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Food borne disease merupakan isu bagi dunia kesehatan karena masih belum bisa dikendalikan secara tuntas. Daging merupakan salah satu agen tempat berkembangnya mikroba infeksius, cemaran kimia dan fisik yang memungkinkan timbulnya food poisoning. Penanganan masalah food borne disease melalui penatalaksanaan penyediaan pemotongan melalui Rumah Potong Hewan (RPH) menjadi salah satu kunci untuk mengendalikan bahan pangan. Kualitas daging menjadi prioritas penting dalam menjamin mutu pangan. Penelitian menggunakan kajian cross sectional study dengan metode sampling purposive. Pengujian sampel di wilayah Kecamatan Banyuwangi, Genteng dan Kalibaru menjadi subjek utama dimana terdapat kios daging yang mensuplai kebutuhan konsumen sehingga akan diketahui kualitas daging yang dipotong melalui RPH. Penelitian ini menunjukkan hasil uji organoleptis warna 37% kelainan, bau normal, tekstur konsistensi keras 0,05%, uji pH 50% diatas ambang normal, uji Eber 45% positif mengalami awal kebusukan. Semua sampel daging negative Salmonella spp dan 50% melebihi ambang batas Standart Nasional Indonesia cemaran Escherichia coli.

Background and Aim: Stress can cause physiological and biological disorders in the body. On the other hand, antioxidants from vitamins and minerals are effective for stress treatment. Therefore, this study aimed to evaluate the effect of the administration of Vitamins C and E on serum superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPx), testosterone, and cortisol activity in albino rats with chronic variable stress (CVS). Materials and Methods: Twenty albino rats were randomly assigned into four treatment groups: C was administered normal saline; T1 was administered Vitamins C and E; T2 was only induced CVS; and T3 was induced CVS followed by Vitamins C and E administration. All treatments were applied for 4 weeks, respectively. Furthermore, 5 mL of blood samples were collected intracardially. Body weight data were collected for the initial and final weights. From serum samples, SOD, GPx, and CAT were measured using the enzymol method; MDA was measured using the high-performance liquid chromatography method; and testosterone and cortisol were measured using the enzyme-linked immunosorbent assay method. All variables were analyzed statistically using analysis of variance followed by the Duncan test (p<0.05). Results: Our findings showed that the T1 and T3 groups significantly decreased (p<0.001) compared to T2 in the following parameters: SOD, MDA, GPx, and cortisol. Meanwhile, CAT and testosterone levels in the T1 and T3 groups were significantly increased (p<0.001) compared to the T2 group. In addition, the weight gain in T1 and T3 groups was significantly increased (p<0.001) compared to T2 group. Conclusion: It can be concluded that the administration of Vitamins C and E had a significant effect to alleviate SOD, MDA, GPx, and cortisol and to improve the testosterone level in albino rats with CVS.

Tujuan penelitian ini untuk mengetahui efektivitas ekstrak etanol kayu secang (Caesalpinia sappan L.) secara topikal dapat mempercepat kepadatan kolagen pada penyembuhan luka insisi tikus putih (Rattus norvegicus). Sebanyak 20 ekor tikus jantan dibagi secara acak menjadi lima kelompok, yakni: kontrol negatif (K-) yang diberi basis salep, kontrol positif (K+) yang diberi salep povidone iodine 10%, kelompok perlakuan (P1; P2; P3) yang diberi salep ekstrak kayu secang 6.5%; 15%; 30%. Perlakuan diberikan pada daerah luka secara topikal sekali sehari selama 14 hari. Data hasil skoring histopatologi diuji dengan uji Kruskal-Wallis dan Mann-Whitney. Kepadatan kolagen luka insisi kelompok P1 berbeda signifikan dengan kelompok P2, P3, K-, dan K+, dan tidak ada kelompok yang berbeda secara signifikan selain itu. Dapat disimpulkan bahwa peningkatan rata-rata kepadatan kolagen pada kelompok P1 adalah dosis optimal.

Background and Aim: Motile Aeromonas septicemia is a crucial disease in freshwater fish. Aeromonas hydrophila is a disease agent associated with sporadic fish mortality, food safety, and public health. This study aimed to estimate the prevalence and the presence of the aerolysin gene and antimicrobial resistance profile of A. hydrophila isolated from milkfish in Gresik, Indonesia. Materials and Methods: A total of 153 milkfish gill samples were collected from 16 locations in Gresik and then cultured and identified using biochemical tests. The aerolysin gene was investigated using a polymerase chain reaction, and antimicrobial resistance profiles of the recovered isolates were investigated. Results: Of the 153 examined samples, 35 (22.9%) were confirmed positive for A. hydrophila and 22 (62.9%) presented the aerolysin gene. The recovered isolates were resistant to the following antibiotics: Amoxicillin (62.9%), tetracycline (60%), streptomycin (54.3%), cefotaxime (51.4%), gentamycin (31.4%), kanamycin (28.6%), erythromycin (25.7%), chloramphenicol (20%), and trimethoprim (14.3%). Meanwhile, only ciprofloxacin, nalidixic acid, and imipenem were indicated as susceptible. Conclusion: The presence of the aerolysin gene is vital in determining the virulence of A. hydrophila. The study results indicated a high aerolysin gene prevalence. In addition, this study emphasized antibiotic use monitoring, food safety improvement, and negative impact reduction on human health and the environment.