Determination of theophylline and its metabolites in biological samples by liquid chromatography–mass spectrometry

Kanazawa, Hideko; Atsumi, Ryoko; Matsushima, Yoshikazu; Kizu, Junko

doi:10.1016/s0021-9673(99)00891-2

Cited by 60 publications

(27 citation statements)

References 9 publications

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“…All these methods lack precision and accuracy and are mainly used for screening purposes. There are more quantitative methods available from the literature, which mainly use technology such as HPLC-UV (Delbeke and De Backer, 1996;Georga et al, 2001;Bispo et al, 2002), GC-MS (Houghton, 1982;Kress et al, 2002) and LC-MS (Kanazawa et al, 2000). The HPLC-UV methods have limited specificity and GC-MS methods are not practical and require extensive sample preparation and derivatization steps.…”

Section: Introductionmentioning

confidence: 99%

Development of an electrospray ionization mass spectrometric method for the quantification of theophylline in horse serum

Beaudry

Lavoie

Vachon

2005

Biomed. Chromatogr.

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A rapid and selective method has been developed for the determination of theophylline in horse serum by LC-ESI/MS/MS. The analytical method includes a protein precipitation extraction for sample preparation, liquid chromatography separation technique and ionspray tandem mass spectrometry. The drug was extracted from serum using a protein precipitation with acetonitrile and the supernatants were analyzed using an LC-ESI/MS/MS instrument. The chromatography was performed using a 50 x 2.1 mm C(8) analytical column and an isocratic mobile phase composes of 60:40 acetonitrile-0.5% formic acid in water with a flow rate fixed at 350 microL/min. A linear (weighted 1/concentration) relationship was used to perform the calibration over an analytical range of 0.1-20 ppm. The intra-batch precision and accuracy at LLOQ, medium and high concentration were 11.7, 6.9 and 5.4% and 95.8, 107.8 and 95.8%, respectively, and the inter-batch precision and accuracy at LLOQ, medium and high concentration were 10.4, 7.9 and 7.3% and 97.3, 105.2 and 95.9%, respectively. This LC-ESI/MS/MS method for the determination of theophylline in horse serum has been proved to within generally accepted criteria used for bioanalytical assay and was used successfully during clinical investigation.

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Section: Introductionmentioning

confidence: 99%

Development of an electrospray ionization mass spectrometric method for the quantification of theophylline in horse serum

Beaudry

Lavoie

Vachon

2005

Biomed. Chromatogr.

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“…LC/MS methods of analysis for salicylic acid, 6) acetaminophen, [6][7][8] theophylline, 9,10) barbiturates, 11) and bromvalerylurea [12][13][14] have been reported, but our examination of the literature has not revealed any method of simultaneous analysis for all of these from biological samples.…”

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confidence: 83%

Method for Screening and Quantitative Determination of Serum Levels of Salicylic Acid, Acetaminophen, Theophylline, Phenobarbital, Bromvalerylurea, Pentobarbital, and Amobarbital Using Liquid Chromatography/Electrospray Mass Spectrometry

Hori

Fujisawa

Shimada

et al. 2006

Biological & Pharmaceutical Bulletin

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“…Whereas for levosalbutamol sulfate a few spectrophotometric Thulasama et al, 2009) and HPLC (Ghemud et al, 2012;Halabi et al, 2004; methods are reported. The reported methods for theophylline includes spectrophotometric (Abuirjeie et al, 1992;Bermejo et al, 1985;Carter et al, 1985;Fellenberg et al, 1979;Hohnadel et al, 1978;Jatlow, 1975;Kumar et al, 2011;Plavsic, 1978;Vasiliades et al, 1976), HPLC (Abuirjeie et al, 1992;Boberic-Borojevic et al, 1999;Greenberg et al, 1979;Lauff, 1987;Moncrieff, 1991;Papadoyannis et al, 1993;Shidhaye et al, 2009), GC (Chrzanowski et al, 1974;Schwertner, 1979;Shah et al, 1974) and LC-MS (Abdel-Hamid et al, 2003;Babu et al, 2011;Kanazawa et al, 2000;Song et al, 2004). The literature survey suggests there is lack of a stability indicating RP-HPLC method for simultaneous determination of levosalbutamol sulfate and theophylline in combined syrup dosage forms.…”

Section: Introductionmentioning

confidence: 99%

Stability-indicating RP-HPLC method for simultaneous estimation of levosalbutamol sulfate and theophylline in combined dosage form

Panda

Kumar

Mohanta

2013

Braz. J. Pharm. Sci.

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A novel, simple, accurate and precise RP-HPLC method for simultaneous determination of levosalbutamol sulfate and theophylline has been developed and validated. Separation was achieved on a Phenomenex; C 18 column (250 mm × 4.6 mm i.d., 5 μm) using methanol: 10 mM TBAHS(tetrabutyl ammonium hydrogen sulfate) (50:50, v/v) as mobile phase at flow rate of 1.0 mL.min -1 . The UV detection wavelength was 274 nm. The linearity is obeyed over a concentration range of 0.5-150 μg.mL -1 with correlation coefficient of 0.999 for both the drugs. The proposed method was validated by determining accuracy, precision, stability and system suitability parameters. The method was found to be robust. Specificity of the method was determined by subjecting the drugs to various stress conditions like acid, alkali, oxidation, thermal and photolytic degradation. The method was used successfully for the simultaneous determination of levosalbutamol sulfate and theophylline in syrup dosage form.Uniterms: Levosalbutamol/determination. Theophylline/determination. Stability-indicating. RP-High performance liquid chromatography/qualitative analysis Syrup/qualitative analysis.Desenvolveu-se e validou-se método de RP-HPLC novo, simples, exato e preciso de determinação simultânea do sulfato de levossalbutamol e teofilina.. A separação foi efetuada em uma coluna Phenomenex; C18 (250 mm x 4,6 mm d.i., 5 μm) utilizando metanol: TBAHS (hidrogenossulfato de tetrabutilamônio) 10 mM (50:50, v/v) como fase móvel, com fluxo de 1,0 mL.min -1 . O comprimento de onda de detecção no UV foi 274 nm. Observou-se linearidade na faixa de concentração de 0,5-150 μg mL -1 , com coeficiente de correlação de 0,999 para ambos os fármacos. O método proposto foi validado determinando-se exatidão, precisão, estabilidade e parâmetros de adequação do sistema. O método mostrou-se robusto. A especificidade do método foi determinada submetendo os fármacos a várias condições de estresse, como ácido, álcali, oxidação, degradação térmica e fotolítica. O método foi usado com sucesso para a determinação simultânea do sulfato de levossalbutamol e teofilina na forma de xarope.

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Determination of theophylline and its metabolites in biological samples by liquid chromatography–mass spectrometry

Cited by 60 publications

References 9 publications

Development of an electrospray ionization mass spectrometric method for the quantification of theophylline in horse serum

Development of an electrospray ionization mass spectrometric method for the quantification of theophylline in horse serum

Method for Screening and Quantitative Determination of Serum Levels of Salicylic Acid, Acetaminophen, Theophylline, Phenobarbital, Bromvalerylurea, Pentobarbital, and Amobarbital Using Liquid Chromatography/Electrospray Mass Spectrometry

Stability-indicating RP-HPLC method for simultaneous estimation of levosalbutamol sulfate and theophylline in combined dosage form

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