Determination of total antibiotic resistance in waterborne bacteria in rivers and streams in Northern Ireland: Can antibiotic-resistant bacteria be an indicator of ecological change?

Moore, John E.; Rao, J. R.; Moore, Peter J.A.; Millar, B. Cherie; Goldsmith, Colin E.; Loughrey, Anne; Rooney, Paul J.

doi:10.1007/s10452-009-9294-z

Cited by 32 publications

(19 citation statements)

References 14 publications

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“…For example, Moore et al's analysis of 12 bacterial isolates collected from rivers and lakes in Northern Ireland showed that 17%, 75%, and 75% of isolates were resistant to GEN, TET, and ERY, respectively (33). In our isolates, the respective resistances were a strikingly similar 17%, 79%, and 83%.…”

Section: Discussionsupporting

confidence: 53%

Association of Antibiotic Resistance in Agricultural Escherichia coli Isolates with Attachment to Quartz

Liu

Soupir

Zwonitzer

et al. 2011

Appl Environ Microbiol

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Surface water can be contaminated by bacteria from various sources, including manure from agricultural facilities. Attachment of these bacteria to soil and organic particles contributes to their transport through the environment, though the mechanism of attachment is unknown. As bacterial attachment to human tissues is known to be correlated with antibiotic resistance, we have investigated here the relationship between bacterial attachment to environmental particles and antibiotic resistance in agricultural isolates. We evaluated 203 Escherichia coli isolates collected from swine facilities for attachment to quartz, resistance to 13 antibiotics, and the presence of genes encoding 13 attachment factors. The genes encoding type I, EcpA, P pili, and Ag43 were detected, though none was significantly related to attachment. Quartz attachment was positively and significantly (P < 0.0038) related to combined resistance to amoxicillin/streptomycin/tetracycline/sulfamethazine/ tylosin/chlortetracycline and negatively and significantly (P < 0.0038) related to combined resistance to nalidixic acid/kanamycin/neomycin. These results provide clear evidence for a link between antibiotic resistance and attachment to quartz in agricultural isolates. We propose that this may be due to encoding by the responsible genes on a mobile genetic element. Further exploration of the relationship between antibiotic resistance and attachment to environmental particles will improve the understanding and modeling of environmental transport processes, with the goal of preventing human exposure to antibiotic-resistant or virulent microorganisms.

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Section: Discussionsupporting

confidence: 53%

Association of Antibiotic Resistance in Agricultural Escherichia coli Isolates with Attachment to Quartz

Liu

Soupir

Zwonitzer

et al. 2011

Appl Environ Microbiol

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“…Such bacteria have natural intrinsic resistance, as well as having the ability to acquire determinants from agricultural run-off and human wastewater discharge, which may contain antibiotic resistance organisms, as well as sublethal concentrations of metabolically active antibiotic. The tracking of such organisms to their source may help determine the source of fecal pollution in aquatic ecosystems (Moore et al, 2010). In our study, 74.4% lagoon, 84.6% sea and 77.6% stream isolates were resistant to ampicillin.…”

Section: Discussionmentioning

confidence: 62%

“…Likewise, the basis for the employment of this method was to determine the presence of the most resistant organisms from potentially mixed bacterial populations originating from clinical specimens, so that the most resistant phenotypes could be treated appropriately. Several publications reported that the potential for environmental waterborne organisms to be promiscuous and exchange their resistance genetic determinants, with each other and more importantly, with transient pathogens entering the water system, is a cause for concern (Kümmerer, 2004(Kümmerer, , 2009a(Kümmerer, , 2009b(Kümmerer, , 2009c; Moore et al, 2010). In addition, investigations about the negative effects of these bacterial groups on coastal ecosystems that reach the sea directly should be planned.…”

Section: Discussionmentioning

confidence: 99%

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Sivri¹,

Sandallı²,

Özgümüş³

et al. 2012

Turk. J. Fish. Aquat. Sci.

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The aim of this study is to find out the density of the fecal bacteria and to analyze resistance to antimicrobials of Gram negative bacilli isolated from the Kucukcekmece Lagoon, Istanbul. Samples were taken monthly from June 2006 to June 2008 and a total of 232 Gram negative bacilli were isolated. Chloramphenicol, tetracycline, nalidixic acid, ampicillin, imipenem, ceftazidime, amikacin, streptomycin and amoxicillin + clavulanic acid were used in antimicrobial susceptibility tests. Susceptibility to trimethoprim + sulfamethoxazole was also examined in only integron-bearing organisms. The antibiotic resistance tests resulted in bacteria being the most resistant against ampicillin (76.29%) and the most sensitive against amikacin (93.56%). Of 232 isolates, 20 (8.6%) coliforms harbored class 1 and/or class 2 integrons. DNA sequencing showed that variable regions of the integrons harbored various gene cassettes; dfrA12, dfrA15, dfrA17, aadA1, aadA2, aadA5, bla OXA-30 and sat2. Integrons were found in bacteria from all sampling areas except 12 and D3.In this study, the determination of bacterial identification of the species of Gram negative bacilli and their Antibiotic Resistance Profiles in the Kucukcekmece Lagoon for the first time was investigated. A finding indicates that there is a heavy fecal pollution in this lagoon environment, which might probably be resulted from the intensive anthropogenic facilities. The risk to public health could be the transfer of antibiotic resistance determinants from the bacterial isolates to normal microbiota bacteria of humans unless the effective precautions such as water treatment plants are taken. tetrasiklin, nalidiksik asit, ampisilin, imipenem, seftazidim, amikasin, streptomisin ve amoksisilin+klavulanik asit kullanılmıştır. Trimetoprim+sulfametoksazola yatkınlık da sadece integron taşıyan organizmalar için incelenmiştir. Antibiyotik direnç sonuçlarına göre bakterilerin en dirençli olduğu antibiyotik ampisilin (%76,29) ve en hassas olduğu antibiyotik ise amikasin (%6,44) olarak bulunmuştur. 232 izolatın 20 si (%8,6) sınıf 1 ve / veya sınıf 2 integron barındırmıştır. DNA sekanslama sonucunda, integronlara ait değişken bölgelerin barındırdığı gen kasetlerinin, DfrA12, dfrA15, dfrA17, aadA1, aadA2, aadA5, blaOXA-30 ve sat2 olduğu tespit edilmiştir. Bakterilere ait bu integronlar 12 ve D3 nolu istasyonlar dışındaki tüm örnekleme bölgelerinde bulunmuştur.Bu çalışmada, Küçükçekmece Lagünü'nde Gram negatif bakteri türlerinin identifikasyonu ve antibiyotik direnç profili ile ilgili çalışma ilk kez yapılmıştır. Muhtemelen yoğun antropojenik girdilerin sonucu olarak Lagün'de ağır bir fekal kirlilik olduğu görülmüştür. Atık su arıtma tesisleri gibi etkili önlemler alınmazsa, insanların normal mikrobiotasında yer alan bakteri izolatlarına, antibiyotik direnç genlerinin aktarımı ile halk sağlığı riski oluşması olasıdır.Anahtar Kelimeler: Gram negatif basiller, antibiyotik direnci, integron, Küçükçekmece Lagünü, İstanbul, Türkiye.

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“…Moore et al (2010), researching antimicrobial resistance in bacteria in the waters of rivers and streams in Northern Ireland, observed Cli, Van, Pen and Tcy resistant profiles. According to these authors, the resistance profile expressed by environmental strains allows the identification of the type of polluting source that the ecosystem has been receiving.…”

Section: Discussionmentioning

confidence: 99%

Multiple antibiotic-resistance of Enterococcus isolated from coastal water near an outfall in Brazil

Edirsana¹,

Renata²,

Alberto³

et al. 2014

Afr. J. Microbiol. Res.

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Marine outfalls contribute to the environmental protection of coastal zones. However, these structures may serve as vehicles for microbiological contamination. This study aimed to investigate the occurrence of antimicrobial-resistant bacteria in water samples collected from 67 stations located in nearby areas of the ocean outfall in Fortaleza, Brazil. 81 Enterococcus strains were isolated, identified and distributed in the following groups of species: Enterococcus faecalis (n = 37; 45.7%), Enterococcus faecium (n = 30; 37%), Enterococcus mundtii (n = 9; 11.1%), Enterococcus raffinosus (n = 2; 2.5%), Enterococcus dispar (n = 2; 2.5%) and Enterococcus durans (n = 1; 1.2%). Antimicrobial resistance was observed in 47 (58%) of the strains, and the most predominant profile was the concurrent resistance to ampicillin, clindamycin, penicillin and vancomycin. In 31 strains were detected phenotypically, plasmid resistance factors. The data reported in this study should serve as an alert to public health authorities, since they suggest that the area near the submarine outfall in Fortaleza may contribute to antimicrobialresistant enterococci spread.

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Determination of total antibiotic resistance in waterborne bacteria in rivers and streams in Northern Ireland: Can antibiotic-resistant bacteria be an indicator of ecological change?

Cited by 32 publications

References 14 publications

Association of Antibiotic Resistance in Agricultural Escherichia coli Isolates with Attachment to Quartz

Association of Antibiotic Resistance in Agricultural Escherichia coli Isolates with Attachment to Quartz

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Multiple antibiotic-resistance of Enterococcus isolated from coastal water near an outfall in Brazil

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