Determination of the phenolic, antioxidant and antimicrobial potential of leaf extracts of Pereskia grandifolia Haw

Vicente, Nelma Ferreira de Paula; Martins, Heloísa Helena de Abreu; Campidelli, Marina Leopoldina Lamounier; Silva, Diogo Mendes da; Aazza, Smail; Souza, Elias Costa de; Bertolucci, Suzan Kelly Vilela; Píccoli, Roberta Hilsdorf

doi:10.33448/rsd-v9i10.8483

Cited by 3 publications

(4 citation statements)

References 39 publications

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“…Together, the results of this research contribute to the growing evidence supporting the therapeutic potential of medicinal plants in the management of liver diseases, providing valuable insights for the development of treatments and the advancement of hepatic investigations [40][41][42]. It is important to highlight that the observed therapeutic efects can be attributed to the presence of bioactive compounds in P. grandifolia, such as phenolic compounds, favonoids and saponins, for example [43][44][45]. However, further research is necessary to identify and characterize the specifc compounds present in the extract utilized in this study.…”

Section: Discussionmentioning

confidence: 78%

Bridging the Gap: Exploring the Preclinical Potential of Pereskia grandifolia in Metabolic‐Associated Fatty Liver Disease

Rodrigues Albuquerque,

Ratti da Silva,

de Abreu Braga

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Metabolic-associated fatty liver disease (MAFLD) is a complex condition characterized by steatosis and metabolic disturbances. Risk factors such as diabetes, cigarette smoking, and dyslipidaemia contribute to its development and progression. Effective and safe therapies for MAFLD are urgently needed. Pereskia grandifolia has shown potential as an alternative treatment, but its effectiveness against liver disease remains unexplored. This research aims to determine the hepatoprotective properties of P. grandifolia using a model of MAFLD. The study was carried out through various phases to assess the safety and efficacy of the ethanol-soluble fraction of P. grandifolia. Initially, an in vitro assay was performed to assess cell viability. This was followed by an acute toxicity test conducted in rats to determine the safety profile of the extract. Subsequently, the anti-inflammatory properties of P. grandifolia were examined in macrophages. For the MAFLD study, diabetic Wistar rats were made diabetic and exposed to a high fat diet and cigarette smoke, for 4 weeks. During the last 2 weeks, the rats were orally given either the vehicle (negative control group; C-), P. grandifolia (30, 100, and 300 mg/kg), or insulin in addition to simvastatin. A basal group of rats not exposed to these risk factors was also assessed. Blood samples were collected to measure cholesterol, triglycerides, glucose, ALT, and AST levels. Liver was assessed for lipid and oxidative markers, and liver histopathology was examined. P. grandifolia showed no signs of toxicity. It demonstrated anti-inflammatory effects by inhibiting phagocytosis and macrophage spreading. The MAFLD model induced liver abnormalities, including increased AST, ALT, disrupted lipid profile, oxidative stress, and significant hepatic damage. However, P. grandifolia effectively reversed these changes, highlighting its potential as a therapeutic agent. These findings emphasize the significance of P. grandifolia in mitigating hepatic consequences associated with various risk factors.

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Section: Discussionmentioning

confidence: 78%

Bridging the Gap: Exploring the Preclinical Potential of Pereskia grandifolia in Metabolic‐Associated Fatty Liver Disease

Rodrigues Albuquerque,

Ratti da Silva,

de Abreu Braga

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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“…In addition, the leaf mass of ora-pro-nobis has vitamins A, B and C, important for the immune process, eyes and skin (Vicente et al, 2020;Francelin et al, 2021). The absence of foliar toxicity makes it an important and useful food (Almeida-Filho and Cambraia, 1974), characterizing a complementary source in the diet of many Brazilians (Almeida et al, 2014;Takeiti et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

“…It is rich in soluble fiber, important for the digestive and intestinal process. It contains vitamins A, B and C, important for the immune process, eyes and skin, in addition to minerals, such as iron, calcium and phosphorus (Vicente et al, 2020;Francelin et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Ora-pro-nobis - chemical characterization and sourcing of crude extract through different extraction methods: a review

Carnevalli

Ramos

Mardigan

et al. 2022

RSD

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Ora-pro-nobis has its center of origin in tropical America. It is found in Brazil, from the Northeast to Rio Grande do Sul. The plant belongs to the Cactaceae family, Pereskia genus. Its leaves and fruits contain bioactive compounds that may have expectorant properties and can be used to alleviate inflammatory processes in traditional medicine. Despite its richness, the plant is still little used by the general population and the food and pharmaceutical industries. Some extraction methods are used to obtain bioactive compounds from crude plant extracts, such as maceration, supercritical fluid, and ultrasound-assisted extraction, which have stood out for meeting the principles of green chemistry. The chemical characterization of the extracts obtained through chromatographic analysis by GC-MS/MS and LC-MS/MS proves to be extremely effective, as it allows quantifying the various chemical species present in the extract, making it possible to evaluate and compare the results generated in each chosen extraction condition. The objective of this research is to conduct a review study about the Pereskia aculeata Miller plant. To this end, an exploratory study was carried out, by means of theoretical surveys through Science direct, Capes, Scielo and Pubmed databases. Extracting from plants using different methods is an important step for the industry, ensuring improvement in the quality of products, especially in the food and pharmaceutical sectors. In this sense, no comparative study was found in the literature employing different extraction methods for ora-pro-nobis in the sense of identifying the best method, one with better yield and greater amount of bioactive compounds. The search for different extraction strategies integrated with processes and products that can extract intelligently, minimizing waste, shows an innovative potential in future research.

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“…Additionally, cytotoxic effects on cancer and normal cell lines [8,9,49,67,68], anti-inflammatory activity [11], and cholinergic enzyme inhibitory capacity [36] have also been investigated. Aqueous and hydroalcoholic extracts of P. grandifolia leaves had their antibacterial activity tested against S. aureus and P. aeruginosa [69], and the authors found that extracts showed inhibitory activity against the tested bacteria. However, only the hydroalcoholic extract obtained by reflux was bactericidal at the tested concentrations.…”

Section: Extraction Of the Bioactives Of P Aculeata And P Grandifoliamentioning

confidence: 99%

A Critical Appraisal of the Most Recent Investigations on Ora-Pro-Nobis (Pereskia sp.): Economical, Botanical, Phytochemical, Nutritional, and Ethnopharmacological Aspects

Teixeira,

Oliveira,

Backes

et al. 2023

Plants

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Pereskia aculeata Miller and Pereskia grandfolia Haw, known as ‘ora-pro-nobis’, are unconventional vegetables belonging to the Cactaceae family, native to the Americas and common in the northeast and southeast regions of Brazil. This review attempts to present a balanced account of both the methods used for obtaining extracts from the diverse parts of the plants and the results that were obtained in terms of their applicability to foods and other products with biological activities. Attention will also be devoted to the properties of their bioactives and their applications to real food products. Methods for obtaining extracts from the diverse parts of the plants will be analyzed, as well as the chemical nature of the bioactives that were hitherto identified. Next, the applicability of ora-pro-nobis in either its integral form or in the form of extracts or other products (mucilages) to the production of food and dietary supplements will be analyzed. The species have been extensively investigated during the last few decades. But, the determination of chemical structures is frequently incomplete and there is a need for new studies on texture determination and color evaluation. Further studies exploring the fruit and flowers of P. aculeata are also required.

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Determination of the phenolic, antioxidant and antimicrobial potential of leaf extracts of Pereskia grandifolia Haw

Cited by 3 publications

References 39 publications

Bridging the Gap: Exploring the Preclinical Potential of Pereskia grandifolia in Metabolic‐Associated Fatty Liver Disease

Bridging the Gap: Exploring the Preclinical Potential of Pereskia grandifolia in Metabolic‐Associated Fatty Liver Disease

Ora-pro-nobis - chemical characterization and sourcing of crude extract through different extraction methods: a review

A Critical Appraisal of the Most Recent Investigations on Ora-Pro-Nobis (Pereskia sp.): Economical, Botanical, Phytochemical, Nutritional, and Ethnopharmacological Aspects

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