Development and application of immunochromatographic tests for the detection of staphylococcal enterotoxin E

Development of a Single-Reaction Multiplex PCR Toxin Typing Assay for Staphylococcus aureus Strains

“…In the SET-RPLA test, enterotoxin E is detected by crossreaction with the enterotoxin A-specific antibody, leading to ambiguous results. Recently, Lakner et al (26) have reported the development of a specific antibody-based test for the presence of SEE; however, this now means that five individual tests must be performed on each isolate to be toxin typed and, as the number of toxin types characterized increases, an increasing number of tests will be required for a full analysis of toxigenotype in the future. a Human isolates came from 21 cases of non-line scepticemia, 4 cases of line-associated scepticemia, and 14 healthy adults.…”

One-step detection of matured females using immunochromatography measuring kit for aquaculture of the sea cucumber Apostichopus japonicus (Selenka)

“…Except for the above higher-concentration samples, the present reaction time of the samples with a concentration lower than 4 g mL À1 is similar to those already on the market. These times include 20 min for a Staphylococcus aureus enterotoxin serotype E detection kit (Lakner et al, 1998), 15 min for poisonous algae Alexandrium minutum in shell fish farming (Gas et al, 2010), and 5-30 min for the detection of organo-phosphorus pesticides in agricultural farming (Hua et al, 2012) but slightly slower compared with that of the 3-10 min required for melamine detection in raw milk, milk products and animal feed (Li et al, 2011), 10 min for a Directigen EZ RSV detection kit (Kuroiwa et al, 2004) and a canine trypsin-like immunoreactivity (cTLI) test used in veterinary medicine (Takahashi et al, 2003).…”

“…The technology has been applied to detect pathogens in medical sciences (Seto and Gillam, 1994;Depierreux et al, 2000;Watanabe et al, 2001;Thongprachum et al, 2010), agriculture (Lakner et al, 1998;Li et al, 2011;Hua et al, 2012), veterinary medicine (Oh et al, 2006;Waritani et al, 2007) and fisheries (Takahashi et al, 2003;Adams and Thompson, 2008;Gas et al, 2010;Kum and Sekkin, 2011). The technique has been also applied to detect hormones, such as human chorionic gonadotropin in urine, for early detection of pregnancy (May, 1991).…”