Development and characterization of 14 microsatellites for the eastern chipmunk, Tamias striatus

Vandal, Katherine; Houle, Carolyne; Archambault, Annie; Réale, Denis; Garant, Dany

doi:10.1007/s11033-020-05586-1

Cited by 5 publications

(3 citation statements)

References 17 publications

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“…We conducted microsatellite genotyping using 23 loci previously employed in the study system (Bergeron, Réale, et al, 2011b; Chambers & Garant, 2010; Vandal et al, 2020). The detailed genotyping protocol, is described in Chambers and Garant (2010), Vandal et al (2020). The parentage assignment protocol is fully described in Bergeron, Réale, et al (2011b).…”

Section: Methodsmentioning

confidence: 99%

Telomere length positively correlates with pace‐of‐life in a sex‐ and cohort‐specific way and elongates with age in a wild mammal

et al. 2022

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Understanding ageing and the diversity of life histories is a cornerstone in biology. Telomeres, the protecting caps of chromosomes, are thought to be involved in ageing, cancer risks and life‐history strategies. They shorten with cell division and age in somatic tissues of most species, possibly limiting lifespan. The resource allocation trade‐off hypothesis predicts that short telomeres have thus coevolved with early reproduction, proactive behaviour and reduced lifespan, that is, a fast pace‐of‐life syndrome (POLS). Conversely, since short telomeres may also reduce the risks of cancer, the anticancer hypothesis advances that they should be associated with slow POLS. Conclusion on which hypothesis best supports the role of telomeres as mediators of life‐history strategies is hampered by a lack of study on wild short‐lived vertebrates, apart from birds. Using seven years of data on wild Eastern chipmunks Tamias striatus, we highlighted that telomeres elongate with age (n = 204 and n = 20) and do not limit lifespan in this species (n = 51). Furthermore, short telomeres correlated with a slow POLS in a sex‐specific way (n = 37). Females with short telomeres had a delayed age at first breeding and a lower fecundity rate than females with long telomeres, while we found no differences in males. Our findings support most predictions adapted from the anticancer hypothesis, but none of those from the resource allocation trade‐off hypothesis. Results are in line with an increasing body of evidence suggesting that other evolutionary forces than resource allocation trade‐offs shape the diversity of telomere length in adult somatic cells and the relationships between telomere length and life‐histories.

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Section: Methodsmentioning

confidence: 99%

Telomere length positively correlates with pace‐of‐life in a sex‐ and cohort‐specific way and elongates with age in a wild mammal

et al. 2022

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“…We extracted DNA from ear tissue and genotyped individuals at 11 polymorphic microsatellite loci for site 1 and 14 additional microsatellites for sites 2,3, and 4, using protocols developed for this species (see Chambers and Garant 2010; Vandal et al. 2020 for details). We first assigned maternity when we trapped juveniles at their emergence from the burrow.…”

Section: Methodsmentioning

confidence: 99%

Indirect genetic and environmental effects on behaviors, morphology, and life‐history traits in a wild Eastern chipmunk population

et al. 2021

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Additive genetic variance in a trait reflects its potential to respond to selection, which is key for adaptive evolution in the wild. Social interactions contribute to this genetic variation through indirect genetic effects—the effect of an individual's genotype on the expression of a trait in a conspecific. However, our understanding of the evolutionary importance of indirect genetic effects in the wild and of their strength relative to direct genetic effects is limited. In this study, we assessed how indirect genetic effects contribute to genetic variation of behavioral, morphological, and life‐history traits in a wild Eastern chipmunk population. We also compared the contribution of direct and indirect genetic effects to traits evolvabilities and related these effects to selection strength across traits. We implemented a novel approach integrating the spatial structure of social interactions in quantitative genetic analyses, and supported the reliability of our results with power analyses. We found indirect genetic effects for trappability and relative fecundity, little direct genetic effects in all traits and a large role for direct and indirect permanent environmental effects. Our study highlights the potential evolutionary role of social permanent environmental effects in shaping phenotypes of conspecifics through adaptive phenotypic plasticity.

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“…We extracted DNA from ear tissues using the salting out method of Aljanabi and Martinez (1997) adapted to eastern chipmunks (Chambers and Garant 2010). Individuals were genotyped at 23 microsatellites on sites 2-4 and at 11 loci on site 1 (Anderson et al 2007, Vandal et al 2020). We trapped juveniles as they emerged from their maternal burrows (5-7 weeks after birth) and carried out maternal assignment using the CERVUS 3.0.3 software (Kalinowski et al 2007(Kalinowski et al , 2010, which allowed us to corroborate the maternal identity and to assign the paternity.…”

Section: Genetic Materials and Litter Sizementioning

confidence: 99%

Age at first reproduction and senescence in a short‐lived wild mammal

Allain,

Tissier,

Bergeron

et al. 2023

Oikos

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Senescence is the degradation of biological functions with increasing age. Its existence and relationship with life‐history strategies remains poorly studied in short‐lived wild vertebrate species. We investigated the relationships between age at first reproduction (AFR), reproductive senescence and longevity in an eastern chipmunk Tamias striatus population, where the first opportunity to breed is conditioned by pulses of seed production by trees (i.e. masts). We used 11 years of data from a longitudinal study, in which females breed for the first time at seven, 15 or 22 months of age and males at seven or 15 months of age. We first assessed the effect of age on three traits associated with breeding performance, namely the number of offspring produced, and the probability of producing an offspring. We then tested whether an earlier AFR accelerated reproductive senescence and reduced survival of both males and females. We found sex‐specific relationships between AFR and senescence. Females reproducing at 15 or 22 months of age showed reproductive senescence, but early‐breeding females did not show any decline in reproductive performance at an older age. Also, although we observed reproductive senescence in males, it was not affected by AFR. Our results are consistent with studies highlighting the existence of reproductive senescence in small, wild mammals. Importantly, we provide the first evidence that AFR can strongly influence the patterns of senescence in small short‐lived species, and does it in a sex‐specific way. Our results highlight the importance of studying life‐history strategies in both males and females when studying senescence in the wild.

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Development and characterization of 14 microsatellites for the eastern chipmunk, Tamias striatus

Cited by 5 publications

References 17 publications

Telomere length positively correlates with pace‐of‐life in a sex‐ and cohort‐specific way and elongates with age in a wild mammal

Telomere length positively correlates with pace‐of‐life in a sex‐ and cohort‐specific way and elongates with age in a wild mammal

Indirect genetic and environmental effects on behaviors, morphology, and life‐history traits in a wild Eastern chipmunk population

Age at first reproduction and senescence in a short‐lived wild mammal

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