Determination of the nucleotide composition of a deoxyribonucleic acid by high-performance liquid chromatography of its enzymatic hydrlysate: a review

Kaneko, Tadaaki; Katoh, Kiyoaki; Fujimoto, Masao; Kumagai, Masao; Támaoka, Jin; Katayama-Fujimura, Yoko

doi:10.1016/0167-7012(86)90034-5

Cited by 60 publications

(25 citation statements)

References 19 publications

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“…DNAs were extracted according to the method of Auling et al (1986). After enzymic hydrolysis, the GjC content was determined by HPLC analysis of the component nucleosides (Kaneko et al, 1986). The HPLC apparatus was equipped with a Waters pump (model 510), a model U6K injector, a column oven, a UV detector (Waters 484) and a reversedphase column (250i4 mm ; hypersil C18 ; 5 µm particles).…”

Section: Methodsmentioning

confidence: 99%

Thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry.

Busse¹,

Kämpfer²,

Moore³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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Section: Methodsmentioning

confidence: 99%

Thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry.

Busse¹,

Kämpfer²,

Moore³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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“…The guanine-plus-cytosine (G + C) content of DNA was determined by using a high-performance liquid chromatograph method (12,31). Heat-denatured DNA was digested into nucleosides by using nuclease P, (EC 3.1.30.1; Yamasa Shoyu Co., Ltd., Choshi, Japan) and bacterial alkaline phosphatase (EC 3.1.3.1; Sigma (Chemical Co., St. Louis, Mo.).…”

Section: Methodsmentioning

confidence: 99%

Nocardia seriolae sp. nov. Causing Nocardiosis of Cultured Fish

Kudo¹,

Hatai²,

Seino³

1988

International Journal of Systematic Bacteriology

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were studied to establish their taxonomic status. Well-developed fragmenting vegetative mycelium was observed. The chemotype was type IVA containing rneso-diaminopimelic acid, arabinose, and galactose. The predominant isoprenoid quinone was MK-8(H4), and mycolic acids were present. These morphological and chemical properties are characteristic of the genus Nocurdiu. The physiological and biochemical characteristics were most similar to those of Nocurdiu usteroides; however, these organisms were different in their decomposition of urea, growth temperature, utilization of nitrogen sources, survival at 50°C for 8 h, mycolic and fatty acid profiles, and deoxyribonucleic acid-deoxyribonucleic acid hybridization characteristics. Therefore, we propose a new species for these strains, Nocurdiu seriolae. The type strain is strain JCM 3360.A disease caused by actinomycetes in cultured fishes occurred first in rudderfishes (Seriola quinqueradiata and Seriola purpurascens) in Mie Prefecture, Japan, in 1967 (2, 13) and then spread to fish farms in the western district of Japan. This disease is characterized by the formation of abscesses in the epidermis and of tubercles in gills, kidneys, and spleens (13,15,17, 18). In 1968, the causative organisms were initially isolated, and their morphological, physiological, and biochemical characteristics were examined by Kariya et al. (13). Although these authors suggested that the strains were related to Nocardia asteroides, they proposed a new species, "Nocardia kampachi," for these organisms because of differences in some physiological and biochemical characteristics. However, morphology was the sole characteristic used for assignment of these strains to the genus Nocardia, and the description of " N . kampachi" lacked designation of a type strain. This name has never been validated, and original strains are not available anymore. Later, in 1973 and1974, Kusuda and Taki (17) and Kusuda et al. (18) isolated bacteria from infected yellowtails and identified them as " N . kampachi."We studied one of the strains of Kusuda (17) and four strains newly isolated from infected fishes (yellowtails [S. quinqueradiata] and a Japanese flounder [Paralichthys olivaceus]) to establish the taxonomic status of these strains. Based on morphological and chemical properties, we assigned the isolates to the genus Nocardia, and we propose a new species, Nocardia seriolae, for them on the basis of deoxyribonucleic acid (DNA)-DNA hybridization results and mycolic and fatty acid profiles in addition to physiological and biochemical characteristics. MATERIALS AND METHODSBacterial strains. In this study we used five strains isolated from cultured fishes from various fish farms in Japan and, for comparison, some strains of N . asteroides and related strains (Table 1).Morphology. The morphology of the isolates was studied by growing the strains on dilute yeast extract-malt extract agar (ISP medium no. 2 diluted to a 1/10 concentration) at 28°C for 5 days. A sample for electron microscopy was * Corresponding au...

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“…Purified DNA was dissolved in Nuclease P1 buffer (40 mM sodium acetate, 2 mM ZnSO % , pH 5n3). Determination of the GjC content was done by HPLC as described previously (Kaneko et al, 1986).…”

Section: Sample Collection Bacterial Strains and Cultural Conditionsmentioning

confidence: 99%

Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov.

Buczolits¹,

Denner²,

Vybiral³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

102

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Determination of the nucleotide composition of a deoxyribonucleic acid by high-performance liquid chromatography of its enzymatic hydrlysate: a review

Cited by 60 publications

References 19 publications

Thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry.

Thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry.

Nocardia seriolae sp. nov. Causing Nocardiosis of Cultured Fish

Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov.

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