Determination of the phenolic content and antioxidant potential of crude extracts and isolated compounds from leaves of<i>Cordia multispicata</i>and<i>Tournefortia bicolor</i>

Silva, Thiago B. Correia Da; Souza, Vivian Karoline T.; Silva, Ana Paula F. Da; Lemos, Rosângela P. Lyra; Conserva, Lúcia M.

doi:10.3109/13880200903046146

Cited by 15 publications

(11 citation statements)

References 23 publications

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“…Lipid peroxidation is widely used as marker of stress-induced damage. In this study, MDA content, a by-product of lipid peroxidation (da Silva et al, 2010) was used as an indirect measurement. Our results indicate that MDA levels increased in root tissue when Cd 2+ , and especially Cu 2+ , were added to the irrigation solution, indicating that oxidative damage occurred.…”

Section: Discussionmentioning

confidence: 99%

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NaCl protects against Cd and Cu-induced toxicity in the halophyte Atriplex halimus

Bankaji¹,

Sleimi²,

Gómez-Cádenas

2017

Span. j. agric. res.

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The objective of the present work was to evaluate the extent of Cd-and Cu-induced oxidative stress and the antioxidant response triggered in the halophyte species Atriplex halimus after metallic trace elements exposure. Plants were treated for one month with Cd 2+ or Cu 2+ (400 µM) in the absence or presence of 200 mM NaCl in the irrigation solution. The interaction between salinity and heavy metal stress was analyzed in relation to plant growth, tissue ion contents (Na + , K + and Mg 2+ ), oxidative damage and antioxidative metabolism. Data indicate that shoot and root weight significantly decreased as a consequence of Cd 2+ -or Cu 2+ -induced stress. Metallic stress leads to unbalanced nutrient uptake by reducing the translocation of K + and Mg 2+ from the root to the shoot. The levels of malondialdehyde increased in root tissue when Cd, and especially Cu, were added to the irrigation solution, indicating that oxidative damage occurred. Results showed that NaCl gave a partial protection against Cd and Cu induced toxicity, although these contaminants had distinct influence on plant physiology. It can be concluded that salinity drastically modified heavy metal absorption and improved plant growth. Salinity also decreased oxidative damage, but differently in plants exposed to Cd or Cu stress.Additional key words: Atriplex halimus; oxidative damage; trace element toxicity; salinity Abbreviations used: APX (ascorbate peroxidase); CAT (catalase); DTNB (dinitrobenzoic acid); FW (fresh weight); GPX (guaiacol peroxidase); GSH (reduced glutathione); GSSG (oxidised glutathione); MDA (malondialdehyde); PC (phytochelatin); ROS (reactive oxygen species); SOD (superoxide dismutase); tGSH (total GSH) Authors' contributions: Cation determination assays: IB. Coordinated the obtention and culture of the plants and was responsible for conducting different experiments and the evaluation of plants: NS. Responsible of the study of the antioxidative system, evaluating the antioxidant enzymatic activities as well as MDA; he also was in charge of glutathione and phytochelatin content determination: AGC. Coordination of the works and preparing the manuscript: RMPC.Citation: Bankaji, I.; Sleimi, N.; Gómez-Cadenas, A.; Pérez-Clemente, R. M. (2016). NaCl protects against Cd and Cu-induced toxicity in the halophyte Atriplex halimus.

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Section: Discussionmentioning

confidence: 99%

“…The increase of lipid peroxidation products such as malondialdehyde (MDA) as a consequence of the oxidative stress has been considered as an indicator of free radical production and tissue damage. Actually, lipid peroxidation alters membrane properties such as fluidity and permeability and thereby its functions (da Silva et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

NaCl protects against Cd and Cu-induced toxicity in the halophyte Atriplex halimus

Bankaji¹,

Sleimi²,

Gómez-Cádenas

2017

Span. j. agric. res.

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“…A majority of the triterpenoid compounds were identified from leaves and other few from root, stem, seed, fruit and aerial parts. As per structural characteristics, they are classified into four classes: (1) oleanane type isolated from C. alliodora, C. cylindrostachya, C. piauhiensis, C. latifolia and C. multispicata ; (2) lupane type identified from C. obliqua and C. gilletii ; (3) ursane type reported from C . retusa, C. obliqua, C. rothii and C. multispicata ; and (4) dammarane type found in C. spinescens, C. multispicata and C. verbenacea .…”

Section: Phytochemistry Of the Genus Cordiamentioning

confidence: 99%

“…Up till now three diterpenes, that is trans‐phytol, cordioic acid and cordifolic acid, have been identified from C. multispicata and C. latifolia . Biological activity of these compounds has not been evaluated (Table 2.3).…”

Section: Phytochemistry Of the Genus Cordiamentioning

confidence: 99%

Traditional uses, phytochemistry and pharmacology of the medicinal species of the genus Cordia (Boraginaceae)

Oza

Kulkarni

2017

Journal of Pharmacy and Pharmacology

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Objectives Cordia (family Boraginaceae) is a genus of deciduous flowering trees or shrubs comprising more than 300 species distributed widely in the tropical regions. The aim of this review was to provide exhaustive scientific information on traditional uses, phytochemistry and pharmacological activities of the 36 important species with medicinal value from the genus Cordia, to divulge prospects for further research on its therapeutic potential. Key findings Leaves, fruit, bark and seed of a majority of the species were found to possess abundant ethnomedicinal value, but leaves were found to be used most frequently to treat many ailments such as respiratory disorders, stomach pain, wound, inflammation, myalgia, cough, dysentery and diarrhoea. The phytochemical investigation of 36 species resulted in isolation of 293 chemical constituents from various chemical classes. The crude extracts, fractions, essential oils and pure compounds isolated from various Cordia species were reported to have a varied range of pharmacological activities. Summary Many of the traditional uses of the genus Cordia were supported by the results obtained from pharmacological studies performed using various extracts or pure compounds. More attention should be given to the biological evaluation using pure phytochemicals and to identify the mechanism of actions and exploring this genus for new drug discovery.

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“…Taraxerol from other plant has already been reported to have plenty of pharmacological activities like antitumor, antiinflammatory, antiproliferative, and antioxidant etc. [13–16] These reports suggest that C. ternatea may also have potential therapeutic application over skin wound due to the presence of taraxerol. A better separation of the taraxerol marker in the extract was noted by the peak purity analysis.…”

Section: Discussionmentioning

confidence: 99%

Standardized Clitoria ternatea leaf extract as hyaluronidase, elastase and matrix-metalloproteinase-1 inhibitor

et al. 2012

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Aim:Plant Clitoria ternatea L. is claimed to possess a wide range of activities including antiinflammatory, local anesthetic and antidiabetic effect, etc. The aim of the present study was to evaluate the wound healing potential of standardized C. ternatea leaf extract in terms of different enzymatic models, which are mostly associated with skin wound.Materials and Methods:The methanol extract and fractions were screened for its hyaluronidase, elastase, and matrix metalloproteinase-1 (MMP-1) inhibitory activity compared with standard oleanolic acid. The activity was rationalized through reverse phase high performance liquid chromatography (RP-HPLC) standardization of the extract and fractions with respect to its isolated biomarker taraxerol (yield 5.27% w/w).Results:The extract showed significant (P < 0.001) hyaluronidase (IC50 18.08 ± 0.46 μg/ ml) and MMP-1 (P < 0.05) inhibition, but the elastase inhibition was insignificant (IC50 42.68 ± 0.46 μg/ml). Among the fractions, ethyl acetate fraction showed significant (P < 0.001) inhibition of hyaluronidase (IC50 28.01 ± 0.48 μg/ml) and MMP-1 (P < 0.01). The HPLC analysis revealed that the extract and the ethyl acetate fraction are enriched with taraxerol (5.32% w/w and 4.55% w/w, respectively).Conclusions:The experiment validated the traditional uses of C. ternatea and may be recommended for use in the treatment of different types of skin wounds, where taraxerol may be a responsible biomarker.

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Determination of the phenolic content and antioxidant potential of crude extracts and isolated compounds from leaves ofCordia multispicataandTournefortia bicolor

Cited by 15 publications

References 23 publications

NaCl protects against Cd and Cu-induced toxicity in the halophyte Atriplex halimus

NaCl protects against Cd and Cu-induced toxicity in the halophyte Atriplex halimus

Traditional uses, phytochemistry and pharmacology of the medicinal species of the genus Cordia (Boraginaceae)

Standardized Clitoria ternatea leaf extract as hyaluronidase, elastase and matrix-metalloproteinase-1 inhibitor

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