Determination of trimethoprim, sulphamethoxazole and its N4-acetyl metabolite in biological fluids by high-performance liquid chromatography

Rapid and simultaneous determination of sulfamethoxazole and trimethoprim in human plasma by high-performance liquid chromatography

“…To our knowledge, none of the previous investigators have been reported the effects of temperature on the retention of SMX and TMP, and most of the separations have been done on ambient temperature [2][3][4][5][6][7][8][9][10][11][12]. Interestingly, we found that temperature had a dual effect on the retention of TMP and SMX (Fig.…”

“…Simultaneous determination of SMX and TMP in biological fluids by high-performance liquid chromatography has been the subject of several investigations [2][3][4][5][6][7][8][9][10]. In spite of structure dissimilarity between SMX and TMP, organic solvent [2,3] or solid-phase [4,5] extraction have been developed but they are time-consuming and may show variable recoveries at different concentrations [3,5].…”

“…In spite of structure dissimilarity between SMX and TMP, organic solvent [2,3] or solid-phase [4,5] extraction have been developed but they are time-consuming and may show variable recoveries at different concentrations [3,5]. A column-switching method [6] has the problem of the availability of the required instruments.…”

Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (Artemia spp.) used for prophylactic chemotherapy of fish

“…Still, the remaining extract had to be substantially purified, particularly in view of the use of a nonselective detection wavelength. To this end, double-phase extraction, which has been used in connection with HPLC determination of trimethoprim and sulfamethoxazole in plasma (3,4,16,18) and trimethoprim alone in fish (5), was initially tried. However, the impure nature of Artemia extracts as well as the failure of ethyl acetate to efficiently extract trimethoprim, unlike sulfamethoxazole, at a pH of <7 led to the dismissal of this approach.…”

Development and validation of an HPLC method for simultaneous determination of trimethoprim and sulfamethoxazole in human plasma

“…Several high pressure liquid chromatography (HPLC) methods have been described for simultaneous determination of TMP and SMX in pharmaceutical or biological samples (Vree et al 1978;Bury and Mashford 1979;Spreux-Varoquaux et al 1983;Weber et al 1983;van der Steuijt and Sonneveld 1987;DeAngelis et al 1990;Laizure et al 1990;Ronn et al 1999;Pereira and Cass 2005). All these methods differ with respect to the mobile phase, flow rate, columns and detection methods.…”