Determination of α-methyldopa in human plasma by validated high-performance liquid chromatography with fluorescence detection

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

“…As Tabelas 4 e 5 relacionam os fármacos que têm sido quantificados empregando métodos de separação como CLAE [161][162][163][164][165][166][167][168][169][170][171][172][173][174][175][176][177] (Tabela 4) e eletroforese capilar 50,[178][179][180][181] (Tabela 5) na análise de amostras complexas, como plasma e urina, ou na quantificação de misturas de fármacos e em estudos relacionados com bioequiva-lência, farmacocinêtica e biodisponibilidade. Desta forma, estas técnicas apresentam grande potencialidade de uso em situações específicas onde a matriz da amostra seja muito complexa.…”

Differential pulse voltammetric determination of methyldopa using MWCNTs modified glassy carbon decorated with NiFe2O4 nanoparticles

“…Methyldopa is a competitive inhibitor of dopadecarboxylase, which metabolized Ldopa into dopamine [3,4]. Many analytical methods have been reported for determination of catecholamine drugs, such as methyldopa, in biological samples including spectrophotometry [5][6][7][8], fluorescence [9,10], high-performance liquid chromatography [11][12][13], chemiluminescence [14][15][16], gas chromatography [17], and potentiometry [18]. Some of these methods described above have disadvantages such as low sensitivity, long analysis times, and/or require expensive instruments.…”

A rapid high performance liquid chromatographic determination of methyldopa in human serum with fluorescence detection and alumina extraction: Application to a bioequivalence study

“…phase extraction [6], protein precipitation [5,7,8] or a tedious, time consuming alumina adsorption [10,11]. Different run time of analysis between the range of 5.5-35 [5,6] min and various quantification limits (20 ng/ml [5], 10 ng/ml [6], 0.8 g/ml [7], 50 ng/ml [9], 8 g/ml [10], 10 ng/ml [11]) using different volumes of injection (40 l [5], 100 l [6,11], 20 l [7,9], 1 l [10]) and samples (200 l [5], 1 ml [6][7][8][9]11], 30 ml [10]) have been reported for analysis of the drug in biological fluids. Analytical conditions in the published methods consisted of gradient [6] or isocratic elution of mobile phase with [6,7] or without using of an ion pair agent.…”

“…Numerous analytical techniques including spectrofluorimetric [2][3][4] and chromatographic [5][6][7][8][9][10][11] methods are now available for analysis of methyldopa in biological fluids. Urinary and plasma concentration of the drug most commonly have been measured by high performance liquid chromatography (HPLC) using electrochemical [7][8][9]11], mass [5], fluorescence [6] or UV [11] detections. Sample preparation in most of these methods achieved by solid * Corresponding author.…”