Determination of Total Phenolic Content, Analysis of Bioactive Compound Components, and Antioxidant Activity of Ethyl Acetate Seri (Muntingia calabura L.) Leaves from North Sumatera Province, Indonesia

Abstract

BACKGROUND: Seri (Muntingia calabura L.) leaves are a plant that is often found and have not been used in various treatments even though it is reported to have various groups of bioactive compounds such as phenolic, flavonoids, tannins, saponins, steroids, and triterpenoids. AIM: This study aimed to determine the total phenolic content, antioxidant activity and identify the content of potential bioactive compounds contained in the ethyl acetate fraction from M. calabura leaves. METHODS: M. calabura L. leaves fraction was carried out by maceration method using ethanol followed by partition starting with n-hexane, chloroform, and finally ethyl acetate as solvent. The ethyl acetate fraction was continued for phytochemical screening for the content of bioactive compounds using standard reagents, determination of total phenol content by colorimetric method, determination of antioxidant activity using the DPPH method, and analysis of bioactive compounds using gas chromatography–mass spectroscopy. RESULTS: The results showed that the ethyl acetate fraction of M. calabura leaves was positive for phenolic content which was indicated by the formation of a turquoise color after 5% FeCl3 reagent was added (in ethanol), phenolic content was 0.0727 mg GAE/g dry fraction, indicating antioxidant activity (IC50) amounted to 54.437 including strong categories as antioxidants and the results of GC–MS analysis obtained various kinds of compounds and it is suspected that compounds that provide potential as antioxidants are phytol. CONCLUSION: The bioactive compound of ethyl acetate fraction of seri (M. calabura) leaves contained phenolic components and has strong antioxidant activity.

Phytochemical Screening and Formulation of Hand Sanitizer Gel Preparations from Temu Hitam Rhizome Extract (Curcuma aeruginosa Roxb)

“…A total of 1000 grams of black turmeric simplicia was soaked in 96% ethanol in a ratio of 1 part simplicia dissolved in 10 parts solvent for 5 days using 75 parts of the 96% ethanol solvent. Then filtered, remaceration was carried out using 25 parts of 96% ethanol solvent for 2 days, then filtered, concentrated with a rotary evaporator at 40ᵒC [11], [12].…”

Phytochemical Screening and Antibacterial Potential Ethanol Extract of Kemangi Leaves (Ocimum basilicum L.) Towards Staphylococcus aureus

“…Ethanol Extract Preparation of Kemangi Leaves Kemangi leves extract was obtained by cold extraction,were maceration using 96% ethanol solvent, where 500 g of dried powder of kemangi leaves was soaked with 75 parts of ethanol for 5 days at least 3 times, stirring, then remaseration with 25 parts of the remaining solvent for 2 days, filtered and then evaporated with a rotary evaporator at 40-60ᵒC [11].…”

Antibacterial Activity Ethanolic Extract of Ocimum basilicum L. Leaves in Inhibiting the Growth of Escherichia coli and Pseudomonas aeruginosa

“…The method used in this test is the Agar Diffusion Method (Kirby-Bauer) 12 . A total of 0.1 mL of the bacterial suspension was pipetted and placed in a sterile petri dish, and then 15 mL of MHA medium was added 14,15 . Pour into a Petri dish and homogenize; place on the surface of the disc of paper media that contains each concentration, and use chloramphenicol antibiotic disc paper containing 30 mcg.…”