Development and Validation of a Gold Nanoparticle Immunochromatographic Assay (ICG) for the Detection of Zearalenone

Abstract

A monoclonal antibody (mAb)-based gold nanoparticle immunochromatographic assay (ICG) for zearalenone detection was developed, optimized, and validated. The detection limits of ICG optimized with appropriate amounts of zearalenone-bovine serum albumin and gold nanoparticle-mAb to zearalenone were 2.5 ng/mL and 30 μg/kg for the standard solution and spike sample, respectively, and a weak cross-reaction for α-zearalenol and β-zearalenol was observed. The assay required only 15 min to obtain results and one step to perform the assay. In validation, the results obtained from spiked corn (10, 20, 30, 50, and 100 μg/kg) and naturally contaminated corn samples by the ICG were in good agreement with those obtained by direct competitive enzyme-linked immunosorbent assay (DC-ELISA) and high-performance liquid chromatography (HPLC). Therefore, the results obtained in this study could be used as basic research for the development of zearalenone-ICG, and the ICG developed could be a useful on-site screening tool for the rapid detection of zearalenone in corn without special instrumentation.

Lateral flow based immunobiosensors for detection of food contaminants

“…In theory, it is possible to add one or more T-line to an existing LFD and to mix the partner labeled antibodies to increase the number of analytes to be determined. Shim et al (Shim, Kim et al 2009) established a system simultaneously to detect OTA, ZEA, OTA and AFB1 in corn by two LFDs. Additionally, multianalytes detection developed by Kolosova et al (Kolosova, De Saeger et al 2007) also confirmed that two analytes can be simultaneously detected by a single LFIA device (Goryacheva, De Saeger et al 2007).…”

Detection of chemical pollutants in water using gold nanoparticles as sensors: a review

“…In any functionalization efforts with DNA and DNA hybridization, charge screening (salt-aging) was necessary to compensate for the electrostatic repulsion between the negatively charged DNA molecules (Hill and Mirkin 2006 ). Immunochromatographic strip assay 0.32 ng/ml, 2.5 ng/ml Bo et al 2007, Liu et al 2008, Shim et al 2009 Table 1 Summary of the detection sensitivity achieved by gold nanoparticle-based sensor platform.…”

“…The color changes associated with NP aggregation, and/or local refractive index change, have been exploited as optical sensing methods for the detection of toxins , Liu et al 2008, Shim et al 2009 ), heavy metals (Darbha et al 2008, Xue et al 2008 ) and other environmental pollutants (Daniel et al 2009, Dasary et al 2009 ) in water, soil and other environmental samples. AuNPs are also being used to enhance the performance of electrochemical sensors due to their catalytic properties (Rassaei et al 2011 ).…”

“…Immunochromatographic strip assays combine chromatography technology with conventional immunoassays, to provide a simple, low-cost and rapid tool for toxins diagnosis, with the aggregation of AuNPs as the colorimetric readout mechanism (Figure 8 ). Recently, many kinds of immunochromatographic assays, using AuNP-antibody conjugates, have been developed for the detection of OTA , Liu et al 2008 ), ZEA (Shim et al 2009 ), and AFB1 (Bo et al 2007 ). The immunochromatographic strip assay has the advantage of an easy operation format, a short assay time and stability over a wide range of conditions, which makes this technique useful for on-site testing by untrained analysts.…”

Biosensors Based on Nanoparticles and Electrochemical Detection

“…In this case, the intensity of the test line is inversely proportional to the amount of analyte present in the sample. 95,96 Due to the diversity and selectivity of antibodies and the advantages of LFIA, this device has been used to determine a huge variety of analytes such as toxins, antibiotics in food, hormones in serum and urine, explosives, etc. [92][93][94][95] Conversely, other molecules can be attached on GNP (nucleic acids, enzymes, aptamers, etc.)…”