2014
DOI: 10.1371/journal.pone.0085606
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Development and Application of an Immunoaffinity Column Enzyme Immunoassay for Mycotoxin Zearalenone in Complicated Samples

Abstract: The zearalenone (ZEA) monoclonal antibody (mAb) 2D3, one of the highest sensitivity antibodies, was developed. Based on this mAb, it was established of an immunoaffinity column (IAC) coupled with an indirect competitive enzyme-linked immunosorbent assay (icELISA). After optimization, the icELISA allowed an IC50 against ZEA of 0.02 µg L−1. The mAb 2D3 exhibited a high recognition of ZEA (100%) and β-zearalenol (β-ZOL, 88.2%). Its cross-reactivity with α-zearalenol (α-ZOL) and β-zearalanol (β-ZAL) were found to … Show more

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Cited by 34 publications
(20 citation statements)
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“…Nevertheless, the occurrence of structurally related mycotoxins or matrice interference can obstruct conjugate and antibody binding vulnerable to errors in quantifiable mycotoxin measurements. Tang et al () validated an indirect ELISA detection technique with an immunoaffinity column sample preparation using the same antibody used by Klarić, Cvetnić, Pepeljnjak, and Kosalec () and was found to be extremely sensitive at 0.02 μg/L. In order to recover high sensitivity, most researchers concentrated on modifying the normal ELISA protocol.…”
Section: Traditional Quantitative Methodsmentioning
confidence: 99%
“…Nevertheless, the occurrence of structurally related mycotoxins or matrice interference can obstruct conjugate and antibody binding vulnerable to errors in quantifiable mycotoxin measurements. Tang et al () validated an indirect ELISA detection technique with an immunoaffinity column sample preparation using the same antibody used by Klarić, Cvetnić, Pepeljnjak, and Kosalec () and was found to be extremely sensitive at 0.02 μg/L. In order to recover high sensitivity, most researchers concentrated on modifying the normal ELISA protocol.…”
Section: Traditional Quantitative Methodsmentioning
confidence: 99%
“…All inorganic chemicals and organic solvents were of reagent grade unless stated otherwise. Anti-ochratoxin A monoclonal antibody (mAb) 1H2 [19], anti-aflatoxin B1 mAb 1C11 [40], anti-zearalenone mAb 2D3 [41] and anti-deoxynivalenol mAb 1D5 were produced in our laboratory. Nanobody VHH 2-24 was obtained and purified by following our previous instructions.…”
Section: Methodsmentioning
confidence: 99%
“…ZEN–BSA conjugates and ZEN monoclonal antibody were prepared in our laboratory, the IC ELISA revealed an IC50 toward ZEN of 0.02 μg/L. Cross‐reactivity rates against ZEN, β‐ZOL, α‐ZOL and β‐ZAL were 100, 88.2, 4.4 and 4.6%, respectively . Sample pads, nitrocellulose (NC) membranes, and absorbent pads were purchased from Shanghai Jieyi Biotechnology Co. Ltd. (Shanghai China).…”
Section: Methodsmentioning
confidence: 99%