Development and monitoring of an integrated bioprocess for production of a potential malaria vaccine with Pichia pastoris

Borchert, Sven-Oliver; Voß, Tobias; Schuetzmeier, Fabian; Paul, Jessica; Cornelissen, Gesine; Luttmann, R.

doi:10.1016/j.jprocont.2015.08.006

Cited by 8 publications

(3 citation statements)

References 18 publications

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“…Daher wird die vorgelagerte Satzkultur‐Phase mit Glycerin als Substrat durchgeführt, das P. pastoris ebenfalls verstoffwechseln kann . Die Produktion des Zielproteins eGFP erfolgt daher erst in der Feeding‐Phase mit Zuführung von Methanol .…”

Section: Materials Und Methodenunclassified

Einsatz eines Online‐Methanol‐Messsystems zur Regelung von Zulaufsatzkultur‐Fermentationen

Schumbera

Schmüser

Hinderer

et al. 2020

Chemie Ingenieur Technik

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Zur Optimierung von Zulaufsatzkultur‐Fermentationen von methylotrophen Organismen wird eine Online‐Messmethode vorgestellt, mit der die Methanol‐Konzentration im Medium während einer Fermentation durch ein Spülgaspervaporations‐Prinzip bestimmt werden kann. Im Gegensatz zu anderen Analysemethoden bietet die Messmethode die Möglichkeit, die Substratkonzentration bei Prozessen mit Methanol als zentralem Substrat über eine Regelung auf einem definierten Wert zu halten. Es werden Schwierigkeiten, aber auch deren Überwindung bei der Adaption der Messmethode auf Fermentationsprozesse dargestellt.

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Section: Materials Und Methodenunclassified

Einsatz eines Online‐Methanol‐Messsystems zur Regelung von Zulaufsatzkultur‐Fermentationen

Schumbera

Schmüser

Hinderer

et al. 2020

Chemie Ingenieur Technik

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“…Moreover, a greener and sustainable process would reduce the consumption of water, energy, and associated raw material. It is common to replace original fed-batch culture with continuous ( Rahimi et al, 2019a ; Rahimi et al, 2019b ; Nieto-Taype et al, 2020 ) or semi-continuous ( Pfeffer et al, 2006 ; Borchert et al, 2015 ) culture.…”

Section: Introductionmentioning

confidence: 99%

Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation

Wang

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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The large-scale fermentation of Pichia pastoris for recombinant protein production would be time consuming and produce a large amount of waste yeast. Here we introduce a novel semi-continuous fermentation process for P. pastoris GS115 that can separate vitality cells from broth and recycle the cells to produce high-secretory recombinant pectate lyase. It is based on differences in cell sedimentation coefficients with the formation of salt bridges between metal ions and various cell states. Compared to batch-fed cultivation and general semi-continuous culture, the novel process has significant advantages, such as consuming fewer resources, taking less time, and producing less waste yeast. Sedimentation with the addition of Fe3+ metal ions consumed 14.8 ± 0.0% glycerol, 97.8 ± 1.3% methanol, 55.0 ± 0.9 inorganic salts, 81.5 ± 0.0% time cost, and 77.0 ± 0.1% waste yeast versus batch-fed cultivation to produce an equal amount of protein; in addition, the cost of solid–liquid separation was lower for cells in the collected fermentation broth. The process is economically and environmentally efficient for producing recombinant proteins.

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“…Approximately half of the world’s population lives under the threat of malaria (Borchert et al, 2015). According to 2018 World Malaria Report data, ∼ 228 million clinical cases of the disease were reported worldwide, of which 405,000 resulted in death.…”

Section: Introductionmentioning

confidence: 99%

Tetrahymena thermophila Granule Lattice Protein 3 Improves Solubility of Sexual Stage Malaria Antigens Expressed in Escherichia coli

Akkale

Cassidy-Hanley

Clark

2021

Preprint

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The requirement for low cost manufacturing makes bacterial cells a logical platform for the production of recombinant subunit vaccines for malaria. However, protein solubility has been a major stumbling block with prokaryotic expression systems. Notable examples include the transmission blocking vaccine candidates, Pfs25 and Pfs48/45, which are almost entirely insoluble when expressed as recombinant proteins in Escherichia coli. Various solubility tags have been used with limited success in improving solubility, although recent studies with granule lattice protein 1 (Grl1p) from the ciliated protozoan, Tetrahymena thermophila, have shown promise. Here, we examine a related solubility tag, granule lattice protein 3 (Grl3p) from T. thermophila, and compare it to both Grl1p and the well-studied maltose binding protein (MBP) used to improve the solubility of multiple protein targets. We find that Grl3p performs comparably to Grl1p when linked to Pfs25 but significantly improves solubility when paired with Pfs48/45.

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Development and monitoring of an integrated bioprocess for production of a potential malaria vaccine with Pichia pastoris

Cited by 8 publications

References 18 publications

Einsatz eines Online‐Methanol‐Messsystems zur Regelung von Zulaufsatzkultur‐Fermentationen

Einsatz eines Online‐Methanol‐Messsystems zur Regelung von Zulaufsatzkultur‐Fermentationen

Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation

Tetrahymena thermophila Granule Lattice Protein 3 Improves Solubility of Sexual Stage Malaria Antigens Expressed in Escherichia coli

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