Determination of Urinary 6-Mercaptopurine and Three of its Metabolites by HPLC–UV Coupled with The Iodine–Azide Reaction

Zakrzewski, Robert; Borowczyk, Kamila; Łuczak, Adam; Młynarski, Wojciech; Trelińska, Joanna

doi:10.4155/bio.13.46

Cited by 13 publications

(1 citation statement)

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“…6-Mercaptopurine (6-MP), a sulfur analogue of adenine, is one of the main ingredients of anti-neoplastic agents, and uses its toxic properties to suppress cancers such as acute lymphoblastic leukemia and inammatory bowel diseases. 1,2 Different analytical methods have been proposed for the analysis of 6-MP, such as chemiluminescence, 3,4 electrochemical methods, [5][6][7] high performance liquid chromatography (HPLC), [8][9][10][11] mass spectrometry, 12 capillary electrophoresis (CE) 13 Raman assays, 14 and electrochemical assays. 5,15 The electrochemical determination of 6-MP has limited application in real samples due to the poor repeatability and complex electrode modication process.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence quenching and spectrophotometric methods for the determination of 6-mercaptopurine based on carbon dots

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Fluorescence quenching and spectrophotometric methods for the determination of 6-mercaptopurine based on carbon dots

et al. 2016

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Preparation of strongly fluorescent water-soluble dithiothreitol modified gold nanoclusters coated with carboxychitosan, and their application to fluorometric determination of the immunosuppressive 6-mercaptopurine

et al. 2018

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Water-soluble and non-aggregating gold nanoclusters (AuNCs) were obtained by modification of the AuNCs with dithiothreitol (DTT) and then coating them with carboxylated chitosan. This process remarkably enhances the dispersibility of DTT-coated AuNCs in water. The resulting AuNCs, on photoexcitation at 285 nm, display strong red emission with a maximum at 650 nm and a 23% quantum yield. Fluorescence is strongly and selectively suppressed in the presence of 6-mercaptopurine (6-MP). Photoluminescence drops linearly in the 0.1-100 μM 6-MP concentration range, and the detection limit of this assay is 0.1 μM. Other features of the modified AuNCs include a decay time of 8.56 μs, a 365 nm Stokes shift, good colloidal stability, ease of chemical modification, and low toxicity. Conceivably, these NCs may find a range of applications in biological imaging and optical sensing. Graphical abstract Highly fluorescent and water-soluble gold nanoclusters (AuNCs) were obtained by modification of the AuNCs with dithiothreitol (DTT) and then coating them with carboxylated chitosan (CC). The resulting CC/DTT-AuNCs were used for sensitive and selective detection of 6-mercaptopurine.

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An enzymatic ratiometric fluorescence assay for 6-mercaptopurine by using MoS2 quantum dots

Zhang

Liu

2018

Microchim Acta

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Determination of Urinary 6-Mercaptopurine and Three of its Metabolites by HPLC–UV Coupled with The Iodine–Azide Reaction

Abstract: The elaborated method was successfully applied for detection and quantitation of 6-MP and its selected metabolites in patients' urine samples.

Cited by 13 publications

References 8 publications

Fluorescence quenching and spectrophotometric methods for the determination of 6-mercaptopurine based on carbon dots

Fluorescence quenching and spectrophotometric methods for the determination of 6-mercaptopurine based on carbon dots

Preparation of strongly fluorescent water-soluble dithiothreitol modified gold nanoclusters coated with carboxychitosan, and their application to fluorometric determination of the immunosuppressive 6-mercaptopurine

An enzymatic ratiometric fluorescence assay for 6-mercaptopurine by using MoS2 quantum dots

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