Development and validation of a reverse-phase liquid chromatographic method for the assay of lidocaine hydrochloride in alginate-Gantrez® microspheres

Salas, Soumya; Talero, B.; Rabasco, A. M.; González-Rodrı́guez, Marı́a Luisa

doi:10.1016/j.jpba.2008.01.045

Cited by 17 publications

(10 citation statements)

References 20 publications

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“…The introduction of new HPLC methods for routine quality control of pharmaceutical preparations starts with the establishment of the optimal measurement conditions, and provides the maximum relevant information by analyzing the experimental data (García et al, 2003;Pérez-Lozano et al, 2004;Zaxariou and Panderi, 2004;Salas et al, 2008).…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

Protein-loaded PLGA microparticles engineered by flow focusing: Physicochemical characterization and protein detection by reversed-phase HPLC

Holgado

Cózar-Bernal

Salas

et al. 2009

International Journal of Pharmaceutics

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Section: Chromatographic Conditionsmentioning

confidence: 99%

Protein-loaded PLGA microparticles engineered by flow focusing: Physicochemical characterization and protein detection by reversed-phase HPLC

Holgado

Cózar-Bernal

Salas

et al. 2009

International Journal of Pharmaceutics

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“…The chromatographic conditions [19–21] were a column C18 (Waters Spherisorb 5 μ m ODS2; 4.6 × 250 mm Analytical Column, Ireland) and a mobile phase consisting of two solvents: a mixture of acetonitrile : water : acetic acid (A) (75 : 23.7 : 1.3 v/v) and acetonitrile (B). Eluent (70 : 30% A : B) was pumped at 1.000 mL/min.…”

Section: Methodsmentioning

confidence: 99%

Development and Validation of an RP-HPLC Method for CB13 Evaluation in Several PLGA Nanoparticle Systems

Álvarez-Fuentes

Martín-Banderas

Muñoz-Rubio

et al. 2012

The Scientific World Journal

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A simple, fast, and reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for determining of a cannabinoid derivate, which displays potent antihyperalgesic activity, 1-naphthalenyl[4-(pentyloxy)-1-naphthalenyl]methanone (CB13) into PLGA nanoparticles. Separation was achieved in a C18 column using a mobile phase consisting of two solvents: solvent A, consisting of acetonitrile : water : acetic acid (75 : 23.7 : 1.3 v/v), and solvent B, consisting of acetonitrile. An isocratic method (70 : 30 v/v), with a flow rate of 1.000 mL/min, and a diode array detector were used. The developed method was precise, accurate, and linear over the concentration range of analysis with a limit of detection and a limit of quantification of 0.5 and 1.25 μg/mL, respectively. The developed method was applied to the analysis of CB13 in nanoparticles samples obtained by three different procedures (SEV, FF, and NPP) in terms of encapsulation efficiency and drug release. Nanoparticles size and size distribution were also evaluated founding that NPP method presented the most lowest particle sizes with narrow-size distribution (≈320 nm) and slightly negative zeta potential (≈−25 mV) which presumes a suitable procedure for the synthesis of PLGA-CB13 nanoparticles for oral administration.

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“…Various analytical techniques for LD-HCL determination in biological and pharmaceutical samples have been published, including the chromatographic methods,gas chromatography(GC) determination of LD-HCl in whole blood (Keenaghan, 1968), (Edhorn, 1971), (Asada, 1979) (Hattori,Yamamoto, & Yamada, 1991) (Watanabe, Namera, Yashiki, Iwasaki, & Kojima, 1998), from serum (Kruczek, 1981) (Prat , & Bruguerolle, 1986) (Baniceru, Croitoru, & Popescu, 2004), from plsma (Björk , Pettersson, & Osterlöf, 1990), in human plasma and urine (Ohshima& Takayasu, 1999), in human urine (Koster, Hofman, & Jong, 1998), to distinguish the drug from its thermal degradation produts (Stavchansky, Eghbali & Geary, 1987), in pharmaceutical preparations (Tarli P, Benocci & Neri, 1969), and Forced Degradation of Lidocaine HCl by NMR Spectroscopy and GC-FID Methods (Kadioglu, Atila, Serdar, Gultekin, & Alcan, 2013), thin-layer chromatography (TLC)for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation (Dołowy, Kulpińska-Kucia, & Pyka, 2014), high-performance liquid chromatography (HPLC) and TLC (Živanović, Živanov-Stakić, & Radulović, 1998) (Abdelwahab, Nouruddin, Abdelkawy, Emam, 2016), HPLC, in plasma with solid phase extraction and UV detection (Kang, Jun, & McCall, 1999 ), in pharmaceuticals (Smith & Nuessle, 1981) (Waraszkiewicz, Milano, & DiRubio, 1981) (Atay & Öztop, 1997) (Parissi-Poulou & Panderi, 1999) (Gebauer, McClure, & Vlahakis, 2001) (Liawruangrath, Liawruangrath, & Pibool, 2001) (Malenovic, Medenica, Ivanovic, Jancic, & Markovic, 2005) (Zivanovic, Zecevic, Markovic, Petrovic, & Ivanovic, 2005) (Salas, Talero, Rabasco, & González, 2008) (Stojanović, Malenović, Marković, Ivanović, & Medenica, 2010) …”

Section: Introductionmentioning

confidence: 99%

Extraction-Spectrophotometric Determination of Lidocaine Hydrochloride in Pharmaceuticals

Omer¹,

Ali²

2017

IJC

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Simple, selective and highly detectable spectrophotometric method has been developed and validated for the determination of Lidocaine hydrochloride in standard and commercial solutions .The method is based on the formation of a soluble colored Lidocaine hydrochloride-eriochrome black T ion-pair complex at pH 1.80. The colored complex was extracted quantitatively into chloroform and measured at 508 nm. Beer's law was obeyed in the concentration range of 0.10-10 mg L -1 with molar absorptivity of 2.3623 x10 4 L mol -1 cm -1 .The limits of detection and quantification were 0.024 mg L -1 , and 0.100 mg L -1 respectively. Using Job's continuous variations method, the stoichiometry of the ion-pair complex was found to be 1:1. Intra-day accuracy and precision of the method were estimated with a relative error (0.57%), and the relative standard deviation (0.25-1.23). This developed method has been successfully employed to determine concentration of Lidocaine hydrochloride in injection and spray without interference by the common co-formulated substances.The numerical results obtained using both proposed and official methods were in concordance with each other.

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Development and validation of a reverse-phase liquid chromatographic method for the assay of lidocaine hydrochloride in alginate-Gantrez® microspheres

Cited by 17 publications

References 20 publications

Protein-loaded PLGA microparticles engineered by flow focusing: Physicochemical characterization and protein detection by reversed-phase HPLC

Protein-loaded PLGA microparticles engineered by flow focusing: Physicochemical characterization and protein detection by reversed-phase HPLC

Development and Validation of an RP-HPLC Method for CB13 Evaluation in Several PLGA Nanoparticle Systems

Extraction-Spectrophotometric Determination of Lidocaine Hydrochloride in Pharmaceuticals

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