“…Although requiring special reagents and equipment, PCR can detect the presence of specific exogenous genes inserted into the plant genome (including the seeds), and represents the most widely used method to detect GM food and feed (Dinon et al, 2011;Branquinho et al, 2013;Cantelmo et al, 2013). In the present study, we used real time PCR to detect three GM-specific fragments: the promoter region p-35S derived from the cauliflower mosaic virus (Waiblinger et al, 2008;Huber et al, 2013), the terminator region, t-Nos, derived from the nopaline synthase gene of Agrobacterium tumefaciens (Reiting et al, 2007;Huber et al, 2013), and the main cry1A.105 gene from Bt (Dinon et al, 2011). These PCR assays were successfully implemented, in addition to an assay targeting the constitutive hmg gene, which was used as an endogenous control (Corbisier et al, 2010).…”