Determination of uric acid and creatinine in human urine using hydrophilic interaction chromatography

Development of Zwitterionic Hydrophilic Liquid Chromatography (ZICⓇHILIC-MS) Metabolomics Method for Shotgun Analysis of Human Urine

“…The compounds elute in the order of decreasing hydrophobicity, in agreement with generally observed HILIC behaviour [23]. Besides the common partition mechanism in HILIC chromatography, the electrostatic interactions between charged analytes and the stationary phase plays a major part in the separation of creatinine [24]. The hydrophilic interaction and the ionic interaction were kept constant as the same pH buffer was maintained in both the mobile phase solvents and thus, the variation of the retention mainly depended on the variation of the charge state of both the analytes and the stationary phase.…”

Electrochemical behavior of isoproterenol in the presence of uric acid and folic acid at a carbon paste electrode modified with 2,7-bis(ferrocenyl ethyl)fluoren-9-one and carbon nanotubes

“…Therefore, the determination of UA with a simple method is essential because it serves as a marker for the detection of the above diseases. Comparing with other technologies, electrochemical method is more desirable because of its convenience and low cost [13][14][15][16][17].…”

Rapid and highly-sensitive uric acid sensing based on enzymatic catalysis-induced upconversion inner filter effect

“…Despite tremendous efforts was made to detection of UA with high selectivity and sensitivity (Dai et al, 2007;Zuo et al, 2011;Huang et al, 2014;Wang et al, 2014;Arora et al, 2009;Wang et al, 2012;Bera et al, 2011;Ballesta-Claver et al, 2013;Yao et al, 2003;Öhman, 1979), these methods still involved sophisticated equipment, complicated sample preparation processes, high cost, and time consuming immobilizing processes, which make them unsuitable for rapid detection and restrain their wide applications. The florescence method in detection of UA is fast, efficient and sensitive.…”