Determination of Vancomycin Content in Dried Blood Spots for Therapeutic Drug Monitoring

Al-Ghazawi, Mutasim; Daoud, Nour El-Huda; Hadidi, K.; Alzweiri, Muhammed; AbuRuz, Salah

doi:10.32383/appdr/132021

Cited by 4 publications

(2 citation statements)

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“…Table 1 presents the details of the precision and accuracy data at the tested levels. The values obtained for accuracy of the developed method are in accordance with similar recent publications (Al‐Ghazawi et al, 2021; Scribel et al, 2020; Tariq et al, 2015).…”

Section: Resultssupporting

confidence: 90%

Development and validation of a dried plasma spot LC–MS/MS method for therapeutic monitoring of vancomycin and comparison with enzyme‐multiplied immunoassay

Vidal

Ossanes

Birk

et al. 2023

Biomedical Chromatography

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Vancomycin is used as an antimicrobial agent for the treatment of severe grampositive infections. The importance of therapeutic monitoring of antimicrobials has led to the development of more specific sample preparation techniques capable of identifying with accuracy the concentration of this substance in the organism. An aliquot of 10 μl of plasma was transferred to Whatman 903 paper and dried at room temperature. The extraction method was performed by cutting and transferring the paper to a microtube and adding sodium phosphate buffer and internal standard. The mixture was shaken and centrifuged, and a 5-μl aliquot was injected into the analytical system. The optimization of the main parameters that can influence the extraction efficiency was performed using multivariate approaches to obtain the best conditions. The method developed was validated, providing coefficients of determination higher than 0.994 and a lower limit of quantification of 1 mg/L. Within-and between-run precision ranged from 11.4 to 17.30% and from 6.65 to 13.51%, respectively. This method was successfully applied to 75 samples of patients undergoing vancomycin therapy. The method was rapid, simple, and environmentally friendly with satisfactory analytical performance and was advantageous over the laborious and time-consuming methodologies used in therapeutic drug monitoring routine analyses.

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Section: Resultssupporting

confidence: 90%

Development and validation of a dried plasma spot LC–MS/MS method for therapeutic monitoring of vancomycin and comparison with enzyme‐multiplied immunoassay

Vidal

Ossanes

Birk

et al. 2023

Biomedical Chromatography

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“…Однако для успешного проведения ТЛМ необходимо применение новых валидированных методик и технологий, знание новых физико-химических подходов к пробоподготовке биообразцов и наличие современного оборудования. В связи с этим актуальной задачей для исследований ТЛМ ванкомицина является разработка и валидация новых физикохимических методов анализа лекарственных препаратов [3,4,5].…”

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Development and Validation of a Quantitative HPLC/MS/MS Method for the Determination of Vancomycin in Blood Plasma

et al. 2022

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Within the framework of this study, a quantitative HPLC-MS/MS (high-performance liquid chromatography with mass spectrometric detection) method for the determination of vancomycin was developed. The method for the quantitative determination of vancomycin in human plasma was validated using the chromato-mass spectrometric method. The method was validated in accordance with the requirements of the EAEU for bioanalytical methods. The validated analytical range of the technique was 1-100 pg/mL in plasma.

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Adaptation of “Dried Blood Drop” Method for Therapeutic Drug Monitoring

Петров

Аникеев

Zayachnikova

et al. 2022

Farm. farmakol. (Pâtigorsk)

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To control the concentration of drugs with a narrow therapeutic range, and to conduct effective and safe treatments, Therapeutic Drug Monitoring (TDM) is carried out. However, to date, the implementation of TDM is associated with various difficulties, for the solution of which more convenient and less invasive methods for collecting biological material are being developed.The aim of the study was to develop protocols for the collection and storage of “dried blood spot” (DBS) samples, as well as protocols for the validation methods for the quantitative determination of drugs in whole blood, using this technology for subsequent therapeutic drug monitoring.Materials and methods. To analyze a “dried blood spot” method in detail and to identify the characteristic features of taking and storing biosamples, a collection and analysis of scientific literature over the past 10 years has been conducted. The search for literature materials has been carried out from open and accessible sources located in the scientific libraries of institutions, in electronic databases and search engines: Elibrary, PubMed, Scopus, Cyberleninka, Medline, ScienceDirect, Web of Science, Google Scholar. Primary protocols for taking, storing and analyzing samples of the “dried blood drop” have been prepared. To obtain the adequate quality samples, the developed protocols have been tested and optimized at the stages of selection and storage. By high-performance liquid chromatography with mass spectrometric detection (HPLC-MS/MS), using a “dried blood drop” as a sample preparation, drug validation protocols have been optimized to ensure that acceptable validation characteristics were achieved, and subsequent Therapeutic Drug Monitoring was performed.Results. The features of the collection, storage and analysis of the “dried blood spot” samples have been revealed. Such characteristics as a spot volume effect, a hematocrit effect, a droplet uniformity, which can affect the results of a quantitative HPLC-MS/MS analysis, have been determined. For a successful use of the new methods, appropriate protocols for taking samples of “dried blood spot” from the finger of adult patients and from the heel of newborns, as well as protocols for validating methods for the quantitative determination of drugs from these samples, have been developed.Conclusion. The application of the “dried blood spot” method using newly developed protocols for taking, storing and analyzing biological samples, relieves the existing constraints in conducting TDM, and can later become a promising method for conducting preclinical and clinical studies.

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Determination of Vancomycin Content in Dried Blood Spots for Therapeutic Drug Monitoring

Cited by 4 publications

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Development and validation of a dried plasma spot LC–MS/MS method for therapeutic monitoring of vancomycin and comparison with enzyme‐multiplied immunoassay

Development and validation of a dried plasma spot LC–MS/MS method for therapeutic monitoring of vancomycin and comparison with enzyme‐multiplied immunoassay

Development and Validation of a Quantitative HPLC/MS/MS Method for the Determination of Vancomycin in Blood Plasma

Adaptation of “Dried Blood Drop” Method for Therapeutic Drug Monitoring

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