Development and application of a full-length infectious clone of potato virus Y isolate belonging to SYR-I strain

Cheng, De‐Jie; Tian, Yanping; Geng, Chao; Guo, Yushuang; Jia, Mengao; Li, Xiangdong

doi:10.1016/j.virusres.2019.197827

Cited by 15 publications

(13 citation statements)

References 32 publications

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“…Cross-protection is the phenomenon that plants infected with one mild strain of a virus will escape or prevent the infection by closely related severe viruses (Kunkel, 1955). It has been used for the control of more than 30 viruses including citrus tristeza virus (CTV), papaya ringspot virus (PRSV), potato virus X (PVX), potato virus Y (PVY), sugarcane mosaic virus (SCMV), and tobacco mosaic virus (TMV) in the glasshouses or the fields (Rast, 1972;Krstic et al, 1995;You et al, 2005;Folimonova, 2013;Cong et al, 2019;Cheng et al, 2020). Cross-protection is an environmentally safe and friendly disease management strategy and meets the societal demand for high-quality and sustainable agricultural production systems (Pechinger et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

A Spontaneous Complementary Mutation Restores the RNA Silencing Suppression Activity of HC-Pro and the Virulence of Sugarcane Mosaic Virus

Cheng

et al. 2020

Front. Plant Sci.

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Cross-protection is a promising measure to control plant viral diseases. Reverse genetics had been recently adopted to generate attenuated mutants that have potential in crossprotection. But studies on the variability of the progeny viruses of the attenuated mutants are scarce. Sugarcane mosaic virus (SCMV; genus Potyvirus, family Potyviridae) is the prevalent virus inducing maize dwarf mosaic disease in China. Here, we showed that the substitution of arginine with isoleucine in the FRNK motif at position 184 of helper component-proteinase (HC-Pro) abolished its RNA silencing suppression (RSS) activity, drastically reduced the virulence and accumulation level of SCMV, and impaired the synergism between SCMV and maize chlorotic mottle virus. The attenuated mutant could protect maize plants from a severe infection of SCMV. However, a spontaneous mutation of glycine at position 440 to arginine in HC-Pro rescued the virulence and synergism with maize chlorotic mottle virus of SCMV and the RSS activity of HC-Pro. Similar results were obtained with tobacco vein banding mosaic virus and watermelon mosaic virus. These results provide novel evidence for the complementary mutation of potyviruses in maintaining the HC-Pro RSS activity and potyviral virulence and remind us of evaluating the potential risk of attenuated mutants thoroughly before applying for the control of plant viral diseases via cross-protection.

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Section: Introductionmentioning

confidence: 99%

A Spontaneous Complementary Mutation Restores the RNA Silencing Suppression Activity of HC-Pro and the Virulence of Sugarcane Mosaic Virus

Cheng

et al. 2020

Front. Plant Sci.

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“…In the molecular arms race, potyvirus has evolved an effective RNA silencing suppressor (HC-Pro) to counteract the RNA silencing mechanism in plants (Anandalakshmi et al, 1998;Ivanov et al, 2016;Valli et al, 2018). Helper component-proteinase is a major virulence determinant of potyviruses and an important candidate for screening attenuated mutants (Seo et al, 2011;Huang et al, 2019;Bao et al, 2020;Cheng et al, 2020). Single or multiple amino acid mutations in the conserved motifs of HC-Pro affected potyviral virulence (Desbiez et al, 2010;Kung et al, 2014;Tuo et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Cross-protection is an environmentally safe method to control plant viral diseases (Pechinger et al, 2019) and has been used to control multiple plant viruses in the laboratory or field (Rast, 1972;Krstic et al, 1995;Cong et al, 2019;Cheng et al, 2020). Mild viruses can protect plants from subsequent infection of the same or closely related severe strains (Zhang and Qu, 2016;Zhang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain

Zhu

Jiang

et al. 2021

Front. Plant Sci.

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Sugarcane mosaic virus (SCMV; genus Potyvirus) induces maize dwarf mosaic disease that has caused serious yield losses of maize in China. Cross-protection is one of the efficient strategies to fight against severe virus strains. Although many mild strains have been identified, the spontaneous mutation is one of the challenging problems affecting their application in cross-protection. In this study, we found that the substitution of cysteine (C) at positions 57 or 60 in the zinc finger-like motif of HC-Pro with alanine (A; C57A or C60A) significantly reduced its RNA silencing suppression activity and SCMV virulence. To reduce the risk of mild strains mutating to virulent ones by reverse or complementary mutations, we obtained attenuated SCMV mutants with double-mutations in the zinc finger-like and FRNK motifs of HC-Pro and evaluated their potential application in cross-protection. The results showed that the maize plants infected with FKNK/C60A double-mutant showed symptomless until 95 days post-inoculation and FKNK/C60A cross-protected plants displayed high resistance to severe SCMV strain. This study provides theoretical and material bases for the control of SCMV through cross-protection.

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“…Cloned viruses expressing fluorescent reporters are widely used in molecular biology (Cheng et al, 2020; Mei et al, 2019; Wang et al, 2020; Xie et al, 2020). These viruses have been used to assess virus replication in a range of cells, tissues, and organisms, greatly advancing our understanding of virus biology and virus‐host interactions.…”

Section: Introductionmentioning

confidence: 99%

Beeporter: Tools for high‐throughput analyses of pollinator‐virus infections

Evans

Banmeke

Palmer‐Young

et al. 2021

Molecular Ecology Resources

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Pollinators are in decline thanks to the combined stresses of disease, pesticides, habitat loss, and climate. Honey bees face numerous pests and pathogens but arguably none are as devastating as Deformed wing virus (DWV). Understanding host‐pathogen interactions and virulence of DWV in honey bees is slowed by the lack of cost‐effective high‐throughput screening methods for viral infection. Currently, analysis of virus infection in bees and their colonies is tedious, requiring a well‐equipped molecular biology laboratory and the use of hazardous chemicals. Here we describe virus clones tagged with green fluorescent protein (GFP) or nanoluciferase (nLuc) that provide high‐throughput detection and quantification of virus infections. GFP fluorescence is measured noninvasively in living bees via commonly available long‐wave UV light sources and a smartphone camera, or a standard ultraviolet transilluminator gel imaging system. Nonlethal monitoring with GFP allows continuous screening of virus growth and serves as a direct breeding tool for identifying honey bee parents with increased antiviral resistance. Expression using the nLuc reporter strongly correlates with virus infection levels and is especially sensitive. Using multiple reporters, it is also possible to visualize competition, differential virulence, and host tissue targeting by co‐occuring pathogens. Finally, it is possible to directly assess the risk of cross‐species “spillover” from honey bees to other pollinators and vice versa.

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Development and application of a full-length infectious clone of potato virus Y isolate belonging to SYR-I strain

Cited by 15 publications

References 32 publications

A Spontaneous Complementary Mutation Restores the RNA Silencing Suppression Activity of HC-Pro and the Virulence of Sugarcane Mosaic Virus

A Spontaneous Complementary Mutation Restores the RNA Silencing Suppression Activity of HC-Pro and the Virulence of Sugarcane Mosaic Virus

Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain

Beeporter: Tools for high‐throughput analyses of pollinator‐virus infections

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