Simultaneous detection of water-soluble vitamins using the High Performance Liquid Chromatography (HPLC) - a review

Abano, Ernest Ekow; Dadzie, Rosemond Godbless

doi:10.17508/cjfst.2014.6.2.08

Cited by 23 publications

(10 citation statements)

References 33 publications

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“…Vitamins and Methionine analysis were analysed using the liquid chromatographic method (Abano et al, (2014) and Cortés-Herrera et al, (2019). Water samples for vitamin and methionine analysis were filtered through 0.22µm polypropylene Calyx capsule filters and collected in high-density polyethylene (HDPE) dark bottles and stored frozen until analysis.…”

Section: Vitamins and Methionine Analysismentioning

confidence: 99%

Nutrients: trace metals, micronutrients, oestrogen and B-vitamin content of Osun River: A River that runs southwestern Nigeria into the Atlantic Gulf of Guinea

Afolabi-Balogun

Oni-Babalola

Adeleke

et al. 2020

Preprint

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Osun-Osogbo Grove has a long history of healing and therapeutic claims by adherent believers, in spite of advancement in medicine. Scientists made attempts at investigating the biodiversity of the Grove, till date, there has not been convergence point between science and these indigenous beliefs. This study identified the presence of therapeutic agents in the water of Osun-Osogbo River, paying attention to at least six parameters; vitamin, phosphate, nitrate, amino acid, hormone and trace metal. Water samples were taken from two different sites during pre, during and post raining sessions (April 2017 - September 2019) were analysed using High Performance Liquid Chromatography (HPLC), Gas Chromatography Mass Spectroscopy (GC-MS) and Atomic Absorption Spectrometer (AAS). Trace metal analysis revealed an average of 0.009-0.079 mg/Kg Zinc from site one and lower in site two. The mean value of manganese at both sites was virtually the same at 0.018-0.313 mg/kg, aluminum content was 0.045-0.179mg/Kg at site one, 0.050-0.192mg/kg at site two, cobalt was 0.024 mg/kg at site one, 0.026 mg/kg at site two while nickel was 0.006 mg/kg and 0.004 mg/kg for site one and two respectively. HPLC analysis shows mean Methionine content at both sites is higher than the FDA (56.6 ug/mL); site one had 74.41 ug/mL while site two had 57.11 ug/mL The mean values of two water-soluble vitamins; Thiamine (B1) was 3.758 mg/Kg and 2.355 mg/Kg while Pyridoxine (B6) was 0.108 mg/Kg and 0.072 mg/Kg at site one and two. GCMS analysis of steroidal content revealed values below lowest observed effect level (LOEL), testosterone (4.8 ng/L) and estrogen (2.4 ng/L) were still elevated while ethinylestradiol and estriol were ≥1.5 ng/L. Summarily, site one the major part for spiritual activities showed higher essential nutrient contents than site two which support the enrichment and potential therapeutic properties of the Osun river water. However, further scientific research is required to ensure that these therapeutic potentials supersede the toxicological effect.

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Section: Vitamins and Methionine Analysismentioning

confidence: 99%

Nutrients: trace metals, micronutrients, oestrogen and B-vitamin content of Osun River: A River that runs southwestern Nigeria into the Atlantic Gulf of Guinea

Afolabi-Balogun

Oni-Babalola

Adeleke

et al. 2020

Preprint

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“…The use of this column removes the need for an ion pair reagent in the mobile phase [385]. Finally, we encourage the reader toward a recent and ample review regarding fat- and hydrosoluble vitamins, respectively [386,387].…”

Section: The Common Ground Among Measurements Performed In Food Anmentioning

confidence: 99%

Liquid Chromatography Analysis of Common Nutritional Components, in Feed and Food

Cortés-Herrera

Artavia

Leiva

et al. 2018

Foods

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Food and feed laboratories share several similarities when facing the implementation of liquid-chromatographic analysis. Using the experience acquired over the years, through application chemistry in food and feed research, selected analytes of relevance for both areas were discussed. This review focused on the common obstacles and peculiarities that each analyte offers (during the sample treatment or the chromatographic separation) throughout the implementation of said methods. A brief description of the techniques which we considered to be more pertinent, commonly used to assay such analytes is provided, including approaches using commonly available detectors (especially in starter labs) as well as mass detection. This manuscript consists of three sections: feed analysis (as the start of the food chain); food destined for human consumption determinations (the end of the food chain); and finally, assays shared by either matrices or laboratories. Analytes discussed consist of both those considered undesirable substances, contaminants, additives, and those related to nutritional quality. Our review is comprised of the examination of polyphenols, capsaicinoids, theobromine and caffeine, cholesterol, mycotoxins, antibiotics, amino acids, triphenylmethane dyes, nitrates/nitrites, ethanol soluble carbohydrates/sugars, organic acids, carotenoids, hydro and liposoluble vitamins. All analytes are currently assayed in our laboratories.

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“…The concern of B vitamins for the welfare of human health has been increased over the past decades. Their increasing awareness has driven the today's global health industry and pharmaceuticals market to produce a lot of vitamin B supplements, and fortified vitamin B enriched nutrients with particular concentration on their accurate assays and analytical methods 3,8 . The current knowledge for the analytical assays of B vitamins is based on spectroscopic, chromatographic, chemical, or microbiological techniques with various extraction procedures 9 .…”

Section: Introductionmentioning

confidence: 99%

Estimation of Water-Soluble Vitamin B-Complex in Selected Leafy and Non-Leafy Vegetables by HPLC Method

et al. 2019

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A high performance liquid chromatographic method (HPLC) equipped with photodiode array detector (PDA) has been used to determine the water-soluble vitamin B complex ( B1, B2, B3, and B6) in eleven selected vegetables of Bangladesh. The results showed varied levels of vitamin B-complexes. Precisely, Thankuni leaves showed the highest contents of vitamin B1; 0.19 mg/100 g, and vitamin B2; 0.25 mg/100g. Higher content of vitamin B3 (0.59 mg/100 g) were quantified in Coriander leaves, but absent in Jute leaves. On the other hand, the maximum quantified amount of B6 (0.73 mg/100 g) was detected in Carrot. In contrast, there was no detectable vitamin B6 in Jute, and Mint leaves and Cabbage. The findings of the current study may supplement the current Food Composition Table for Bangladesh (FCTB) by providing nutritional information of vitamin B complex in leafy and non-leafy vegetables which can also be used for calculating the habitual dietary intake and/or nutritional survey purposes.

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Simultaneous detection of water-soluble vitamins using the High Performance Liquid Chromatography (HPLC) - a review

Cited by 23 publications

References 33 publications

Nutrients: trace metals, micronutrients, oestrogen and B-vitamin content of Osun River: A River that runs southwestern Nigeria into the Atlantic Gulf of Guinea

Nutrients: trace metals, micronutrients, oestrogen and B-vitamin content of Osun River: A River that runs southwestern Nigeria into the Atlantic Gulf of Guinea

Liquid Chromatography Analysis of Common Nutritional Components, in Feed and Food

Estimation of Water-Soluble Vitamin B-Complex in Selected Leafy and Non-Leafy Vegetables by HPLC Method

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