Determination of Vitamin A and its Metabolites in Rat Testis: Possible Involvement of Vitamin A in Testicular Toxicity Caused by Molinate

Abstract

This study was conducted to evaluate the effect of molinate on retinoids homeostasis in rat testis. Molinate was administrated to male Sprague–Dawley rats (200 mg kg−1 in corn oil, ip). Retinoid measurements were made at 6, 12, 48 and 168 h time points after administration. Testis levels of retinoic acid decreased (32 %) in a statistically significant manner at the 12 and 48 h time points. However, retinol and retinaldehyde were not significantly affected by molinate. These results suggest that molinate affects retinoic acid synthesis in testis and could contribute to understanding the molecular mechanism of molinate involved testicular toxicity.

Protective Effect of All-Trans Retinoic Acid in Cisplatin-Induced Testicular Damage in Rats

“…Retinoic acid captures free radicals without interacting with biological targets and prevents free radical formation with its chain-breaking effect. Retinoic acid prevents lipid peroxidation in cellular membranes [8], and also has anti-proliferative, anti-inflammatory, and anti-cancer effects [9].…”

Prediction of embryotoxic potential using the ReProGlo stem cell-based Wnt reporter assay

“…There are no reports of molinate being a developmental toxicant, though it is known to cause testicular toxicity and sperm damage and thus to impair male fertility [17]. It has been suggested that molinate acts by inhibiting retinoic acid synthesis [18]. Retinoic acid itself activates the Wnt signaling pathway in our assay while molinate produced an inhibitory effect.…”