Determination of the Molecular Mass of New l-Fucose-Containing Glycosaminoglycan and Its Distribution by High-Performance Gel-Permeation Chromatography with Laser Light-Scattering Detection

Tsukamoto, Tsuyoshi; Hattori, Motonobu; Sakabe, Michio; Haginaka, Jun

doi:10.2116/analsci.17.555

Cited by 17 publications

(7 citation statements)

References 20 publications

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“…The average molecular weight of SCP-1 was estimated to be 15.63 kDa on the basis of calibration with standard d -series dextrans. M w / M n was used to determine the width of the molecular weight distribution, which represents the dispersibility and homogeneity of a polymer . As a result, the M w / M n for SCP-1 was 1.695 estimated by GPC software version B01.01 (Agilent, USA).…”

Section: Resultsmentioning

confidence: 99%

Structural Characterization and Immunostimulatory Activity of a Homogeneous Polysaccharide from Sinonovacula constricta

Yuan

Zhao

Cha

et al. 2015

J. Agric. Food Chem.

124

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Sinonovacula constricta has been widely used as a health food and medicine in China, Japan, and Korea. In the present study, a water-soluble polysaccharide fraction (SCP-1) was prepared from S. constricta by enzyme-assisted extraction and purification of chromatography with DEAE-52 cellulose anion-exchange column and Sephadex G-100 size exclusion column. On the basis of the analytical results of high-performance liquid chromatography, Fourier transform-infrared spectroscopy, methylation analysis, and NMR spectroscopy, SCP-1 was found to have an average molecular weight of 15.63 kDa and a linear backbone of (1→4)-linked α-D-Glcp residue with one branch, α-D-Glcp, attached to the main chain by a (1→6) glycosidic bond at every five α-D-Glcp units. Furthermore, it was found that SCP-1 could significantly increase the viability of macrophages, enhance the capability of macrophage phagocytosis, increase the activity of acid phosphatase, and promote the production of nitric oxide, mouse tumor necrosis factor (TNF)-α, mouse interferon (IFN)-γ, and mouse interleukin (IL)-1β. The results suggest that SCP-1 possesses potent immunomodulating effect and may be explored as a potential biological response modifier.

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Section: Resultsmentioning

confidence: 99%

Structural Characterization and Immunostimulatory Activity of a Homogeneous Polysaccharide from Sinonovacula constricta

Yuan

Zhao

Cha

et al. 2015

J. Agric. Food Chem.

124

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“…The molecular weight distribution ( M w / M n ) is used to measure the width of molecular weight distribution, which represents the homogeneity and dispersibility of the polymer. The distribution data indicates that the natural polysaccharides are homogeneous [23]. …”

Section: Resultsmentioning

confidence: 99%

“…The purity of preparation was assayed by high-performance gel permeation chromatography (HPGPC) using a Agilent technologies1200 series (Agilent Co., USA) apparatus with RID (G1362A) and DAD (G1315D) detectors, equipped with a Shodex OH-pak SB-804 HQ column (8 mm × 300 mm). Chromatographic conditions and procedures were performed according to the previous method [23]. …”

Section: Methodsmentioning

confidence: 99%

“…Chromatographic conditions and procedures were performed according to the previous method [23]. For molecular weight estimation, the columns were calibrated by standard D -series Dextrans ( D -0, 2, 3, 4, 5, 6, 7, 8 and 2000), a FuCS from the sea cucumber T. ananas with known relative molecular weight ( M w 65,820 Da) for the FuCS and sulfated fucan, a dextran from Leuconostoc spp.…”

Section: Methodsmentioning

confidence: 99%

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Comparison of Physicochemical Characteristics and Anticoagulant Activities of Polysaccharides from Three Sea Cucumbers

et al. 2013

Marine Drugs

141

111

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In order to search for sulfated polysaccharides in different invertebrate connective tissues and to examine their biological activities, we have isolated three types of polysaccharides from the body wall of the three sea cucumbers Holothuria edulis, Apostichopus japonicas and Holothuria nobilis. The physicochemical properties and anticoagulant activities of these polysaccharides were examined and compared. The chemical composition analysis and nuclear magnetic resonance (NMR) analysis indicate that two types of polysaccharides, sulfated fucan and fucosylated chondroitin sulfate (FuCS), were found in all of the three species and in addition a neutral glycan was observed in H. edulis. The neutral α-glucan was firstly obtained from sea cucumber. The same type of polysaccharides from different species of sea cucumbers have similar physicochemical properties and anticoagulant activities, but those of different types of glycans are significantly different, possibly due to their different monosaccharide compositions, electric charges and average molecular weights. The FuCSs have stronger anticoagulant activities than the sulfated fucans, although the molecular sizes of the FuCSs are lower than those of the sulfated fucans, whereas the neutral glucan has no activity, as expected from the absence of sulfate. Thus, anticoagulant activities of the different type of polysaccharides are likely to relate to monosaccharide composition and sulfate content. Preliminary analysis suggests that the sulfation patterns of the FuCSs may result in the difference in anticoagulant activities. Our data could help elucidate the structure-activity relationship of the sea cucumber polysaccharides.

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“…The purity of preparation was assayed by high-performance gel permeation chromatography (HPGPC) using a Agilent technologies1200 series (Agilent Co., Santa Clara, CA, USA) apparatus with RID (G1362A) and DAD (G1315D) detectors, equipped with a Shodex OH-pak SB-804 HQ column (8 mm × 300 mm). Chromatographic conditions and procedures were performed according to the previous method [ 19 , 36 ].…”

Section: Methodsmentioning

confidence: 99%

Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber

Zhao

et al. 2015

Marine Drugs

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Sulfated fucans, the complex polysaccharides, exhibit various biological activities. Herein, we purified two fucans from the sea cucumbers Holothuria edulis and Ludwigothurea grisea. Their structures were verified by means of HPGPC, FT-IR, GC–MS and NMR. As a result, a novel structural motif for this type of polymers is reported. The fucans have a unique structure composed of a central core of regular (1→2) and (1→3)-linked tetrasaccharide repeating units. Approximately 50% of the units from L. grisea (100% for H. edulis fucan) contain sides of oligosaccharides formed by nonsulfated fucose units linked to the O-4 position of the central core. Anticoagulant activity assays indicate that the sea cucumber fucans strongly inhibit human blood clotting through the intrinsic pathways of the coagulation cascade. Moreover, the mechanism of anticoagulant action of the fucans is selective inhibition of thrombin activity by heparin cofactor II. The distinctive tetrasaccharide repeating units contribute to the anticoagulant action. Additionally, unlike the fucans from marine alga, although the sea cucumber fucans have great molecular weights and affluent sulfates, they do not induce platelet aggregation. Overall, our results may be helpful in understanding the structure-function relationships of the well-defined polysaccharides from invertebrate as new types of safer anticoagulants.

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Determination of the Molecular Mass of New l-Fucose-Containing Glycosaminoglycan and Its Distribution by High-Performance Gel-Permeation Chromatography with Laser Light-Scattering Detection

Cited by 17 publications

References 20 publications

Structural Characterization and Immunostimulatory Activity of a Homogeneous Polysaccharide from Sinonovacula constricta

Structural Characterization and Immunostimulatory Activity of a Homogeneous Polysaccharide from Sinonovacula constricta

Comparison of Physicochemical Characteristics and Anticoagulant Activities of Polysaccharides from Three Sea Cucumbers

Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber

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