Method for Screening and Quantitative Determination of Serum Levels of Salicylic Acid, Acetaminophen, Theophylline, Phenobarbital, Bromvalerylurea, Pentobarbital, and Amobarbital Using Liquid Chromatography/Electrospray Mass Spectrometry

Hori, Yasushi; Fujisawa, Manami; Shimada, Kenji; Hirose, Yasuo; Yoshioka, Toshiharu

doi:10.1248/bpb.29.7

Cited by 48 publications

(28 citation statements)

References 14 publications

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“…The detection of APAP in an actual hair specimen obtained from an acute poisoning case revealed that APAP is incorporated into human hair. Recently, liquid chromatography (LC)-MS [12] and LC-MS-MS [13] have gained popularity in drug analyses. However, the GC-MS technique has various advantages over the LC-MS (-MS), because GC-MS instruments are generally much cheaper and are now very common in forensic laboratories.…”

Section: Discussionmentioning

confidence: 99%

GC-MS assay for acetaminophen in human hair segments

et al. 2008

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Section: Discussionmentioning

confidence: 99%

GC-MS assay for acetaminophen in human hair segments

et al. 2008

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“…20,29 Moreover, clean extracts were obtained by using this sorbent when preliminary experiments have been performed to test its suitability. Oasis HLB is a polymeric sorbent with a polar group, with a hydrophilic-lipophilic-balance and reversed-phase interactions.…”

Section: Drug Analysis By In-line Spe-ce-uvmentioning

confidence: 99%

“…This selection was based on the existing literature regarding the off-line SPE process involving barbiturates and Oasis HLB. 20,[29][30][31] The elution plug for the different mixtures tested was introduced into the capillary at 50 mbar for 30 s and was then pushed through the SPE sorbent by introducing the BGE solution at 50 mbar for 220 s. The results demonstrated that the differences in the organic solvent and mixtures tested, in terms of peak area, were not significant. Thus, MeOH was chosen as the elution solvent for further experiments.…”

Section: Study Of the Elution Stepmentioning

confidence: 99%

In-line Solid-phase Extraction–Capillary Zone Electrophoresis for the Determination of Barbiturate Drugs in Human Urine

et al. 2014

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The abuse of barbiturate drugs is widespread, and the development of methods for their efficient separation and quantification is needed. Three barbiturate drugs were preconcentrated and determined by in-line solid-phase extraction (SPE) capillary electrophoresis (CE) in urine samples. Different parameters affecting preconcentration were evaluated, such as the sample pH, the volume of the elution plug and the sample injection time. This strategy enhanced the detection sensitivity in the range of 170-to 1840-fold, compared with normal hydrodynamic injection. The method provides limits of detection (LODs) for standard samples in the range of 0.5 to 5 ng/mL with good repeatability and reproducibility values. The LODs obtained for urine samples were in the range of 5 to 60 ng/mL. The validation with human urine samples spiked with the studied compounds demonstrated the applicability of the optimized method. This method provides a reproducible and sensitive analysis of urine samples in the determination of barbiturates drugs.

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“…In recent years, several LC-MS and LC-MS/MS methods have been reported for the analysis of acetaminophen and its major urinary metabolites, either alone or in combination with other drugs in biological samples (e.g., Refs. [5][6][7][8][9][10][11][12][13]). In contrast, GC-MS methods for acetaminophen are rare.…”

Section: Introductionmentioning

confidence: 99%