Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn. by improved RAPD amplification and molecular cloning

Cheng, Jingliang; Long, Yan; Wei, Chunli; Fu, Shelly; Fu, Junjiang

doi:10.1016/j.ejbt.2014.11.004

Cited by 41 publications

(20 citation statements)

References 27 publications

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“…(Mei et al 2014), Litchi chinensis Sonn. (Cheng et al 2015). The result showed the RAPD patterns from each selected primer and total 35 loci, and 18 haplotype patterns were obtained (Fig.…”

Section: Resultsmentioning

confidence: 99%

Genetic differentiation and antioxidant activities ofBouea macrophyllaGriffith in Nakhon Nayok province

Thummajitsakul

Silprasit

2017

JABC

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Genetic differentiation and antioxidant activities in ethanolic extracts from leaves of Bouea macrophylla Griffith were determined. The result revealed genetic differentiation among sour ma-praang, ma-yong and sweet ma-praang of B. macrophylla Griffith (φ PT =0.772, p-value=0.000). In addition, high genetic diversities were found in sour ma-praang and sweet ma-praang populations (P: 51.4 and 57.1 %; He: 0.1900.035 and 0.2240.036, respectively), but low genetic diversity was found in ma-yong population (P: 8.6 %; He: 0.0350.021). Total phenolic contents of sour ma-praang, ma-yong and sweet ma-praang were estimated as 680.51±89.81, 701.03±59.89 and 530.85±41.23 mg gallic acid/g extract, respectively. Free radical scavenging activities of sour mapraang, ma-yong and sweet ma-praang were found (1/EC50= 4.17, 1.43 and 1.37, respectively) corresponding with metalchelating activities (1/EC50=0.83, 0.65 and 0.17, respectively). Therefore, the obtained data may be applied to cultivation and utilization of their leaves as source of natural phenolics and antioxidants.

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“…(Mei et al 2014), Litchi chinensis Sonn. (Cheng et al 2015). The result showed the RAPD patterns from each selected primer and total 35 loci, and 18 haplotype patterns were obtained (Fig.…”

Section: Resultsmentioning

confidence: 99%

Genetic differentiation and antioxidant activities ofBouea macrophyllaGriffith in Nakhon Nayok province

Thummajitsakul

Silprasit

2017

JABC

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“…To overcome this problem, it is necessary to develop co-dominant markers from dominant markers to avoid lengthy RAPD reaction (Li et al 2010). Such markers are typical oligonucleotides designed from the sequences of the amplicon of RAPD, and they mostly have a high probability of producing polymorphic amplicons (Cheng et al 2015). Yang et al (2008) isolated 38 strains of bacteria from black liquor and 11 PCR-DNA profiles.…”

Section: Random Amplified Polymorphic Dna (Rapd)mentioning

confidence: 99%

Review of Molecular Techniques for the Identification of Bacterial Communities in Biological Effluent Treatment Facilities at Pulp and Paper Mills

et al. 2017

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One of the processes most used in biotechnology today for handling industrial liquid wastes is biological wastewater treatment. The efficiency and quality of its operation depends on the composition and activity of the microbial community that is present. The application of traditional and molecular techniques has provided a glimpse into the "black box" and has given information to improve the wastewater treatment process. However, bleach pulp and paper mill effluents require a better understanding of the active bacterial population. For the study of these microorganisms, molecular techniques have been used for more than 15 years. However, there has been a lack of knowledge of the physiological requirements and relations with the environment, which seems to be very difficult to obtain involving profile on the diversity. Nowadays, highthroughput sequencing technology is a promising method that makes it possible to identify the entire profile of microbial communities. In combination with fingerprint methods, this approach allows the identification and analysis of the whole biodiversity of microbial communities. In this review, several identification techniques will be discussed.

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“…These methods have been effectively used for the identification of herbal medicines and dietary supplements and have facilitated the development of specific, sensitive, simple, and reproducible genetic markers for this purpose (Torelli et al, 2014;Xin et al, 2014;Cheng et al, 2015;Coutinho Moraes et al, 2015). In particular, RAPD analysis is one of the most effective methods to detect high levels of polymorphism, with many advantages including high speed, low cost, and a requirement for only small quantities of template DNA (and consequently plant material).…”

Section: Introductionmentioning

confidence: 96%

Development of molecular markers for authentication of the medicinal plant species Patrinia by random amplified polymorphic DNA (RAPD) analysis and multiplex-PCR

Moon

Lee

Kim

et al. 2016

Hortic. Environ. Biotechnol.

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The authentication of components from plant species is vital for the quality control and standardization of herbal medicines. Due to morphological similarities when dried, it is very difficult to distinguish authentic medicinal plants Patrinia villosa and P. scabiosifolia from adulterants of P. rupestris and P. saniculifolia in the herbal medicine Patriniae Radix. To establish a reliable authentication tool distinguishing authentic Patriniae Radix species from adulterants, we used random amplified polymorphic DNA (RAPD) genomic profiling and obtained several species-specific DNA fragments for each of the four Patrinia species. Based on the sequences of these amplicons, we developed sequence characterized amplified region (SCAR) markers as stable molecular authentication tools. SCAR markers to identify the authentic Patriniae Radix species, P. villosa and P. scabiosifolia, were developed. To accurately identify adulterants, we also developed SCAR markers able to identify the inauthentic P. rupestris and P. saniculifolia species. Furthermore, we established multiplex-PCR SCAR assays to simultaneously distinguish all four species using combinations of species-specific SCAR marker primer sets. These efforts resulted in reliable DNA-based molecular markers to identify authentic Patriniae Radix species from their adulterants and a rapid authentication assay for the efficient identification of Patrinia species. These markers will facilitate the standardization of Patriniae Radix preparations and therefore prevent the distribution of adulterants.

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Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn. by improved RAPD amplification and molecular cloning

Cited by 41 publications

References 27 publications

Genetic differentiation and antioxidant activities ofBouea macrophyllaGriffith in Nakhon Nayok province

Genetic differentiation and antioxidant activities ofBouea macrophyllaGriffith in Nakhon Nayok province

Review of Molecular Techniques for the Identification of Bacterial Communities in Biological Effluent Treatment Facilities at Pulp and Paper Mills

Development of molecular markers for authentication of the medicinal plant species Patrinia by random amplified polymorphic DNA (RAPD) analysis and multiplex-PCR

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