proteins in concentration 1, 2, 3, 4 and 5 mg/ml were examined in white mice. The mice were intramuscularly injected into the upper part of the right hind paw 0.2 ml of the test fractions with the investigated protein concentrations. After 14 days, 0.2 ml of test fractions were injected again into the upper part of the left hind paw. Animals in the control group were administered saline. After one month for one group and after three months for the second group of experimental animals after the second injection, intraperitoneal infection of the animals was carried out. For this purpose, a suspension of C. Albicans fungi was used in the amount of 20 million cells in a volume of 1 ml. After 14 days, determination of the protective functions of the animal body by the titer of specific C. Albicans antibodies has been performed during enzyme-linked immunoassay. Studies have shown that the low molecular weight fraction of C. Albicans fungi cells antigens with protein concentrations of 1, 2, 3 and 4 mg/ml at a double intramuscular injection of 0.2 ml does not provide activation of immune mechanisms. The low molecular weight fraction of C. Albicans proteins does not activate the body's defence mechanisms.

The objective of this work was to determine the C. albicans fungus protein concentration at intramuscular introduction in therapy of candidiasis. Therapeutic effect of C. albicans fungus proteins in concentrations 1, 2, 3, 4 and 5mg/mL has been examined in white mice. Animals were infected intraperitoneally with suspension of C. albicans fungus strain CCM 335-867 in the amount of 20 million cells per 1mL volume. After 5 days and repeatedly after 14 days mice were injected intramuscularly in the upper back hind paw with proteins of Candida fungus cells of volume 0.2mL. 14 days after each injection, the determination of the protective functions of the animal body has been carried out by the titer of specific C. albicans antibodies during immunoassay. According to the data obtained during studies on the treatment of candidiasis, it has been found that in the intramuscular route of administration after the first injection with C. albicans protein concentration of 3 mg/mL antibody titers increased 2 times, and after the second - 8 times. Proteins of C. albicans fungus at a concentration of 3 mg/mL activate the body's defence mechanisms.

No abstract

The purpose of this work was to determine the C. albicans protein concentration at the subcutaneous introduction. The therapeutic  effect of C. albicans proteins has been studied in white mice.  Animals were injected  intraperitoneally with a suspension of C. albicans fungus.  After five days and repeatedly, after 14 days, mice were infected subcutaneously  with proteins of Candida cells of volume 0.2 mL. Fourteen days after each injection, the determination of the protective functions of the animal body has been carried out by the titer of specific C. albicans antibodies. According to the data obtained during studies on the treatment of candidiasis, it was found that in the subcutaneous administration after the first and second injection with a concentration of low molecular weight fraction of the C. albicans protein of 1, 2, 3, 4, and 5 mg/mL antibodies titers have doubled, indicating a lack of activation of the body's protective functions. Proteins of low molecular weight fraction of C. albicans with concentrations 1, 2, 3, 4, and 5 mg/mL do not activate the body's defence mechanisms.