Determination of the<i>aerolysin</i>gene in<i>Aeromonas hydrophila</i>using the polymerase chain reaction (pcr) technique

Christy, George C.; Kusdawarti, R.; Handijatno, Didik

doi:10.1088/1755-1315/236/1/012097

Cited by 3 publications

(3 citation statements)

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“…The aer-A gene was identified using the primers forward 5'-CAAGAACAAGTTCAAGTGGCCA-3' and reverse 5'-ACGAAGGTGTGGTTCCAGT-3' (GoTaq ® Green DNA polymerase, Promega Corp, USA). A total of 12.5 μL of polymerase chain reaction (PCR) buffer mixture was prepared that consisted of 2.5 μL of magnesium chloride (25 mM), 0.5 μL of deoxynucleoside triphosphate mixture (200 μM), 2.5 μL of the forward primer (12 μM), 2.5 μL of the reverse primer (12 μM), 12.5 mM (GoTaq ® Green DNA polymerase, Promega Corp), and 3 μL of DNA template [11].…”

Section: Aerolysin Gene Identificationmentioning

confidence: 99%

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Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia

et al. 2022

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Background and Aim: Motile Aeromonas septicemia is a crucial disease in freshwater fish. Aeromonas hydrophila is a disease agent associated with sporadic fish mortality, food safety, and public health. This study aimed to estimate the prevalence and the presence of the aerolysin gene and antimicrobial resistance profile of A. hydrophila isolated from milkfish in Gresik, Indonesia. Materials and Methods: A total of 153 milkfish gill samples were collected from 16 locations in Gresik and then cultured and identified using biochemical tests. The aerolysin gene was investigated using a polymerase chain reaction, and antimicrobial resistance profiles of the recovered isolates were investigated. Results: Of the 153 examined samples, 35 (22.9%) were confirmed positive for A. hydrophila and 22 (62.9%) presented the aerolysin gene. The recovered isolates were resistant to the following antibiotics: Amoxicillin (62.9%), tetracycline (60%), streptomycin (54.3%), cefotaxime (51.4%), gentamycin (31.4%), kanamycin (28.6%), erythromycin (25.7%), chloramphenicol (20%), and trimethoprim (14.3%). Meanwhile, only ciprofloxacin, nalidixic acid, and imipenem were indicated as susceptible. Conclusion: The presence of the aerolysin gene is vital in determining the virulence of A. hydrophila. The study results indicated a high aerolysin gene prevalence. In addition, this study emphasized antibiotic use monitoring, food safety improvement, and negative impact reduction on human health and the environment.

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Section: Aerolysin Gene Identificationmentioning

confidence: 99%

“…The amplification results were then visualized with agarose gel electrophoresis of 0.8% buffer, tris-acetate-ethylenediaminetetraacetic acid, and 100 bp marker (GoTaq ® Green DNA polymerase, Promega Corp). The aer-A gene-positive samples were revealed at an amplicon length of 309 bp [11].…”

Section: Aerolysin Gene Identificationmentioning

confidence: 99%

Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia

et al. 2022

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“…Bakteri A. hydrophilla menyebabkan salah satu penyakit yaitu Motile Aeromonas Septicemia (MAS), penyakit ini dapat mengakibatkan angka kematian yang tinggi pada ikan yaitu 80-100% pada waktu yang singkat 1-2 minggu. (Christy et al, 2019). Serangan bakteri A. hydrophilla bersifat patogen pada ikan nila, sehingga gejala klinis yang ditimbulkan yaitu bercak merah pada permukaan tubuh ikan (haemoraghic), mata ikan menonjol (exopthalmia) dan perut buncit serta warna tubuh ikan menjadi gelap (dropsy) ujung sirip terputus-putus serta pada bagian sirip punggung terdapat bintik putih dan geripis (Rosidah et al, 2018).…”

Section: Pendahuluanunclassified

AKTIVITAS KITOSAN LIMBAH CANGKANG KERANG BULU (Anadara antiquata) TERHADAP Aeromonas hydrophila PADA IKAN NILA (Oreochromis niloticus)

Tuti Aulia,

Syafrina Sari Lubis,

Diannita Harahap

2022

kenanga

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Aquaculture in Indonesia is carried out in freshwater, brackish and marine waters with limited production of several fish species such as tilapia. Although tilapia is easy to adapt to the environment, it can be infected with Aeromonas hydrophila bacteria. One of the efforts to treat bacteria is to use chitosan which is a chitin derivative that can be developed as an antimicrobial candidate because it contains lysozyme enzymes and aminopolysaccharide groups that can inhibit microbial growth. The purpose of this study was to determine the activity of chitosan from feather shell waste (Anadara antiquata) in inhibiting Aeromonas hydrophila in vitro and in vivo in tilapia. The methods used for the manufacture of chitosan include deproteination, demineralization, depigmentation, and deacetylation. In vitro testing was conducted to test the antibacterial activity using the disc diffusion method (Kirby-Bauer test). In vivo testing was carried out by the immersion method. The results showed that the chitosan yield was 77.2%. The most effective concentration of chitosan antibacterial activity against Aeromonas hydrophila was at 7% with an inhibition zone value of 6.85 mm. The results of data analysis showed that there was a significant difference between the test treatments. The in vivo test on tilapia showed that the highest percentage was found in the optimal concentration treatment (7% chitosan) with a value of 68.74%. The results of data analysis showed that there was no significant difference between optimal concentration treatment and positive control.

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Motile Aeromonas Septicemia (MAS) Disease Resistance Test by Aeromonas hydrophila on Triploid Striped Catfish (Pangasianodon hypopthalmus)

Hartami,

Ayuzar,

Salamah

et al. 2024

JAFH

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Motile Aeromonas Septicemia (MAS) disease caused by Aeromonas hydrophila is a common pathogen that attacks freshwater fish commodities, including triploid as the new strain of striped catfish. This study aimed to test the resistance of triploid striped catfish to MAS infection before being released to farmers as a new commodity to increase national striped catfish production. The test was carried out for two months at the Sukamandi Fish Breeding Research Institute, Subang by injecting A. hydrophila into triploid and diploid striped catfish. The challenge test was carried out by injecting the cultured A. hydrophila with a density of 107 cfu.mL-1 as much as 0.1 mL.ind-1 at fish intramuscularly in all treatments, while in the Control (-) treatment, 0.1 mL of PBS solution was injected.tail-1. The test striped catfish used for each treatment were 10 individuals measuring 9.23 – 9.65 cm, and weighing 7.1 – 9.23 g. Survival parameter data and blood description were analyzed quantitatively using Microsoft Excel 2010 and SPSS version 16.0 software with analysis of variance (ANOVA) at a 95% confidence interval. The results of re-characterization with biochemical tests showed that A. hydrophila were gram-negative, oxidase and catalase positive, motile, could fermentatively convert sugar, and could grow in blood media. The survival of triploid striped catfish after the test was 95%, total erythrocytes on day 7, and total leukocytes on the first day were significantly different from diploid striped catfish. Thus, it can be concluded that striped triploid striped catfish are more resistant to infection with A. hydrophila than diploid.

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Determination of theaerolysingene inAeromonas hydrophilausing the polymerase chain reaction (pcr) technique

Cited by 3 publications

References 5 publications

Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia

Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia

AKTIVITAS KITOSAN LIMBAH CANGKANG KERANG BULU (Anadara antiquata) TERHADAP Aeromonas hydrophila PADA IKAN NILA (Oreochromis niloticus)

Motile Aeromonas Septicemia (MAS) Disease Resistance Test by Aeromonas hydrophila on Triploid Striped Catfish (Pangasianodon hypopthalmus)

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