Determination of Urinary 8-Hydroxydeoxyguanosine by Coupled-Column High-Performance Liquid Chromatography with Electrochemical Detection: A Noninvasive Assay for in Vivo Oxidative DNA Damage in Humans

Tagesson, Christer; Källberg, Magnus; Leanderson, Per

doi:10.3109/15376519109050855

Cited by 36 publications

(12 citation statements)

References 21 publications

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“…Separation of the analyte from the complex constituents of the urinary matrix represents a major challenge in the development of accurate methods. Therefore, many variant puriWcation procedures have been described for HPLC techniques: e.g., solid phase extraction (Shigenaga et al 1989), coupled-column HPLC (Loft et al 1992;Tagesson et al 1992;Kasai 2003), use of carbon columns (Bogdanov et al 1999) or immunoaYnity isolation of 8-OHdG (Degan et al 1991). In addition, solid phase extraction and/or HPLC have been used for clean-up procedure prior to GC-MS analysis of urinary 8-OHdG (Teixeira et al 1995;Holmberg et al 1999).…”

Section: Determination Of 8-ohdg In Urinementioning

confidence: 99%

8-Hydroxy-2′-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures

Pilger

Rüdiger

2006

Int Arch Occup Environ Health

300

204

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Oxidative DNA damage is considered to play an important role in pathophysiological processes, ageing and cancer. So far major interest has been on measuring 8-hydroxy-2'-deoxyguanosine (8-OHdG), the preferred methods relying on HPLC or GC-mass spectrometry. The high biological relevance of 8-OHdG is due to its ability to induce G-->T transversions, which are among the most frequent somatic mutations found in human cancers. Effects of workplace exposures on the level of white blood cell 8-OHdG or urinary 8-OHdG have been reported with controversial results. Exposures examined include asbestos, azo-dyes, benzene, fine particulate matter (PM(2.5)), glassworks, polycyclic aromatic hydrocarbons (PAHs), rubber manufacturing, silica, metals, styrene, toluene and xylenes. The available data indicate that there is still a lack of well established dose-response relations between occupational or environmental exposures and the induction of 8-OHdG. Smoking has been most consistently identified as a confounder for 8-OHdG, but various occupational studies did not reveal higher levels of 8-OHdG in smokers. Despite the conflicting results, the reported studies show promise for 8-OHdG as a biomarker of oxidative stress associated with chemical exposure. However, there are critical aspects related to the analytical challenge, artifactual production of 8-OHdG, inter- and intra-individual variation, confounding factors and inter-laboratory differences, implying that further work is needed to reach a consensus on the background level of 8-OHdG.

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Section: Determination Of 8-ohdg In Urinementioning

confidence: 99%

8-Hydroxy-2′-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures

Pilger

Rüdiger

2006

Int Arch Occup Environ Health

300

204

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“…The adduct can be determined at high precision and selectivity by electrochemical detection following separation of enzymatic DNA hydrolysate. using reverse-phase HPLC (21).…”

Section: Indulski and Lutz Molecular Epidemiology In Cancer Risk Assessmentioning

confidence: 99%

Molecular Epidemiology in Cancer Risk Assessment

Indulski¹,

Lutz²

1997

ACS Symposium Series

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The rapid development of molecular biology techniques has significantly aided the assessment of the detrimental consequences of chemically polluted environment on humans. It has also resulted in the development of molecular epidemiology, a new branch of epidemiology. Molecular epidemiology has become an important science which finds increasing applications in environmental monitoring. Nevertheless, a number of problems have emerged which seriously limit its practical application in epidemiologic research. Correct interpretation of test results seems to be one of the most difficult stages of assessing, by means of molecular epidemiology techniques, exposures to environmental pollutants and the resultant health effects. This refers in particular to those instances of the assessment of exposures (to environmental carcinogens for instance) for which formal exposure limits are not available. In many instances, molecular epidemiology tests are performed only because specified methods are available, but in many cases the results fail to provide any useful information. Also, the implementation of these new research methods has given rise to completely new ethical, social and legal problems. This is particularly true about research on the individual susceptibility to the adverse effects of environmental chemicals. And finally, it is extremely important to ensure that the enthusiasm about the feasibility of using the most recent molecular biology technologies does not overshadow the epidemiological problems associated with the necessity of providing suitable health care to the inhabitants of chemically polluted areas.Cancer is a long-lasting multi-stage process, and the epidemiologic data indicate that environmental chemical agents (chemical carcinogens) play an important role in this process. A chain of certain events must take place prior to carcinogenic cell transformation. The events include contact of the organism with the carcinogen, its absorption into the organism, transportation into the cells, transformation to a reactive metabolite (not all carcinogens become necessarily activated), and interaction with the critical (DNA) molecule in the target cells. The interaction results in the formation of carcinogen-DNA adducts, which in turn may lead to point mutations or breaks and/or rearrangements in the chromosomes. The changes in cellular genome DNA are not random. Usually specific genes or gene groups, which participate in the process

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“…ment of cancer. Automated analytical methods have been developed to measure 8-OHdG in human cells (13) or urine (14). 8-OHdG measured in cell DNA represents a steady-state level (a balance between DNA damage and cellular repair), whereas the exact source of urinary excre?…”

mentioning

confidence: 99%

Exposure to Indoor Background Radiation and Urinary Concentrations of 8-Hydroxydeoxyguanosine, a Marker of Oxidative DNA Damage

Sperati¹,

Abeni²,

Tagesson³

et al. 1999

Environmental Health Perspectives

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Determination of Urinary 8-Hydroxydeoxyguanosine by Coupled-Column High-Performance Liquid Chromatography with Electrochemical Detection: A Noninvasive Assay for in Vivo Oxidative DNA Damage in Humans

Cited by 36 publications

References 21 publications

8-Hydroxy-2′-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures

8-Hydroxy-2′-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures

Molecular Epidemiology in Cancer Risk Assessment

Exposure to Indoor Background Radiation and Urinary Concentrations of 8-Hydroxydeoxyguanosine, a Marker of Oxidative DNA Damage

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