Development and validation of a biosensor-based immunoassay for progesterone in bovine milk

Gillis, Els H; Gosling, James P.; Sreenan, J.M.; Kane, Marian

doi:10.1016/s0022-1759(02)00166-7

Cited by 80 publications

(37 citation statements)

References 22 publications

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“…Simultaneous immobilisation of OA onto all flow cells of the CM5 sensor chip was performed following modifications of a previously described method (Gillis et al, 2002). For covalent immobilisation, carboxyl groups of the sensor surface were activated by derivatisation with NHS mediated by EDC.…”

Section: Immobilisation Of Oa On Cm5 Sensor Chipmentioning

confidence: 99%

Generation of a panel of high affinity antibodies and development of a biosensor-based immunoassay for the detection of okadaic acid in shellfish

Berre

Kilcoyne

Kane

2015

Toxicon

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Okadaic acid (OA) and its derivatives, DTX-1 and DTX-2, are marine biotoxins associated with diarrhetic shellfish poisoning. Routine monitoring of these toxins relies on the mouse bioassay. However, due to the technical unreliability and animal usage of this bioassay, there is always a need for convenient and reliable alternative assay methods. A panel of monoclonal antibodies against OA was generated and the most suitable was selected for biosensor-based assay development using surface plasmon resonance. The cross reactivity of the selected antibody with DTX-1 was found to be 73%, confirming the antibody suitability for both OA and DTX detection. The OA and derivative assay was designed as an inhibition assay covering the concentrations 1-75 ng/ml, with a sensitivity of 22.4 ng/ml. The assay was highly reproducible and preliminary validation showed no matrix interference from mussel extracts and good recovery of added standard in mussel extracts, with %CV of <9.3%. This assay could provide a useful and convenient screening tool for OA and its derivatives with a comprehensive extraction protocol for shellfish monitoring programmes.

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Section: Immobilisation Of Oa On Cm5 Sensor Chipmentioning

confidence: 99%

Generation of a panel of high affinity antibodies and development of a biosensor-based immunoassay for the detection of okadaic acid in shellfish

Berre

Kilcoyne

Kane

2015

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“…This system immobilizes progesterone ex-situ by removing the carboxymethyl dextran coated gold sensing surface from the chip cover and dispensing onto it a solution of 3-carboxymethyloxime derivative outside the SPR instrument. It demonstrated a LOD of 3 ng/mL which was later improved to 0.4 ng/mL in cow’s milk [27,28]. This system uses significant dilution to overcome matrix effects [27,28], which can compromise the overall assay sensitivity.…”

Section: Steroidsmentioning

confidence: 99%

Small Molecule Immunosensing Using Surface Plasmon Resonance

Mitchell

2010

Sensors

145

103

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Surface plasmon resonance (SPR) biosensors utilize refractive index changes to sensitively detect mass changes at noble metal sensor surface interfaces. As such, they have been extensively applied to immunoassays of large molecules, where their high mass and use of sandwich immunoassay formats can result in excellent sensitivity. Small molecule immunosensing using SPR is more challenging. It requires antibodies or high-mass or noble metal labels to provide the required signal for ultrasensitive assays. Also, it can suffer from steric hindrance between the small antigen and large antibodies. However, new studies are increasingly meeting these and other challenges to offer highly sensitive small molecule immunosensor technologies through careful consideration of sensor interface design and signal enhancement. This review examines the application of SPR transduction technologies to small molecule immunoassays directed to different classes of small molecule antigens, including the steroid hormones, toxins, drugs and explosives residues. Also considered are the matrix effects resulting from measurement in chemically complex samples, the construction of stable sensor surfaces and the development of multiplexed assays capable of detecting several compounds at once. Assay design approaches are discussed and related to the sensitivities obtained.

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“…With gold nanoparticle enhancement of small molecule SPR immunoassays now well established, it is hoped that it will be successfully applied to an even broader range of compounds. The issue of biofouling in small molecule SPR is also receiving attention but often the techniques used involve total removal of the background matrix by timeconsuming methods such as flow filtering where blockages can occur and LOD are too high (Stevens et al, 2008) or else extensive dilution of samples (Gillis et al, 2002) which worsens the practical LOD. By combining surface and conjugation chemistry with immunology and optics, it is possible to create new sensor interfaces that offer significantly improved detection of small molecules, not only in buffer but also in real biological samples.…”

Section: Context In Wider Plasmonic Biosensor Researchmentioning

confidence: 99%

Surface Plasmon Resonance Biosensors for Highly Sensitive Detection of Small Biomolecules

Mitchell¹,

Wu²

2010

Biosensors

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Development and validation of a biosensor-based immunoassay for progesterone in bovine milk

Cited by 80 publications

References 22 publications

Generation of a panel of high affinity antibodies and development of a biosensor-based immunoassay for the detection of okadaic acid in shellfish

Generation of a panel of high affinity antibodies and development of a biosensor-based immunoassay for the detection of okadaic acid in shellfish

Small Molecule Immunosensing Using Surface Plasmon Resonance

Surface Plasmon Resonance Biosensors for Highly Sensitive Detection of Small Biomolecules

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