Determination of thiamine (vitamin B,) and riboflavin (vitamin B2) in meat and liver by high-performance liquid chromatography

Barna, E.; Dworschák, E.

doi:10.1016/0021-9673(94)80126-6

Cited by 56 publications

(34 citation statements)

References 5 publications

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“…Due to the extensive phosphorylated and protein-bound vitamins in natural foods, quantitative analysis of the entire array of B-group vitamins at endogenous levels requires vigorous sample extraction to release the free vitamins using strong acids and enzyme hydrolysis. [19][20][21][22][23][24][25][26][27][28][29][30] However, these tedious, complex and harsh methods are not necessary to extract the vitamins from vitamin-supplemented food products.…”

Section: Resultsmentioning

confidence: 99%

“…[19][20][21][22]24,25,27,30 Interference in the chromatograms and poor recovery indicate degradation of some vitamins due to the harsh acid. Organic solvents can also be used to precipitate proteins and are milder toward the samples.…”

Section: Resultsmentioning

confidence: 99%

“…Extraction techniques based on different acid treatments and a These authors contributed equally to this work. enzymatic digestion such as hydrochloric acid, sulfuric acid, perchloric acid or amylase and taka-diastase [19][20][21][22][23][24][25][26][27][28][29][30] have been developed, but the acids or enzymes require a long incubation (normally over 10-h) and high heat. Thus, these processes may decrease the recovery of unstable vitamins, such as folic acid.…”

Section: -17mentioning

confidence: 99%

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Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Suh¹,

Yang²,

Lee³

et al. 2011

Bulletin of the Korean Chemical Society

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A simple HPLC-DAD method was developed and validated to determine B group vitamin content (thiamine, riboflavin, nicotinamide, pantothenic acid, pyridoxine and folic acid) in supplemented food samples, i.e., infant formula, cereal, low-calorie food, a multi-vitamin pill and a vitamin drink. In this study, the most significant advantages were simultaneous determination of the six B group vitamins in various food matrices and a small number of sample treatment steps that required only an organic solvent, acetonitrile. Moreover, this method prevents reduction of column durability, because the mobile phase does not contain ion-pairing reagents. Analytes were separated on a Develosil RPAQUEOUS C 30 (4.6 mm × 250 mm, 5 µm particle size) column with a gradient elution of acetonitrile and 20 mM phosphate buffer (pH 3.0) at a flow rate between 0.8 and 1.0 mL/min. Detection was performed at 275 nm, except for that of pantothenic acid (205 nm). The calibration curves for all six vitamins showed good linearity with correlation coefficients (r 2 ) higher than 0.995. The developed method was validated with respect to linearity, intra-and inter-day accuracy and precision, limit of quantification (LOQ), recovery and stability. The method showed good precision and accuracy, with intra-and inter-assay coefficients of variation less than 15% at all concentrations. The recovery was carried out according to the standard addition procedure, with yields ranging from 89.8 to 104.4%. This method was successfully applied to the determination of vitamin B groups in supplemented food products.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: -17mentioning

confidence: 99%

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Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Suh¹,

Yang²,

Lee³

et al. 2011

Bulletin of the Korean Chemical Society

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“…Amin and Reusch (1987) developed a simple, quick, specific and sensitive HPLC method for the determination of four of the B vitamins in a single run in the presence of each other, enabling vitamins to be investigated in the nanogram range as required for pharmacokinetics and bioavailability studies. Barna and Dworschak (1994) assayed vitamins B1 and B2 in meat and the liver by HPLC using phosphate buffer (pH 3.0) -acetonitrile (84:16, v/v) containing 5 mM sodium heptasulphonate. A different mobile phase mix of acetonitrile (85:15, v/v) was used by them for the B1 and B2 determination in liver in the UV-vis detector at 254 nm.…”

Section: Simultaneous Determination Of Water-soluble Vitaminsmentioning

confidence: 99%

Simultaneous detection of water-soluble vitamins using the High Performance Liquid Chromatography (HPLC) - a review

Abano

Dadzie

2014

Croat. J. Food Sci. Technol.

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SummaryThe water-soluble vitamins (WSV): ascorbic acid (vitamin C), thiamine (B1), riboflavin (B2), niacin (B3), panthothenic acid (B5), pyridoxine, and pyridoxal (B6), folic acid (B9), biotin(B8) , and B12 are very essential in the diet of humankind. As a result of ever increasing pressures from both consumers and legal enforcers, to specify accurately nutritive compositions of WSV that are present in food materials, many researchers have attempted to fill this niche through the provision of highly sensitive and rapid high performance liquid chromatography (HPLC) procedures. In view of the health benefits of WSV, a replete of HPLC methods have been developed for simultaneous determination of their contents in nature and fortified food samples, nutritional supplements, as well as blood plasmas. The rate of losses of these vitamins during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive HPLC procedure for their simultaneous separations and assays. This review critically assesses the different HPLC procedures developed by researchers and available in the open literature for simultaneous determination of water-soluble vitamins (WSV) in dried tropical fruits materials. The study revealed that not a single chromatographic run developed by researchers can simultaneously elute all the WSV at a time. However, the HPLC procedures that are capable of determining all the WSV were coupled with electrospray ionization mass spectroscopy (ESI-MS), thus making the set-up expensive.

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“…6 The other used techniques are spectrophotometry, 7-9 chemiluminescence, 10 capillary electrophoresis, [11][12][13] and the most frequently used HPLC. [14][15][16][17] These methods have demonstrated good sensitivity and selectivity, but their implementation requires specialised expensive equipment and the procedures may be rather complicated and time-consuming. Contrary, electrochemical methods have been of great interest because they are rapid, reliable, more economic and suitable for in situ analysis.…”

Section: Introductionmentioning

confidence: 99%

Simple, Rapid and Selective Chronopotentiometric Method for the Determination of Riboflavin in Pharmaceutical Preparations Using a Glassy Carbon Electrode

Brezo

Stojanović

Suturović

et al. 2015

ACSi

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A novel, simple, sensitive and reliable electrochemical method for the riboflavin determination using chronopotentiometry with glassy carbon electrode was developed. The most important instrumental parameters of chronopotentiometry including type and concentration of supporting electrolyte, initial potential and current range were examined and optimised in respect to riboflavin analytical signal. Riboflavin provided well defined reduction signal at -0.12 V vs. Ag/AgCl (3.5 mol/L KCl) electrode in 0.025 mol/L HCl. Under optimal conditions, linear response of riboflavin was observed in the concentration range of 0.2-70 mg/L with achieved limit of detection of 0.076 mg/L and limit of quantitation of 0.23 mg/L of riboflavin. Common vitamins and excipients did not interfere with the determination. The proposed method was successfully applied for determination of riboflavin in commercially available pharmaceutical preparations. The obtained results were in statistical agreement to the contents declared by manufacturer and to those obtained by HPLC used as a comparative method.

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Determination of thiamine (vitamin B,) and riboflavin (vitamin B2) in meat and liver by high-performance liquid chromatography

Cited by 56 publications

References 5 publications

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Simultaneous detection of water-soluble vitamins using the High Performance Liquid Chromatography (HPLC) - a review

Simple, Rapid and Selective Chronopotentiometric Method for the Determination of Riboflavin in Pharmaceutical Preparations Using a Glassy Carbon Electrode

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