A frequently encountered problem in signal processing is harmonic retrieval in additive colored Gaussian or non-Gaussian noise, especially when the frequencies of the harmonic signals are very close in space. The purpose of this paper is to develop an efficient Blind Source Separation (BSS) algorithm from linear mixtures of source signals, which enables to separate harmonic source signals using only one observed channel signal even if the frequencies of the harmonic signals are closely spaced. First, we establish the BSS based harmonic retrieval model in additive noise by using the only one observed channel, and analyze the fundamental principle by utilizing BSS method to retrieve harmonics. Then, we propose a BSS-based approach to the harmonic retrieval by resorting the concept of W-disjoint orthogonality in the over-complete BSS situation, and as a result, we get the separation algorithm using only one channel mixed signals. Simulation results show that the proposed separation algorithm-BSS-HR is able to separate the harmonic source signals.